CELL CYCLE ARREST ACTIVITY OF LITSEA CUBEBA LOUR: HEARTWOOD AND FRUIT EXTRACTS AGAINST T47D BREAST CANCER CELLS

Authors

  • Aminah Dalimunthe Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.
  • Poppy Anjelisa Z Hasibuan Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.
  • Denny Satria Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i11.20204

Keywords:

Cell cycle arrest, Litsea cubeba, Heartwood, Fruit, Extract

Abstract

 

 Objective: This study was carried out to investigate cell cycle arrest activity toward T47D cells of Litsea cubeba heartwoods and fruits extract.

Methods: Dry extracts were prepared from dry-grounded heartwoods and fruits by cold maceration using n-hexane, ethylacetate, and ethanol solvent. Cytotoxic activity were measured by [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide] methods and interpreted with inhibitory concentration 50% (IC50) value. Cell cycle arrest was investigated by flowcytometry method.

Results: IC50 values for each n-hexane, ethylacetate, and ethanol of L. cubeba heartwoods and fruits were 76.34±2.61; 67.52±2.45; 71.23±2.37; 75.59±3.24; 162.58±15.08; 63.70±2.67 μg/mL, respectively. Cell cycle arrest for ethylacetate extract of heartwoods and fruits were accumulated in G0/G1 phase (56.70% and 65.56%).

Conclusion: These results suggest that L. cubeba heartwoods and fruits has cell cycle arrest activity.

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Author Biography

Aminah Dalimunthe, Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.

Department of Pharmaceutical Biology

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Published

01-11-2017

How to Cite

Dalimunthe, A., P. A. Z. Hasibuan, and D. Satria. “CELL CYCLE ARREST ACTIVITY OF LITSEA CUBEBA LOUR: HEARTWOOD AND FRUIT EXTRACTS AGAINST T47D BREAST CANCER CELLS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 11, Nov. 2017, pp. 404-6, doi:10.22159/ajpcr.2017.v10i11.20204.

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