ANALYSIS OF SYNTHETIC RED DYES IN RED SPINACH SAMPLES (AMARANTHUS TRICOLOR L.)
Keywords:Amaranth, Karmoisin, Paper chromatography, Ponceau 4R, Red spinach, Rhodamin B, Thin-layer chromatography-densitometry, thinlayer chromatography
Objective: The purpose of this study was to determine whether synthetic red dyes are present in red spinach samples.
Methods: The presence of the dyes Ponceau 4R, Carmoisine, Rhodamine B, Amaranth was determined in red spinach using reactions, followed by
paper chromatography and thin-layer chromatography (TLC)-densitometry. In paper chromatography analyses, analytes were eluted using n-butanolâ€“
ethanolâ€“water (4:5:5) and isobutanolâ€“ethanolâ€“water (3:2:4), and in TLC-densitometry, analytes were eluted with n-butanolâ€“ethanolâ€“water (3:7:1).
Results: No synthetic red dyes were found in the seven red spinach samples.
Conclusion: The synthetic dyes Rhodamin B, amaranth, Ponceau 4R, and Karmoisin were not found as contaminants of red spinach.
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Fig. 1: Differences of pH and temperature between experiments performed on different days
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