IN VITRO ANTIBACTERIAL ACTIVITY OF THE ETHANOLIC EXTRACT OF JALOH (SALIX TETRASPERMA ROXB.) LEAVES AGAINST STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA

Fitrah Wahyuni; Urip Harahap; Masfria Masfria

doi:10.22159/ajpcr.2018.v11s1.26580

Authors

Fitrah Wahyuni Master Programme in Pharmacy, Faculty of Pharmacy, University of Sumatera Utara, Jalan Tri Dharma No. 5, Padang Bulan, Medan, Indonesia.
Urip Harahap Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Jalan Tri Dharma No. 5, Padang Bulan, Medan, Indonesia.
Masfria Masfria Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sumatera Utara, Jalan Tri Dharma No. 5, Padang Bulan, Medan, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26580

Keywords:

Antibacterial, Salix tetrasperma Roxb, Jaloh, Staphylococcus aureus, Pseudomonas aeruginosa

Abstract

Objective: This study aims to determine antibacterial activity of ethanolic extract of jaloh (Salix tetrasperma Roxb.) leaves against Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA).

Methods: Extract was obtained by maceration method of jaloh (S. tetrasperma Roxb.) leaves dried powder with 96% ethanol as solvent. The antibacterial activities of extract were tested by Kirbyâ€“Bauer method against SA and PA. Data were analyzed statistically using Kruskalâ€“Wallis test for significant difference level p<0.05.

Results: Based on the regression test, the equation of regression curve of extract antibacterial activity on SA and PA, respectively, was y=350.456x-229.579 and y=331.866x-272.069. The minimum inhibitory concentrations (MICs) of SA and PA from the equation of regression curve, respectively, were 4.5193 and 6.6039 mg/mL.

Conclusion: Based on the MIC value, ethanolic jaloh leaves extract had a weak antibacterial activity against SA and PA.

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