SYZYGIUM CUMINI (L.) SKEELS LEAF EXTRACT FRACTIONS AS ARGINASE INHIBITORS AND THE EFFECTS OF TANNINS ON THEIR ACTIVITY

ARI ARIEFAH HIDAYATI; BERNA ELYA; RANI SAURIASARI

doi:10.22159/ijap.2020.v12s1.FF059

Authors

ARI ARIEFAH HIDAYATI Graduate Program, Faculty of Pharmacy, Universitas Indonesia
BERNA ELYA Departement of Pharmacognosy-Pythochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, Jawa Barat 16424, Indonesia
RANI SAURIASARI 3Departement of Clinical Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Jawa Barat 16424, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020.v12s1.FF059

Keywords:

Arginase inhibitor, Ethyl acetate fraction, Methanol fraction, Syzygium cumini, Tannins

Abstract

Objective: Arginase inhibition could be a potential therapeutic approach for endothelial dysfunction. Syzygium cumini (L.) Skeels leaves contain
phenolic acids and flavonoids, which have been predicted to exhibit arginase inhibitory activity. Moreover, these leaves contain tannins, which can
form complexes with enzymes and lead to false-positive results during biological testing. Therefore, this study was conducted to evaluate the arginase
inhibitory activity of S. cumini leaf extract and fractions as well as to elucidate the effects of tannins on this activity.
Methods: S. cumini leaves were fractionated using n-hexane, ethyl acetate, and methanol. A colorimetric method was employed to evaluate arginase
inhibitory activity. Tannin elimination was performed through the gelatin precipitation method. Total phenolic and flavonoid contents of the fractions
were calculated using the Folin–Ciocalteu and aluminum chloride methods, respectively.
Results: Ethyl acetate and methanol fractions showed arginase inhibitory activity with half-maximal inhibitory concentrations (IC50) of 46.96 and
15.35 μg/mL, respectively. The methanol fraction was positive for tannins. After tannin elimination, this fraction exhibited less potent arginase
inhibitory activity, with an IC50 value of 53.03 μg/mL. The ethyl acetate fraction showed higher total phenolic and flavonoid contents than the methanol
fraction.
Conclusion: Tannins affected the arginase inhibitory activity of the methanol fraction of S. cumini leaves; however, the ethyl acetate fraction did not
contain tannins and could inhibit arginase activity.

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