DETERMINATION OF LEVELS OF GLUCOSAMINE HYDROCHLORIDE AND CHONDROITIN SULFATE IN MIXTURES IN TABLET AND CREAM FORMS USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE

Authors

  • Harmita Harmita Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
  • Catur Jatmika Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
  • Mufid Idan Nugraha Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.

DOI:

https://doi.org/10.22159/ijap.2017.v9s1.75_82

Keywords:

Chondroitin sulfate, Derivatization, Glucosamine hydrochloride, Fluorescence, High-performance liquid chromatography, Validation

Abstract

Objective: Glucosamine hydrochloride (HCl) and chondroitin sulfate are glycosaminoglycan compounds and major structural components of bones
in the form of proteoglycans. These compounds maintain bone structure by stimulating the synthesis of synovial fluid and inhibiting the degradation
of joint cartilage, and they can be used for the treatment of osteoarthritis. The aim of this study was to identify a selective analytical method for
determining glucosamine HCl and chondroitin sulfate levels in tablet and cream forms.
Methods: After derivatization using ortho-phthalaldehyde and 2-mercaptoethanol, the samples were analyzed using high-performance liquid
chromatography (HPLC) with a fluorescence detector at an excitation wavelength of 335 nm and an emission wavelength of 445 nm. Deacetylation
using sodium hydroxide was required to break the acetyl group bond. The mobile phase used tetrahydrofuran 0.25% in water-acetonitrile (87:13)
with a flow rate of 1.5 ml/minute.
Results: The average levels of glucosamine HCl and chondroitin sulfate were 92.76% and 96.11% in tablets and 101.15% and 100.33% in creams,
which fulfilled the acceptance criteria.
Conclusions: Our validation method for glucosamine HCl and chondroitin sulfate met the acceptance criteria of accuracy, precision, selectivity, and
linearity.

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References

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Table 13: Determination of glucosamine HCl levels in cream form

C (ppm) Standard area of glucosamine HCl Sample area of glucosamine HCl Measurable

concentration (ppm)

Measurable

concentration (%)

320059616 328605087 82.14 102.67

82.11 102.64

82.10 102.63

C: Concentration, HCL: Hydrochloride

Table 14: Determination of chondroitin sulfate levels in cream form

C (ppm) Standard area of chondroitin sulfate Sample area of chondroitin sulfate Measurable

concentration (ppm)

Measurable

concentration (%)

10382934 10431472 904.21 100.47

901.51 100.17

903.21 100.36

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Published

30-10-2017

How to Cite

Harmita, H., Jatmika, C., & Nugraha, M. I. (2017). DETERMINATION OF LEVELS OF GLUCOSAMINE HYDROCHLORIDE AND CHONDROITIN SULFATE IN MIXTURES IN TABLET AND CREAM FORMS USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE. International Journal of Applied Pharmaceutics, 9, 144–149. https://doi.org/10.22159/ijap.2017.v9s1.75_82

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