EFFICACY OF AQUEOUS AND METHANOLIC EXTRACTS OF PLANT BRASSICA RAPA LEAVES POTENTIAL ANTI-PSEUDOMONAL ACTIVITY
DOI:
https://doi.org/10.22159/ijcpr.2022v14i1.44115Keywords:
Brassica rapa, Anti-pseudomanal activities, Agar well diffusion method, Gentamycin, Phytochemical analysis, Turnip greenAbstract
Objective: Efficacy of Aqueous and Methanolic Extracts of Plant Brassica Rapa Leaves Potential Anti-Pseudomanal Activity
Methods: Phytochemical analysis of Brassica rapa belonging to the family was examined using Aqueous and Methanolic extracts. Methanolic and aqueous extracts of leaves part of the plant were investigated individually for Anti-pseudomanal activity by agar well diffusion method. The susceptibility of three gram-negative bacteria to the aqueous and Methanolic extracts of this plant was measured and the results compared with standard antibiotic Gentamycin. Both the extracts were tested against selected species of Pseudomonas aeruginosa MTCC2295 (G-ve), Pseudomonas fluorescens MTCC2421 (G-ve), and Pseudomonas alcaligenes MTCC7095 (G-ve), to find the inhibitory activities of microbes at the dose level of 50 and 100 µg/ml.
Results: The phytochemical analysis of Aqueous and Methanolic extracts confirmed the presence of Carbohydrates, Fat, proteins, Vitamins, Minerals, Electrolytes, sugar, soluble and insoluble fiber and amino acids. Methanolic extract of Brassica rapa showed considerably high Anti-pseudomonal activities against all bacteria than Aqueous extract.
Conclusion: The leaves of Brassica Rapa were found to be rich in sulphur and nitrogen-containing compounds. Brassicarapamethanolic extract has anti-pseudomonal effects against bacteria.
The current study's range of activity can provide a new plant source for safe anti-pseudomanal drugs.
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