DEVELOPMENT OF GUGGULSTERONE-LOADED PHYTOSOMES: A QUALITY BY DESIGN-BASED CHARACTERIZATION AND OPTIMIZATION STUDIES: DEVELOPMENT OF GUGGULSTERONE-LOADED PHYTOSOMES: A QUALITY BY DESIGN-BASED CHARACTERIZATION AND OPTIMIZATION STUDIES

Damodharan N; Jebastin Koilpillai; Jamal Basha  Dudekula

doi:10.22159/ijap.2024v16i5.51559

Authors

Damodharan N SRM College of Pharmacy
Jebastin Koilpillai Research Scholar, Department of Pharmaceutics, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Chennai 603203
Jamal Basha Dudekula Research Scholar, Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Chennai 603203

DOI:

https://doi.org/10.22159/ijap.2024v16i5.51559

Keywords:

Guggulsterones, gum resin of the Commiphora mukul plant, also known as

Abstract

Objective: The primary objective of this study was to enhance drug delivery efficiency through the design and optimization of guggulsterone-phytosomes, employing a 3-factor 3-level Box-Behnken Design.

Methods: The methodology involved a solvent evaporation technique utilizing guggulsterone and soy lecithin, with a systematic variation and optimization of critical factors such as soy lecithin and guggulsterone concentration, alongside temperature adjustments to refine the phytosome formulations. The characterizations of these formulations were extensive, with a particular emphasis on key quality attributes, notably percentage entrapment efficacy and drug release.

Results: The optimized guggulsterone-phytosomes demonstrated impressive outcomes, showcasing a remarkable entrapment efficiency of 92.64% and a noteworthy drug release rate of 91.69% at 24 hours. These formulations displayed heightened viability in selected cell lines, exhibiting Cellular Toxic Concentration with CTC₅₀ranging from 253.39 to 330.44 µg/ml. Moreover, they exhibited stability under stressed conditions from a physicochemical perspective. The particle size was measured at 137.8 nm, accompanied by a zeta potential of -25.3 mV.

Conclusion: Significantly, the extended drug release from Guggulsterone-phytosomes adhered to first-order kinetics with Fickian diffusion. In summary, this study underscores the efficacy of the Box-Behnken Design in crafting optimized Guggulsterone-phytosomes, showcasing their potential as promising drug delivery carriers. The enhanced drug delivery platform exhibits significant promise in amplifying antihyperlipidemic effects, attributed to the improved performance and stability of these innovative phytosomes

Keywords: Phytosome, Bioavailability, Box–Behnken design, Guggulsterone, Hyperlipidemia, Soy lecithin.

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Author Biographies

Jebastin Koilpillai, Research Scholar, Department of Pharmaceutics, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Chennai 603203

Research Scholar, Department of Pharmaceutics, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Chennai 603203

Jamal Basha Dudekula, Research Scholar, Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Chennai 603203

Research Scholar, Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Chennai 603203

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DEVELOPMENT OF GUGGULSTERONE-LOADED PHYTOSOMES: A QUALITY BY DESIGN-BASED CHARACTERIZATION AND OPTIMIZATION STUDIES