DEVELOPMENT OF SERUM WITH 4-N-BUTYLRESORSINOL IN THE TRANSETOSOMES VESICULAR SYSTEM

MEITI ROSMIATI; IYAN SOPYAN; ANIS YOHANA CHAERUNISAA; MARLINE ABDASSAH

doi:10.22159/ijap.2024v16i1.49328

Authors

MEITI ROSMIATI Department of Pharmaceuticals and Pharmaceutical Technology, Faculty of Pharmacy, Padjadjaran University, Indonesia. Department of Pharmaceuticals, Piksi Ganesha Politechnic, Indonesia
IYAN SOPYAN Department of Pharmaceuticals and Pharmaceutical Technology, Faculty of Pharmacy, Padjadjaran University, Indonesia https://orcid.org/0000-0001-7616-5176
ANIS YOHANA CHAERUNISAA Department of Pharmaceuticals and Pharmaceutical Technology, Faculty of Pharmacy, Padjadjaran University, Indonesia
MARLINE ABDASSAH Department of Pharmaceuticals and Pharmaceutical Technology, Faculty of Pharmacy, Padjadjaran University, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024v16i1.49328

Keywords:

4-n-Butylresorcinol (4nBR), Transethosomes, Box benhken approach, Penetration test

Abstract

Objective: The study aimed to develop a transetosome system as a delivery system of 4-n-Butylresorcinol (4nBR) and evaluate their physicochemical characteristics and skin penetration capacity compared with another vesicles system.

Methods: Transethosomes were prepared through cold methods and the optimization of the formulation was carried out using “Box–Behnken design” approach from Design-Expert software (version 13.0. 3. 0, State-Ease Inc., Minneapolis, MN). The independent variables were soya lecithin, surfactant (Tween 80: Span 80 with a ratio of 1: 3) and Ethanol. The prepared formulations were characterized for vesicle size, polydispersity (PDI), zeta potential using a particle size analyzer and entrapment efficiency. Furthermore, transethosomes were formulated in serum preparations that tested for in vitro penetration test compared to serum with ethosomes, transfersomes and non-vesicles system.

Results: Transethosomes formula optimization using box benhken approach produced a formula of 5.53 % soya lecithin, 3 % surfactant (Tween 80: Span 80 with a ratio of 1: 3) and 30 % Ethanol. The optimized formulation obtained particle size result of 197.4 nm; Polydispersity Index 0.421; zeta potential-56.8 mV and entrapment efficiency 98.40 %. Transethosomes serum met physical stability tests and in vitro penetration test showed better results compared to serum with ethosomes, transfersomes and non-vesicles system; the percentage of cumulative penetrated amounts of transethosomes serum, transfersomes, ethosomes and non-vesicle serum, respectively, was 41.43%; 23.59%, 19.85% and 2.43%.

Conclusion: Development of 4nBR transethosomes using surfactant as edge activators and ethanol as an enhancers through optimization with box Behnken design resulted in transethosomes composition as ultra-deformable vesicles that fulfiled the physical characteristics, stability and permeability of 4nBR.

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