NEPHROPROTECTIVE ACTIVITY OF ETHANOL EXTRACT ROOT OF COGON GRASS (IMPERATA CYLINDRICA L. (BEAUV.)) ON CREATININE, UREA LEVELS, AND HEMATOLOGY PROFILE AGAINST GENTAMYCIN-INDUCED RENAL TOXICITY IN RATS

Authors

  • Aninditha Rachmah Ramadhiani Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Urip Harahap Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Aminah Dalmunthe Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26578

Keywords:

Gentamicin, Cogon grass, Imperata cylindrica L (Beauv), Creatinine, Urea, Profile of hematology

Abstract

 Objective: This study aimed to evaluate the effect of ethanol extract root of cogon grass on parameter creatinine, urea levels, and hematology profile in rats induced by gentamicin.

Methods: Thirty rats were divided into 6 groups of normal group (without treatment), groups of treatment only fed and watered, given carboxymethylcellulose (CMC) 0.5%, ethanol extract of the roots of cogon grass with each dose of 100, 200, and 400 mg/kg BW. Each group was given the test preparation orally for 8 days. After 1 h, 5 groups were induced by gentamicin at a dose 100 mg/kg bw intraperitoneally during 8 days. On the 9th day, blood samples were collected for serum creatinine, urea levels, and hematological parameters; hemoglobin (Hb), red blood cell, and packed cell volume.

Results: Ethanol extract root of cogon grass doses of 100 mg/kg bw, 200 mg/kg bw, and 400 mg/kg BW significantly decreased serum creatinine and urea levels, significantly increased hematological parameters as compared to negative control group of CMC 0.5% and negative control group not given CMC 0.5% (p<0.05). Ethanol extract root of cogon grass at a dose of 400 mg/kg bw showed there were not differences of creatinine, urea levels, and profile of hematology compared with normal group (p>0.05).

Conclusion: The ethanol extract root of cogon grass at a dose of 400 mg/kg bw was effective to ameliorate creatinine, urea levels, and hematological parameters.

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References

Gelaw BK, Tegegne GT, Defersha AD, Legese A. Retrospective drug use evaluation of gentamycin use in ambo hospital, oromia region state,West Showa, Ethiopia. Indian J Pharm Pract 2014;7:20-1.

Chatterjee P, Aniruddha M, Subhangkar N. Protective effects of the aqueous leaf extract of Aloe barbadensis on gentamicin and cisplatin-induced nephrotoxic rats. Elsevier. Asian Pac J Trop Biomed 2012;5:1754-63.

Novoa JM, Quiros Y, Vicente L, Morales AI, Hernandez FJ. New insights into the mechanism of aminoglycoside nephrotoxicity: An intergrative point of view. Int Soc Nephrol 2010; ???:1-4.

Naeshiro I, Ishimura Y, Chatani F, Sato S. Possible mechanism for the anaemia induced by gentamicin in rats. Original Article. Comp Haematol Int 1997;7:220-5.

Parvathy NG, Padma R, Renjith V, Rahate KP, dan Saranya TS. Phytochemical screening and anthelmintic activity of methanolic extract of Imperata cylindrica. Int J Pharm Pharm Sci 2011;4:233-4.

Poblete J. Nephroprotective Effects of Cogon (Imperata cylindrica) Root Aqueous Extract on Sprague-Dawley Rats with Gentamicin- Induced Acute Kidney Injury. ???: Research and Innovation In Health For Disaster and Emergency Management 2014. p. 110-5.

Dahal A, Sirisha M. Flavonoids in kidney protection. review article. World J Pharm Pharm Sci 2015;4:362-5.

Gilbert DN, Wood CA, Kohlhepp SJ, Kohnen PW, Houghton DC, Finkbeiner HC, et al. Polyaspartic acid prevents experimental aminoglycoside nephrotoxicity. J Infect Dis 1989;159:945-53.

Geetha K, Ramarao N, Sindhu B, Rao VU. Nephroprotective, nephrocurative activity of Mimosa pudica root against gentamicin induced nephrotoxicity. Int J Pharm Pharm Sci 2015;7:173-7.

Howard TE. Clinical Chemistry. New York: John Wiley and Sons; 1989. p. 4, 58.

Alfonso AA, Mongan AE, Memah MF. Serum creatinin level description on 5th stage non dialysis Kidney Disease’s patients. J e-Biomedik 2016;4:178-83.

Gowda S, Desai PB, Kulkarni SS, Hull VV, Math AA, Vernekar SN, et al. Markers of renal function tests. N Am J Med Sci 2010;2:170-3.

Badwi SM, Bakhiet AO, Gadir EH. Haemato-biochemical effect of aqueous extract of Kanaya senegalensis stem bark on gentamicin-induced nephrotoxicity in wistar rats. J Biol Sci 2012;12:1-4.

Tangkiatkumjai M. existing evidence for safe use of herbal medicines in chronic kiney disease. review article. Int J Pharm Pharm Sci 2015;7:30- 4.

Published

26-04-2018

How to Cite

Ramadhiani, A. R., U. Harahap, and A. Dalmunthe. “NEPHROPROTECTIVE ACTIVITY OF ETHANOL EXTRACT ROOT OF COGON GRASS (IMPERATA CYLINDRICA L. (BEAUV.)) ON CREATININE, UREA LEVELS, AND HEMATOLOGY PROFILE AGAINST GENTAMYCIN-INDUCED RENAL TOXICITY IN RATS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 13, Apr. 2018, pp. 97-99, doi:10.22159/ajpcr.2018.v11s1.26578.

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