THE MEASUREMENT OF CEFOTAXIME SODIUM IN RAT PLASMA AFTER ORAL ADMINISTRATION: A SENSITIVE HPLC-UV METHOD
Keywords:
Cefotaxime sodium, HPLC-UV assay, Validation, Stability, Plasma concentrationAbstract
Objective: To develop and validate a high-performance liquid chromatographic method for the determination of cefotaxime sodium (NaCTX) concentration in rat plasma.
Methods: The method used direct injection of the plasma supernatant after deproteinization with perchloric acid. Degradation of NaCTX in acidic medium during sample treatment was retarded by reducing the strength of perchloric acid to 30%, followed by adding 0.55M of aqueous disodium hydrogen orthophosphate buffer before centrifuging the sample. The mobile phase used was consisted of 0.04M aqueous ammonium acetate: acetonitrile: tetrahydrofurane (90:7:3, v/v) with pH adjusted to 5.7 using glacial acetic acid. The flow rate was 0.9 ml/min, UV detector set at 254 nm and samples were quantified using peak area
Results: A well-resolved NaCTX peak and free of interference from endogenous compounds in rat plasma were achieved. Recovery of NaCTX was satisfactory over the concentration range tested 0.125-10 µg/ml. limit of quantification (LOQ) of this assay was 0.125 µg/ml and, at this concentration, intra-and inter-day CV were 5.33 and 6.13 %, respectively. NaCTX was found to be stable in rat plasma after storage at-80 °C, over 90 days. The plasma concentration-time profile in rats for NaCTX after oral administration of NaCTX solution was achieved using the present method.
Conclusion: The stability, sensitivity, specificity and reproducibility of this method make it suitable for the determination of NaCTX plasma concentration in pharmacokinetics and bioavailability studies.
Â
ÂDownloads
References
Rosseel MT, Vandewoude KH. Liquid chromatographic determination of the plasma concentrations of cefotaxime anddesacetylcefotaxime in plasma of critically ill patients. Chromatogr B: Anal Technol Biomed Life Sci 2004;811:159-63.
Jehl F, Birckel P, Monteil H. Hospital routine analysis of penicillins, third-generation cephalosporins and aztreonam byconventional and high-speed high-performance liquid chromatography. J Chromatogr 1987;413:109–19.
Sharma P, Chawla HP, Panchagnula R. LC determination of cephalosporins in vitro rat intestinal sac absorption model. J Pharm Biomed Anal 2002;27:39–50.
Rosseel MT, Vandewoude KH. Liquid chromatographic determination of the plasma concentrations of cefotaxime and desacetylcefotaxime in plasma of critically ill patients. J Chromatogr B: Anal Technol Biomed Life Sci 2004;81:159–63.
Samanidou VF, Ioannou AS, Papadoyannis IN. The use of a monolithic column to improve the simultaneous determination of four cephalosporin antibiotics in pharmaceuticals and body fluids by HPLC after solid phase extraction-a comparison with a conventional reversed-phase silica-based column. J Chromatogr B: Anal Technol Biomed Life Sci 2004;809:175–82.
Scanes T, Hundt AF, Swart KJ, Hundt HK. Simultaneous determination of cefotaxime and desacetylcefotaxime in human plasma and cerebrospinal fluid by high-performance liquid chromatography. J Chromatogr B: Biomed Sci Appl 2004;750:171–6.
Dell D, Chamberlain J, Coppin F. Determination of cefotaxime and desacetylcefotaxime in plasma and urine by high performance liquid chromatography. J Chromatogr 1981;226:431–40.
Lecaillon JB, Rouan MC, Souppart C, Febvre N, Juge F. Determination of cefsulodin, cefotiam, cephalexin, cefotaxime, desacetyl-cefotaxime, cefuroxime and cefroxadin in plasma and urine by high-performance liquid chromatography. J Chromatogr 1982;228:257–67.
Bafeltowska J, Buszman E, Mandat K, Hawranek J. Determination of cefotaxime and desacetylcefotaxime in cerebrospinal fluid by solidphase extraction and high-performance liquid chromatography. J Chromatogr A 2002;976:249–54.
Bergan T, Solberg R. Assay of cefotaxime by high-pressure-liquid chromatography. Chemother 1981;27:155–65.
Patel KB, Nicolau DP, Nightingale CH, Quintiliani R. Comparative serum bactericidal activities of ceftizoxime and cefotaxime against intermediately penicillin-resistant Streptococcus pneumoniae. Antimicrob Agents Chemother 1996;40:2805–8.
Signs SA, File TM, Tan JS. High-pressure liquid chromatographic method for analysis of cephalosporins. Antimicrob Agents Chemother 1984;26:652–5.
Richard LY, Hartmut D. Rapid chromatographic determination of cefotaxime and its metabolite in biological fluids. J Chromatogr 1985;341:131–8.
Golocorbin-Kon S, Mikov M, Arafat M, Lepojevic Z, Mikov I, Sahman-Zaimovic M. Cefotaxime pharmacokinetics after oral application in the form of 3alpha,7alphadihydroxy-12-keto-5beta-cholanate microvesicles in rat. Eur J Drug Metab Pharmacokinet 2009;34:31–6.
Yang L, Zhang H, Mikov M, Tucker IG. Physicochemical and biological characterization of monoketocholic acid, a novel permeability enhancer. Mol Pharm 2009;6:448–56.
Ling SS, Yuen KH, Barker SA. Simple liquid chromatographic method for the determination of cefotaxime in human and rat plasma. J Chromatogr B: Anal Technol Biomed Life Sci 2003;783:297–301.
Fabre H, Eddine NH, Berge G. Degradation kinetics in aqueous solution of cefotaxime sodium, a third-generation cephalosporin. J Pharm Sci 1984;73:611–8.
Berge SM, Henderson NL, Frank MJ. Kinetics and mechanism of degradation of cefotaxime sodium in aqueous solution. J Pharm Sci 1983;72:59–63.
Das GV. Stability of cefotaxime sodium as determined by high-performance liquid chromatography. J Pharm Sci 1984;73:565–7.