WOUND HEALING POTENTIAL OF ACACIA CATECHU IN EXCISION WOUND MODEL USING IN VITRO AND IN VIVO APPROACH

ARCHANA TIWARI; AVINASH TIWARI; SANGEETA SHARMA

doi:10.22159/ijpps.2023v15i12.49539

Authors

ARCHANA TIWARI Department of Botany, Government P. G. College Guna-473001, M. P., India https://orcid.org/0000-0001-5713-995X
AVINASH TIWARI School of Studies in Botany, Jiwaji University, Gwalior-474011, M. P., India
SANGEETA SHARMA School of Studies in Botany, Jiwaji University, Gwalior-474011, M. P., India

DOI:

https://doi.org/10.22159/ijpps.2023v15i12.49539

Keywords:

Acacia catechu, Wound healing, Bark extract, Acute dermal toxicity, Bioactive compounds, Catechin

Abstract

Objective: The objective of this work is to elucidate the wound healing capabilities of various extracts derived from the bark of Acacia catechu, and to explore their potential therapeutic effects. Furthermore, the examination of several seasons has been conducted to assess their influence on the examined parameters.

Methods: In this study, albino mice were used to assess acute dermal toxicity, excision wound healing, and histological changes. The wounds were monitored, and the area of the wound was measured at the 0^th, 4^th, 8^th, 12^th, 16^th, and 21^st days as compared to the control animals.

Results: The test extracts showed considerable protection and wound healing capabilities in acute dermal toxicity, excision wound, and histopathological studies. Among the tested extracts, the ethanolic extract showed the highest wound healing (46.68%, p<0.001), followed by the methanolic (38.50%, p<0.001), acetone (33.87%, p<0.05), aqueous (32.04%, p<0.001), chloroform (29.83%, p<0.05), and benzene extracts (28.60%, p<0.05) at the 21^st d of wound healing. However, the samples gathered throughout the winter, summer, and rainy seasons did not show a statistically significant difference (p>0.05) in the present research work.

Conclusion: This study helps to provide preliminary data on the concentration range of different extracts collected in different seasons. It is anticipated that this evaluation represents significant protective potential of indigenous flora for medicinal applications.

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