ANTIBACTERIAL EFFECT OF VIRGIN COCONUT OIL ON THE VIABILITY OF CHROMOGENIC BACTERIA THAT CAUSES DENTAL BLACK STAIN IN CHILDREN

Adita Gayatri; Eva Fauziah; Margaretha Suharsini

doi:10.22159/ijap.2017.v9s2.20

Authors

Adita Gayatri Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Eva Fauziah Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Margaretha Suharsini Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.22159/ijap.2017.v9s2.20

Keywords:

Chromogenic bacteria, Dental black stain, Cell viability, Virgin coconut oil

Abstract

Introduction: Virgin coconut oil (VCO) is a natural antibacterial agent with the ability to kill microorganisms whose cell membranes contain lipids, including Gram-positive and Gram-negative bacteria, such as Actinomyces sp. and Prevotella spp. No study on the antibacterial effects of VCO on the chromogenic bacteria that causes dental black stains.

Objective: To analyze the effects of VCO, which administered in various concentrations to Actinomyces sp. and Prevotella sp.

Methods: Actinomyces sp. and Prevotella sp. were isolated from the dental plaque of a child diagnosed with black stain. Each streak of bacteria was cultured on a selective medium and confirmed visually and through Gram staining. Each bacterial culture was exposed to VCO in concentrations of 12.5%, 25%, 50%, and 100%. Afterward, viability testing with a methyl-thiazolyl-tetrazolium assay was conducted, and the results were read using an Enzyme-linked immunosorbent assay reader.

Results: The administration of 12.5% VCO reduced bacterial viability; however, 25% VCO significantly reduced the viability of Actinomyces sp. and 100% VCO significantly reduced the viability of Prevotella sp.

Conclusion: Actinomyces sp. is more sensitive to VCO than Prevotella sp.

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