ANTIMICROBIAL POTENTIAL OF HYDROGEL INCORPORATED WITH PLGA NANOPARTICLES OF CROSSANDRA INFUNDIBULIFORMIS
DOI:
https://doi.org/10.22159/ijap.2019v11i2.31289Keywords:
Crossandra infundibuliformis, Nanoparticles, Hydrogel, Antimicrobial activityAbstract
Objective: Present study is aimed at formulation of Hydrogel containing Poly Lactic Glycolic Acid (PLGA) nanoparticles incorporated with ethanolic extract of Crossandra infundibuliformis (EECI) and investigate the efficacy of hydrogel nanoparticles as a carrier of antimicrobial constituents.
Methods: Poly Lactic Glycolic Acid (PLGA) nanoparticles containing ethanolic extract of Crossandra infundibuliformis (EECI) were synthesized by an emulsion-evaporation method and their physicochemical properties were studied. Polymeric PLGA nanoparticles were then incorporated into gel matrix, using Hydroxy Propyl Methyl Cellulose (HPMC K4M) as a base. The antibacterial activity of nanoparticulated hydrogel formulations were evaluated by agar well diffusion method against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa.
Results: Nanoparticulate hydrogel formulations exhibited high viscosity, neutral pH with good spreadability which is appropriate for transdermal application as well as showed prolonged drug release from optimized formulation up to 24 h. Nanoparticulate hydrogel formulations were effective inhibitors of all the micro-organisms with more promising activity against Staphylococcus aureus.
Conclusion: Nanoparticulate hydrogel formulation can be used as a feasible alternative to conventional formulations of Crossandra infundibuliformis extract with advanced permeation characteristics of antimicrobial constituents for transdermal application.
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References
Zhang L, Pornpattananangku D, Hu CMJ, Huang CM. Development of nanoparticles for antimicrobial drug delivery. Curr Med Chem 2010;17:585-94.
Dandekar PP, Jain R, Patil S, Dhumal R, Tiwari D, Sharma S, et al. Curcumin-loaded hydrogel nanoparticles: application in anti-malarial therapy and toxicological evaluation. J Pharm Sci 2010;9999:1–19.
Sharmila N, Gomathi N. Antibacterial, antioxidant activity and phytochemical studies of Crossandra infundibuliformis extract. Int J Phytomed 2011;3:151-6.
Elamathi R, Deepa T, Kavitha R, Kamalakannan P, Sridhar S, Kumar SJ. Phytochemical screening and antimicrobial activity of leaf extracts of Crossandra infundibuliformis L nees on common bacterial and fungal pathogens. Int J Curr Sci 2011;1:72-7.
Madhumitha G, Saral AM. Preliminary phytochemical analysis, antibacterial, antifungal and anticandidal activities of successive extracts of Crossandra infundibuliformis. Asian Pac J Trop Med 2011;4:192-5.
Madhumitha G, Saral AM. Screening of larvicidal activity of Crossandra infundibuliformis extract against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. Int J Pharm Pharm Sci 2012;4:485-7.
Vadivel E, Panwal SV. In vitro anticancer and insecticidal activity of Crossandra infundibuliformis. J Chem Pharm Res 2016;8:260-4.
Sumalatha K. Wound healing process and effect of petroleum ether extract of Crossandra infundibuliformis leaf extract in an excision wound model. Int J Preclinical Pharm Res 2012;3:42-9.
Nagavarma BVN, Yadav HKS, Vasudha AA, Shivakumar HG. Different techniques for preparation of the polymeric nanoparticles-a review. Asian J Pharm Clin Res 2012;5 Suppl 3:16-23.
Renugadevi K, Inbakandan D, Bavanilatha M, Poornima V. Cissus quadrangularis assisted biosynthesis of silver nanoparticles with antimicrobial and anticancer potentials. Int J Pharm Bio Sci 2012;3:437–45.
Ravi KV, Sevukarajan M, Vulava J, Pavankumar AG, Deepthi Y, Manjunath M, et al. Improvement of dissolution characteristics and bioavailability of Tadalafil by solid dispersion technique using water-soluble polymers. Int J Adv Pharm 2012;2:56-63.
James JP, Priya S, Jyothi D. Effect of PLGA polymer on antimicrobial activity and the release studies of nanoparticle hydrogel containing Mimosa pudica extract. Res J Pharm Tech 2018;11:2876-80.
Mishra US, Murthy PN, Pasa G, Kumar S. Formulation development and stan¬dardisation of herbal gel containing methanolic extract of Butea frondosa. Int Res J Pharm 2011;2:126-9.
Khan AW, Kotta S, Shahid Ansari SH, Sharma RK, Kumar A, Ali J. Formulation development, optimization and evaluation of Aloe vera gel for wound healing. Pharmacogn Mag 2013;9:1-22.
Basha BN, Prakasam K, Goli D. Formulation and evaluation of gel containing the fluconazole-antifungal agent. Int J Drug Dev Res 2011;3:109-28.
Sannino A, Demitri C, Madaghiele M. Biodegradable cellulose-based hydrogels: design and applications. Mater 2009;2:353–73.
Hamidi M, Azadi A, Rafiei P. Hydrogel nanoparticles in drug delivery. Adv Drug Delivery Rev 2008;60:1638–49.
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