FORMULATION OF MESENCHYMAL STEM CELL SECRETOME AS ANTIAGING CREAM
DOI:
https://doi.org/10.22159/ijap.2023.v15s1.47506Keywords:
Secretome, Antiaging, Cream, Fibroblast, Fibroblast growth factorAbstract
Objective: Skin aging occurs not only due to age but is influenced by various environmental factors such as lifestyle, pollution, and excessive exposure to UV rays. Secretomes can act as antiaging agents that stimulate collagen biosynthesis naturally in the skin. This study aims to create a cream containing 5% secretome with essential oil, dermatological examination, and Fibroblast Growth Factor (FGF) checking.
Methods: Antiaging efficacy testing involves examination by an expert dermatologist for assessment before and after using the cream. The antiaging impact test criteria include moisture, elasticity, and collagen values. Consequently, the cream shows an antiaging activity.
Results: The antiaging efficacy test revealed increased moisture from 24.375±11.97 to 25.125±7.1; the skin elasticity also increased from 40.375±8.39 to 48.5±9.09 and the collagen value increased from 48.25±13.54 to 56.5±8.63. The result shows that the cream contains a 61.143 pg/ml concentration of FGF using an ELISA kit.
Conclusion: The formula for cream preparations containing 5% secretome with essential oil meets the requirements of cosmetics through several evaluations of the trial. It has an antiaging effect, proven through several tests.
Downloads
References
Vierkotter A, Krutmann J. Environmental influences on skin aging and ethnic-specific manifestations. Dermatoendocrinol. 2012;4(3):227-31. doi: 10.4161/derm.19858, PMID 23467702.
Ramose Silva M, Celem LR, Ramose Silva S, Fucci-da-Costa AP. Anti-aging cosmetics: facts and controversies. Clin Dermatol. 2013;31(6):750-8. doi: 10.1016/j.clindermatol.2013.05.013, PMID 24160281.
Tobin DJ. Introduction to skin aging. J Tissue Viability. 2017 Feb;26(1):37-46. doi: 10.1016/j.jtv.2016.03.002. PMID 27020864.
Yasui H, Sakurai H. Age-dependent generation of reactive oxygen species in the skin of live hairless rats exposed to UVA light. Exp Dermatol. 2003 Oct;12(5):655-61. doi: 10.1034/j.1600-0625.2003.00033.x. PMID 14705807.
Aldag C, Nogueira Teixeira D, Leventhal PS. Skin rejuvenation using cosmetic products containing growth factors, cytokines, and matrikines: a review of the literature. Clin Cosmet Investig Dermatol. 2016 Nov;9:411-9. doi: 10.2147/CCID.S116158. PMID 27877059.
Patel GK, Khan MA, Zubair H, Srivastava SK, Khushman M, Singh S. Comparative analysis of exosome isolation methods using culture supernatant for optimum yield, purity and downstream applications. Sci Rep. 2019;9(1):5335. doi: 10.1038/s41598-019-41800-2, PMID 30926864.
Brandt FS, Cazzaniga A, Hann M. Cosmeceuticals: current trends and market analysis. Semin Cutan Med Surg. 2011 Sep;30(3):141-3. doi: 10.1016/j.sder.2011.05.006. PMID 21925366.
Pawitan JA. Prospect of stem cell conditioned medium in regenerative medicine. BioMed Res Int. 2014;2014:965849. doi: 10.1155/2014/965849, PMID 25530971.
Kim HJ, Jung MS, Hur YK, Jung AH. A study on the clinical effectiveness of cosmetics containing human stem cell conditioned media. Biomed Dermatol. 2020 Dec;4(1):9. doi: 10.1186/s41702-020-0056-9.
Hallstar. Olivem® 1000 the first emulsifying active ingredient. Chicago; 2002.
Fox LT, Gerber M, Du Plessis JD, Hamman JH. Transdermal drug delivery enhancement by compounds of natural origin. Molecules. 2011 Dec;16(12):10507-40. doi: 10.3390/molecules161210507.
Das A, Ahmed AB. Formulation and evaluation of transdermal patch of indomethacin containing patchouli oil as a natural penetration enhancer. Asian J Pharm Clin Res. 2017 Nov;10(11):320. doi: 10.22159/ajpcr.2017.v10i11.20926.
Nadya P, Lucida H, Fitriani L. Optimasi formula krim tabir surya ekstrak buah tomat (Solanum Lycopersicum L.) terpurifikasi menggunakan surfaktan olivem® dan fase minyak VCO. Padang: Universitas Andalas; 2019.
Chen MX, Alexander KS, Baki G. Formulation and evaluation of antibacterial creams and gels containing metal ions for topical application. J Pharm (Cairo). 2016;2016:5754349. doi: 10.1155/2016/5754349. PMID 27885352.
Fatmawati S, Nugrahaeni F, Nursal FK, Fitriana A. Sunscreen factor formulation and test of gel preparations of 70% ethanol extract on arabica coffee leaf (Coffea arabica L.). IOP Conf Ser.: Earth Environ Sci. 2022;1041(1). doi: 10.1088/1755-1315/1041/1/012071.
Mosquera Tayupanta Tdla, Espadero M, Mancheno M, Pena S, Uguna A, Alvarez S. Sensory analysis of cosmetic formulations made with essential oils of Aristeguietia glutinosa (matico) and ocotea quixos (ishpingo). Int J Phytocos Nat Ingred 2018;5(1):5. doi: 10.15171/ijpni.2018.05.
Samanta A, Ojha D, Mukherjee B. Stability analysis of primary emulsion using a new emulsifying agent gum odina. Nat Sci. 2010;2(5):494-505. doi: 10.4236/ns.2010.25062.
Brookfield. Brookfield DIAL VISCOMETER operating instructions (M); 2018.
Matangi PS, Aruna S, Guldan M, Raghavamma S, Rao R. Formulation and evaluation of anti aging poly herbal Cream. Int J Pharm Sci Rev Res. 2014 Jan;24(1):135-6.
Task Force Committee for Evaluation of Anti-Aging Function. Guidelines for the evaluation of anti-wrinkle products (Guidelines for evaluation of cosmetic functions). J Japanesepn Cosmet Sci Soc. 2007;31(4):411-31.
Ermawati D, Chasanah U, Dwi D. Optimization formulation of antioxidant cream vitamin E (a-tocopherol acetate) with virgin coconut oil (VCO); 2017. doi: 10.2991/hsic-17.2017.56.
A Swastika, M Purwanto. Antioxidant activity of cream dosage form of tomato extract (solanum Lycopersicum L.) tradit. Med J. 2015;18(3):132-40.
National Standardization Agency. Sediaan tabir Surya; 1996.
Kale SN, Deore SL. Emulsion microemulsion and nanoemulsion: a review. Syst Rev Pharm. 2016 Nov;8(1):39-47. doi: 10.5530/srp.2017.1.8.
Damayanti RH, Rusdiana T, Wathoni N. Mesenchymal stem cell secretome for dermatology application: a review. Clin Cosmet Investig Dermatol 2021 Oct;14:1401-12. doi: 10.2147/CCID.S331044. PMID 34675575.
Herman A, Herman AP. Essential oils and their constituents as skin penetration enhancer for transdermal drug delivery: a review. J Pharm Pharmacol. 2015;67(4):473-85. doi: 10.1111/jphp.12334, PMID 25557808.
M HJ. Effects on freeze-dried cosmetics made with spicule and stem cell culture media on the skin around eyes of middle-aged women,’ a [Master’s thesis] Konkuk Univ. 2018;54-7, 68-71, 81-4.
De Araujo R, Lobo M, Trindade K, Silva DF, Pereira N. Fibroblast growth factors: A controlling mechanism of skin aging. Skin Pharmacol Physiol. 2019;32(5):275-82. doi: 10.1159/000501145, PMID 31352445.
Published
How to Cite
Issue
Section
Copyright (c) 2023 MARLINA, REYSA PRADIFTA, HENNY LUCIDA, IKHWAN RESMALA SUDJI, HANA NURUL SALSABILA, NUR ELIDA, POPY AYU NAMIRA
This work is licensed under a Creative Commons Attribution 4.0 International License.
