A NOVEL OF BEZAFIBRATE ANALYSIS METHODS IN URINE (IN VITRO) USING SOLID PHASE EXTRACTION– HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-UV DETECTOR

Authors

  • Iyan Sopyan Departement of pharmaceutics and Technology of Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran
  • Widiastuty Irawati Depertemen of Pharmaceutical analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran.
  • Wiwiek Indriyati Depertemen of Pharmaceutical analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran.

DOI:

https://doi.org/10.22159/ijap.2018v10i1.22769

Keywords:

Bezafibrate, HPLC, SPE, Urine

Abstract

Objective: Bezafibrate is the second generation of fibrate groups used as the drug of choice in the treatment of hyperlipidemia. The purpose of this study is to obtained a validated method for analyzing bezafibrate in urine using solid phase extraction (SPE)-High performance liquid chromatography (HPLC).

Methods: Solid phase extraction (SPE) using hydrophilic-lipophilic balance (HLB) cartridge was performed for bezafibrate extraction from urine, afterward, a validation of analysis method using high-performance liquid chromatography (HPLC)-(UV) detection was conducted to parameters, including: selectivity (Rs), linearity (r), accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ).

Results: Recovery extraction using SPE resulted %recovery 85-110%. The analysis was performed by high-performance liquid chromatography using reversed phase, C18 octadecylsilane (ODS) columns 250 x 26 mm, particle size 10 μl, with the composition of 0.01 M acetate buffer with pH 3.55: with percent composition (45:55) and 0.8 ml/minute on 230 nm UV detection. Validation includes selectivity, linearity, accuracy, precision LOD, and LOQ have fulfilled requirement value.

Conclusion: The result of recovery extraction using SPE and validation of method exhibited the values that fulfilled the requirements and can be used for analysis bezafibrate in the urine.

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Author Biography

Iyan Sopyan, Departement of pharmaceutics and Technology of Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran

Dept. of pharmaceutics and technology of pharmacy

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Published

07-01-2018

How to Cite

Sopyan, I., Irawati, W., & Indriyati, W. (2018). A NOVEL OF BEZAFIBRATE ANALYSIS METHODS IN URINE (IN VITRO) USING SOLID PHASE EXTRACTION– HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-UV DETECTOR. International Journal of Applied Pharmaceutics, 10(1), 94–97. https://doi.org/10.22159/ijap.2018v10i1.22769

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