AN ASSESSMENT OF THE RELATIVE ANTIOXIDANT ACTIVITY OF BARK EXTRACTS OF ACACIA CATECHU BY IN VITRO FREE RADICAL SCAVENGING ANALYSIS

ARCHANA TIWARI; AVINASH TIWARI

doi:10.22159/ijpps.2024v16i1.49763

Authors

ARCHANA TIWARI Department of Botany, Government P. G. College Guna-473001, (M. P.), India
AVINASH TIWARI Vice Chancellor, Jiwaji University, Gwalior-474011, (M. P.), India

DOI:

https://doi.org/10.22159/ijpps.2024v16i1.49763

Keywords:

Nitric oxide, Catechin, Acacia catechu bark extract, Antioxidant, Free radical scavenging, Season-dependent

Abstract

Objective: The objective of this research was to examine and compare the capacity of several bark extracts of Acacia catechu to scavenge nitric oxide (NO) free radicals. The study also examined the evaluation of variations in concentration that are reliant on both concentration levels and seasonal changes, using samples obtained throughout various seasons over a span of two consecutive years.

Methods: In this study, six extracts were made utilizing solvents, including ethanol, methanol, aqueous solution, acetone, chloroform, and benzene. In the in vitro investigation, a nitric oxide (NO) assay was conducted to evaluate the free radical scavenging efficacy of the test samples.

Results: Out of seven tested sample concentrations, 15.25 µg/ml was reported to be ineffective; higher than 500 µg/ml concentrations (i.e., 705 and 1000) were observed to be less effective than their lower concentrations, while 31.5–500 µg/ml drug concentrations were observed to be protective. Among these three, 125 µg/ml concentrations were found to be most effective (p<0.01 or more). In solvent-based results, methanolic, ethanolic, aqueous, and acetone extracts exhibited at least p<0.01 significant effective NO scavenging, but acetone extract was seen to have comparatively less protection (p<0.05) than the other three extracts. Chloroform and benzene extracts, respectively, showed less protection. Samples collected in the summer season showed greater protection than winter and Manson.

Conclusion: This study provided a clear observation of the impact of extraction solvent, concentration of drug, and season of sample collection on in vitro free radical scavenging potential. These data could help provide possible applications for regional plants for medicinal purposes.

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References

Ingle KP, Deshmukh AG, Padole DA, Dudhare MS, Moharil MP, Khelurkar VC. Phytochemicals: extraction methods, identification, and detection of bioactive compounds from plant extracts. J Pharmacogn Phytochem. 2017;6(1):32-6.

Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength, and limitation. Med Aromat Plants. 2015;4(3):1000196.

Sofowora A. The present status of knowledge of the plants used in traditional medicine in western Africa: a medical approach and a chemical evaluation. J Ethnopharmacol. 1980;2(2):109-18. doi: 10.1016/0378-8741(80)90004-5, PMID 7453197.

Pandey A, Tripathi S. Concept of standardization, extraction, and pre-phytochemical screening strategies for herbal drug. J Pharmacogn Phytochem. 2014;2(5):115-9.

Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga Latha L. Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr J Tradit Complement Altern Med. 2011;8(1):1-10. doi: 10.4314/ajtcam.v8i1.60483, PMID 22238476.

Das K, Tiwari RK, Shrivastava DK. Techniques for evaluation of medicinal plant products as antimicrobial agents: current methods and future trends. J Med Plants Res. 2010;4(2):104-11.

Reddy MBK, Gowda KPS, Arora AK. Study of wound healing activity of aqueous and alcoholic bark extracts of Acacia catechu on rats. J Pharm Sci. 2011;1(3):220-5.

Tiwari A, Tiwari A. Amazing antimicrobial and wound healing potential of acacia catechu bark extracts-a review. Asian J Pharm Clin Res. 2021;4(10):1-7. doi: 10.22159/ajpcr.2021.v14i10.43009.

Jatav BK, Sharma TK, Dassani S. Succession of microfungi on leaf litter of Acacia catechu in Datia, Madhya Pradesh, India. J Pure Appl Microbiol. 2020;14(1):581-90. doi: 10.22207/JPAM.14.1.60.

Gajera HP, Patel SV, Golakiya BA. Antioxidant properties of some therapeutically active medicinal plants-an overview. J Med Aromat Plant Sci. 2005;27:91-100.

Rungsung W, Ratha KK, Dutta S, Dixit AK, Hazra J. Secondary metabolites of plants in drugs discovery. World J Pharm Res. 2015;4(7):604-13.

Rimando AM, Olofsdotter M, Dayan FE, Duke SO. Searching for rice allelochemicals: an example of bioassay-guided isolation. Agron J. 2001;93(1):16-20. doi: 10.2134/agronj2001.93116x.

Pal SK, Shukla Y. Herbal medicine: current status and the future. Asian Pac J Cancer Prev. 2003;4(4):281-8. PMID 14728584.

Sahu TR. An ethnobotanical study of Madhya Pradesh 1: plants used against various disorders among tribal women. Anc Sci Life. 1982;1(3):178-81. PMID 22556487.

Tiwari DK, Yadav A. Ethnobotanical investigation of some medicinal plants availed by Gond tribe of Naoradehi Wild Life Sanctuary, Madhya Pradesh. Anthropologist. 2003;5(3):201-2. doi: 10.1080/09720073.2003.11890805.

Agrawal J, Kar A. Chronic treatment of Diabecon in the regulation of alloxan-induced hyperglycemia and oxidative stress in different tissues of adolescent diabetic rats. Int J Pharm Pharm Sci. 2014;6(4):83-7.

Agrawal J, Kar A, Panda S. Synergistic action of phytochemicals augments their antioxidative efficacy: an in vitro comparative study. Asian J Pharm Clin Res. 2013;6(8):121-6.

Chaudhari SK, Shalini T, Singh DP, Verma NK, Chandra VA. An overview on Acacia catechu. Int J Res Rev Pharm Appl Sci. 2012;2(2):342-6.

Bimla M, Chander J, Kalidhar SB. A review on the chemistry and bioactivity of Acacia spp. J Med Aromatic Plants Sci. 2005;27(1):51-90.

Rai R. Promising medicinal plants their parts and formulations prevalent in folk medicines among ethnic communities in Madhya Pradesh, India. PPIJ. 2017;5(3):99-106. doi: 10.15406/ppij.2017.05.00122.

Jain AK, Vairale MG, Singh R. Folklore claims on some medicinal plants used by Bheel tribe of Guna district Madhya Pradesh. Indian J Trad Knowl. 2010;9(1):105-7.

Shah J, Patel S, Bhairy S, Hirlekar R. Formulation optimization, characterization and in vitro anti-cancer activity of curcumin loaded nanostructured lipid carriers. Int J Curr Pharm Sci. 2022;14(1):31-43. doi: 10.22159/ijcpr.2022v14i1.44110.

Avalappa H, Padiyappa SD, Pathappa N, Bettadatunga PT, Pramod SN. Legume Indian Bean (Phaseolus vulgaris) partially purified anti-nutritional protein factor exhibit anti-oxidant, immunomodulatory and anti-cancer properties. Int J Pharm Pharm Sci. 2023;15(2):8-17. doi: 10.22159/ijpps.2023v15i2.46831.

Sasikala P, Pugazhendy K. Antioxidant and cytotoxic agent from Acacia catechu stem bark against Mcf-7 cell line. AJBLS. 2022;11(1):77-81. doi: 10.5530/ajbls.2022.11.11.

Baluja S, Chanda S, Solanki A, Kachhadia N. Phytochemical studies of Acacia catechu. Indonesian J Pharm. 2012;23(4):238-47.

Negi BS, Dave BP. In vitro antimicrobial activity of Acacia catechu and its phytochemical analysis. Indian J Microbiol. 2010;50(4):369-74. doi: 10.1007/s12088-011-0061-1, PMID 22282602.

Kumar U, Mishra M, Prakash V. Assessment of antioxidant enzymes and free radical scavenging activity of selected medicinal plants. Free Radic Antioxid. 2012;2(3):58-63. doi: 10.5530/ax.2012.3.8.

Seal T, Pillai B, Chaudhuri K. Effect of cooking methods on total phenolics and antioxidant activity of selected wild edible plants. Int J Pharm Pharm Sci. 2023;15(7):20-6. doi: 10.22159/ijpps.2023v15i7.48179.