CHARACTERIZATION AND ANTIMICROBIAL SPECTRUM OF A POTENT STREPTOMYCES SP. GOS2 ISOLATED FROM WESTERN GHATS OF KARNATAKA, INDIA
DOI:
https://doi.org/10.22159/ijpps.2016.v8i9.12547Keywords:
Streptomyces, Western Ghats, Antibiotics, Macrolides, UV spectroscopyAbstract
Objective: Western Ghats of Karnataka, India-a biodiversity hotspot is a rich source of microorganisms with undiscovered metabolic capabilities. The upsurge of antibiotic-resistant pathogens has created a greater demand for novel antibiotics. In context to the above a potent soil Streptomyces sp. GOS2 isolated from Agumbe regions of Western Ghats, and its metabolite was assessed and characterized for its antimicrobial spectrum.
Methods: The isolation was carried out by soil serial dilution plating on Starch Casein Nitrate agar media (SCN). The obtained isolate was characterized by morphological and biochemical tests. The antimicrobial activity was assessed by well in agar methods against 28 test organisms. The partial characterization of the bioactive metabolite was carried out by thin layer chromatography (TLC) and UV–Visible spectroscopy studies.
Results: The isolated GOS2 was observed as a raised powdery colony with grey colored aerial mycelium and media impregnated substrate mycelium. The spore chain was rectus with smooth spore surface. The isolate was gram positive, non-acid fast, positive for catalase, hydrogen sulphide production and starch hydrolysis, negative for casein and gelatin hydrolysis. The carbohydrate fermentation studies showed acid production in dextrose and alkali production in sucrose, lactose, maltose and starch. A prominent antibacterial activity was observed with a zone of inhibition measuring 21-27 mm. The TLC showed a purple spot and UV spectroscopy revealed λ max at 233.2 and 235 nm indicating macrolide group of antibiotics.
Conclusion: Western Ghats actinomycetes are a potent source of novel antibiotic molecules.
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