DEVELOPMENT OF A HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR ANALYZING DISODIUM 5′-GUANYLATE AND DISODIUM 5′-INOSINATE LEVELS IN FLAVOR ENHANCERS

Authors

  • Dwi Karina Natalia Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Harmita . Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Taufiq Indra Rukmana Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.

DOI:

https://doi.org/10.22159/ijap.2018.v10s1.28

Keywords:

Nil, Nil, High-performance liquid chromatography, ion pair, Photodiode array

Abstract

Objective: This study aimed to develop a selective analytical method for assessing disodium 5′-guanylate and disodium 5′-inosinate levels in flavor
enhancers.
Methods: The levels were assessed using high-performance liquid chromatography (HPLC) with a photodiode array detector (PDA) (wavelength
=255 nm) and a SunFire® C18 column (250 mm × 4.6 mm × 5 μm). The mobile phase comprised a mixture of potassium phosphate buffer and an
ion pair reagent-hexane-1-sulfonic acid sodium salt - with a flow rate of 1.2 mL/min. The ion pair was used to generate a neutral equilibrium, which
resulted in increased retention of the analytes. Optimized analysis conditions were then validated regarding accuracy, precision, linearity, selectivity,
and the limits of detection and quantification.
Results: The average levels of disodium 5′-inosinate in the six analyzed samples were 0.24±1.46, 0.21±2.69, 0.58±3.26, 0.21±0.84, 0.22±3.59, and
0.47±2.21%, respectively. Regarding disodium 5′-guanylate, the average levels were 0.15±2.85, 0.15±0.12, 0.41±3.80, 0.16±1.72, 0.27±1.18, and
0.34±1.83, respectively.
Conclusion: The optimal conditions for analyzing disodium 5′-guanylate and disodium 5′-inosinateusing HPLC with a PDA and SunFire C18 column
were λ=255 nm, a mobile phase of potassium phosphate buffer and sodium hexane sulfonate, and a flow rate of 1.2 mL/min. For disodium 5′-inosinate,
its average levels in samples A–F were 0.24±1.46, 0.21±2.69, 0.58±3.26, 0.21±0.84, 0.22±3.59, and 0.47±2.21%, respectively. Meanwhile, the average
levels of disodium 5′-guanylate in the samples were 0.15±2.85, 0.15±0.12, 0.41±3.80, 0.16±1.72, 0.27±1.18, and 0.34±1.83%, respectively.

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References

Irdasari N. Analysis of Consumer Attitudes Toward the Performance of

the Masako to Enrich the Flavor. Thesis. Bogor: Faculty of Agriculture,

Intitut Pertanian Bogor; 2009.

Qiu W, Chen S, Xie J, Qu Y, Song X. Analysis of 10 nucleotides and

related compounds in Litopenaeus vannamei during chilled storage by

HPLC-DAD. LWT Food Sci Technol 2016;67:187-93.

Kojima K. Safety evaluation of disodium 5’-inosinate, disodium

’-guanylate and disodium 5’-ribonucleotide. Toxicology 1974;2:185‑206.

Ekelman K, Raffaele C. 788. Disodium 5’-guanylate and disodium

’-inosinate. Geneva: WHO Food Additives Series 32, Inchem.org.; 2008.

Viñas P, Campillo N, Melgarejo GF, Vasallo MI, López-García I, Hernández-

Córdoba M, et al. Ion-pair high-performance liquid chromatography with

diode array detection coupled to dual electrospray atmospheric pressure

chemical ionization time-of-flight mass spectrometry for the determination

of nucleotides in baby foods. J Chromatogr A 2010;1217:5197-203.

Yang FQ, Li DQ, Feng K, Hu DJ, Li SP. Determination of nucleotides,

nucleosides and their transformation products in cordyceps by ionpairing

reversed-phase liquid chromatography-mass spectrometry.

J Chromatogr A 2010;1217:5501-10.

Goday S, Abdul RS, Prameelarani A. Development of a new stability

indicating RP-HPLC method for simultaneous estimation of epalrestat

and pregabalin and its validation as per international conference on

harmonization guidelines. Asian J Pharm Clin Res 2018;11:319-24.

Senthilraja M, Giriraj P. Reverse phase HPLC method for the

simultaneous estimation of terbutanile sulphate, bromhexine HCL and

guaifenesin in cough syrup. Asian J Pharm Clin Res 2011;4:13-5.

Vilas BW, Tushar AD, Vijay RP. Development and validation of RPHPLC

method for estimation of diosgenin in pharmaceutical dosage

form. Asian J Pharm Clin Res 2011;4:126-8.

John LD, Lloyd RS, Joseph JK. Introduction to Modern Liquid

Chromatography. 3rd ed. Hoboken, New Jersey: Wiley; 2013.

Yuri K, Rosario L. HPLC for Pharmaceutical Scientists. Hoboken, New

Jersey: Wiley Interscience; 2007.

Published

20-12-2018

How to Cite

Natalia, D. K., ., H., & Rukmana, T. I. (2018). DEVELOPMENT OF A HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR ANALYZING DISODIUM 5′-GUANYLATE AND DISODIUM 5′-INOSINATE LEVELS IN FLAVOR ENHANCERS. International Journal of Applied Pharmaceutics, 10(1), 133–137. https://doi.org/10.22159/ijap.2018.v10s1.28

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