OPTIMIZATION OF MEDIUM COMPONENTS FOR ANTIBACTERIAL METABOLITE PRODUCTION FROM MARINE STREPTOMYCES SP. PUA2 USING RESPONSE SURFACE METHODOLOGY
Keywords:
Antibacterial activity, Marine actinobacteria, Central Composite Design, Plackett Burmann design, Response surface methodologyAbstract
Objective: The present study is an attempt to optimize the fermentation conditions for the antibacterial compound production from a newly isolated marine Streptomyces strain PUA2 by adopting response surface methodology as the statistical tool.
Methods: Prior to using the Response Surface Methodology, Plackett Burmann (PB) design was used to explore the effect of variables on the antibacterial compound production. In PB method, high and low values were assigned for the eight variables viz., glucose, glycerol, soybean meal, manganese chloride, calcium carbonate, peptone and pH. Calcium carbonate and peptone were used as dummy variables. Based on the results of combined effects glycerol, soybean meal, manganese chloride and pH were investigated by 24 full-factorial central composite design.
Results: The results of PB method showed the significant effect of glycerol, soybean meal, manganese chloride and pH on the antibacterial compound production. The results of ANOVA and regression of second order model showed that the linear effects of glycerol and manganese chloride and cross products effects of manganese chloride and pH were more significant. All the critical variables having greatest effect on the production of antibacterial compound from marine Streptomyces species PUA2. Optimization of process parameters resulted in increase in antibacterial activity from 7 mm to 14 mm.
Conclusion: The factors optimized in the present study were useful for the increased production of antibacterial metabolite from Streptomyces sp PUA2. The result confirms the feasibility of medium optimization to improve antibiotic production.
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