FORMULATION OF SELF-NANO EMULSIFYING SYSTEM (SNES) CONTAINING SNAKEHEAD FISH EXTRACT PROTEIN INCORPORATED INTO CORN OIL BY HYDROPHOBIC ION PAIRING METHOD

GUNAWAN SETIYADI; SUPRAPTO; ENDANG N. WIDIYANINGSIH; AMANDA A. SALSABILA; IZZATUL QUDSIYAH

doi:10.22159/ijap.2024v16s5.52491

Authors

GUNAWAN SETIYADI Pharmaceutics Laboratory, Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia https://orcid.org/0000-0002-6881-687X
SUPRAPTO Pharmaceutics Laboratory, Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia https://orcid.org/0000-0003-4564-1779
ENDANG N. WIDIYANINGSIH Department of Nutrition Science, Faculty of Health Science, Universitas Muhammadiyah, Sukoharjo, Indonesia
AMANDA A. SALSABILA Pharmaceutics Laboratory, Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia
IZZATUL QUDSIYAH Pharmaceutics Laboratory, Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024v16s5.52491

Keywords:

Snakehead fish, Canna striata, Albumin, Proteins, Self-nanoemulsifying system, SNES, Corn oil, Hydrophobic ion pairing

Abstract

Objective: The purpose of this study was to determine whether the protein complexation of snakehead fish extract using the hydrophobic ion pairing method with Sodium Dodecyl Sulfate (SDS) as a complexing ligand could be loaded into the corn oil component of the SNES and to explore the ratio of oil, surfactant, and cosurfactant in the Self-Nanoemulsifying System (SNES) formula that can produce good SNES characteristics.

Methods: Snakehead fish proteins were extracted using pressurized hot water and then complexed with SDS at acidic pH. The SDS-protein complex was loaded into the oil component of the SNES and then combined with the surfactant component (Tween 80) and cosurfactant (propylene glycol) in a ratio of 1:9:1, which, based on the preliminary miscibility screening, has the best miscibility among other screening results.

Results: The results of the study were as follows. The binding efficiency of the SDS-protein-complex was 31.74% and the loading efficiency into SNES was 83.17%. The nanoemulsion formed had visible light transmittance of 100.4%, particle size 72.7 nm, zeta potential-53.03 mV, and emulsification time 71.67 seconds. The nanoemulsion was stable at storage and 100 and 1000 times dilution challenges using aqueous media, Simulated Gastric Fluid (SGF), and Simulated Intestinal Fluid (SIF).

Conclusion: The hydrophobic ion-pair complexation of snakehead fish protein extract with SDS as the complexing ligand was able to load the protein into the oil component of SNES and the ratio of oil, surfactant, and cosurfactant in the SNES formula that produce good SNES characteristics was corn oil, Tween 80, and propylene glycol at a ratio of 1:9:1

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References

Abu Bakar MR, Abdul Kadir A, Abdul Wahab SZ, Abdul Karim AH, Nik Hussain NH, Mohd Noor N. Randomized controlled trial on the effect of channa striatus extract on measurement of the uterus, pulsatility index, resistive index of uterine artery and superficial skin wound artery in post lower segment caesarean section women. Plos One. 2015;10(7):e0133514. doi: 10.1371/journal.pone.0133514, PMID 26222158.

Hanafiah OA, Satria D, Madhy ZA, Wardhani NZ, AAH G, Fajar TH. Effectiveness of cork fish extract gel on fibroblast proliferation in socket wound after tooth extraction. Int J App Pharm. 2024;16(2):34-8. doi: 10.22159/ijap.2024.v16s2.08.

Hirsch KR, Wolfe RR, Ferrando AA. Pre- and post-surgical nutrition for preservation of muscle mass, strength, and functionality following orthopedic surgery. Nutrients. 2021;13(5):1675. doi: 10.3390/nu13051675, PMID 34063333.

Gillis C, Wischmeyer PE. Pre-operative nutrition and the elective surgical patient: why, how and what? Anaesthesia. 2019;74(1)Suppl 1:27-35. doi: 10.1111/anae.14506, PMID 30604414.

Mustafa A, Sujuti H, Permatasari N, Widodo MA. Determination of nutrient contents and amino acid composition of pasuruan channa striata extract. IEESE Int J Sci Technol. 2013;2(4):1-11.

Cialdai F, Risaliti C, Monici M. Role of fibroblasts in wound healing and tissue remodeling on earth and in space. Front Bioeng Biotechnol. 2022;10:958381. doi: 10.3389/fbioe.2022.958381, PMID 36267456.

Winarti L. Formulation of self-nanoemulsifying drug delivery system of bovine serum albumin using HLB (Hydrophilic-Lypophilic Balance) approach. Indonesian J Pharm. 2016;27(3):117. doi: 10.14499/indonesianjpharm27iss3pp117.

Sakloetsakun D, Dünnhaupt S, Barthelmes J, Perera G, Bernkop Schnürch A. Combining two technologies: multifunctional polymers and self-nanoemulsifying drug delivery system (SNEDDS) for oral insulin administration. Int J Biol Macromol. 2013;61:363-72. doi: 10.1016/j.ijbiomac.2013.08.002, PMID 23933302.

Lal B, Gadewar M, Garg A. Development and evaluation of self-emulsified drug delivery system of embelin for the treatment of amoebiasis. Int J App Pharm. 2022;14(3):141-52. doi: 10.22159/ijap.2022v14i3.44222.

Lestari R.G. and Sukmawati A., 2024, Study of Liquid Self-nanoemulsifying Drug Delivery System ( L- SNEDDS ) that Use Oleic Acid as the Oil Phase : Literature Review, Pharmacon: Jurnal Farmasi Indonesia, 21 (1), 124–134.

Gottemukkula LD, Sampathi S. SNEDDS as lipid-based nanocarrier systems: concepts and formulation insights. Int J App Pharm. 2022;14(2):1-9. doi: 10.22159/ijap.2022v14i2.42930.

Ristroph KD, Prud’homme RK. Hydrophobic ion pairing: encapsulating small molecules, peptides, and proteins into nanocarriers. Nanoscale Adv. 2019;1(11):4207-37. doi: 10.1039/c9na00308h, PMID 33442667.

Devrim B, Bozkır A. Design and evaluation of hydrophobic ion-pairing complexation of lysozyme with sodium dodecyl sulfate for improved encapsulation of hydrophilic peptides/proteins by lipid-polymer hybrid nanoparticles. J Nanomed Nanotechnol. 2015;6(1):259.

Suhendi A, Rohman A, Cahyaningrum S. Validation of method analysis on determination protein level snakehead fish extract by lowry and bromocresol green method. J Kefarmasian Indones. 2023;13(1):50-8.

Griesser J, Hetényi G, Moser M, Demarne F, Jannin V, Bernkop Schnurch A. Hydrophobic ion pairing: key to highly Payloaded self-emulsifying peptide drug delivery systems. Int J Pharm. 2017;520(1-2):267-74. doi: 10.1016/j.ijpharm.2017.02.019, PMID 28188875.

Winarti L, Martien R, Suwaldi HL. An experimental design of SNEDDS template loaded with bovine serum albumin and optimization using D-optimal. Int J Pharm Clin Res. 2016;8(5):425-32.

Wikantyasning ER, Setiyadi G, Pramuningtyas R, Kurniawati MD, Ho CY. Formulation of nanoemulgel containing extract of impatients balsamina l. and its antibacterial activity. Int J Appl Pharm. 2023;15(3):67-70.

Hamrah KT, Al-Shaibani AJ, Abdulrazzaq FI. Preparation and evaluation of lafutidine nanoemulsion as oral delivery system. Int J Appl Pharm. 2024;16(3):342-8.

Dwijayanti DR, Djati MS, Rifa’I M. The role of VipAlbumin(®) as an immunostimulatory agent for controlling homeostasis and proliferation of lymphoid cells. Cent Eur J Immunol. 2016;41(1):31-8. doi: 10.5114/ceji.2016.58814, PMID 27095920.

Suryanti S, Marseno DW, Indrati R, Irianto HE. Effect of Acid Type in Isolation of Gelatin from Nila Fish (Oreochromis niloticus) Skin on Characteristics of Emulsion. Agritech. 2018;37(4):410. doi: 10.22146/agritech.13025.