ANTIMICROBIAL ACTIVITY OF MINERAL TRIOXIDE AGGREGATE AND CALCIUM HYDROXIDE SEALER ON ENTEROCOCCUS FAECALIS STRAIN ATCC29212
DOI:
https://doi.org/10.22159/ijap.2019.v11s1.AR178Keywords:
Antimicrobial activity, Calcium hydroxide sealer, Enterococcus faecalis, Mineral trioxide aggregate sealerAbstract
Objective: The objective of this study was to evaluate the antimicrobial activity of mineral trioxide aggregate (MTA) and calcium hydroxide (Ca(OH)2)
sealers on Enterococcus faecalis at various time points after the preparation of the sealer.
Methods: Antimicrobial activity of MTA and Ca(OH)2 sealer on E. faecalis (ATCC 29212) was assessed by the direct contact test immediately, 1 day,
and 7 days after the preparation of the sealer.
Results: The antimicrobial effect of MTA sealer was greatest 1 and 7 days after the preparation of the sealer. The effect of Ca(OH)2 was greatest 1 day
after the preparation of the sealer.
Conclusion: MTA sealer showed constant antimicrobial activity on E. faecalis overtime. By contrast, Ca(OH)2 sealer showed decreasing antimicrobial
activity overtime, with the greatest antimicrobial activity being 1 day after the preparation of the sealer.
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References
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hydroxide inactivates lipoteichoic acid from Enterococcus faecalis
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hydroxide associated with different substances against Enterococcus
faecalis strains. Dent Press Endod 2011;1:46-51.
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zoolu-website/media/document/1207/Apexit+Plus. [Last accessed on
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29. Zhang H, Shen Y, Ruse ND, Haapasalo M. Antibacterial activity of
endodontic sealers by modified direct contact test against Enterococcus
faecalis. J Endod 2009;35:1051-5.
30. Slutzky-Goldberg I, Slutzky H, Solomonov M, Moshonov J, Weiss EI,
Matalon S, et al. Antibacterial properties of four endodontic sealers.
J Endod 2008;34:735-8.
Relationship to endodontic disease. Crit Rev Oral Biol Med
2004;15:308-20.
2. Johnson WT, Kulild JC. Obturation of the cleaned and shaped root
canal system. In: Hargreaves KM, Cohen S, editors. Cohen’s Pathways
of the Pulp. 10th ed. St Louis: Mosby Elsevier; 2011. p. 349, 359-61.
3. American Association of Endodontics: Endodontics Colleagues
for Excellence. Obturation of Root Canal Systems 2009. Available
from: http://www.aae.org/uploadedFiles/Publications_and_Research/
Endodontics_Colleagues_for_Excellence_Newsletter/fall09ecfe.pdf,
tanggal. [Last accessed on 2013 Jul 25].
4. Rawtiya M, Verma K, Singh S, Munuga S, Khan S. MTA-based root
canal sealers. J Orofac Res 2013;3:16-21.
5. Bouillaguet S, Wataha JC, Lockwood PE, Galgano C, Golay A, Krejci I,
et al. Cytotoxicity and sealing properties of four classes of endodontic
sealers evaluated by succinic dehydrogenase activity and confocal laser
scanning microscopy. Eur J Oral Sci 2004;112:182-7.
6. Rao A, Rao A, Shenoy R. Mineral trioxide aggregate - A review. J Clin
Pediatr Dent 2009;34:1-8.
7. Goel M, Bala S, Sachdeva G, Shweta C. Comparative ecaluation of mta,
calcium hydroxide and Portland cement as a root end filling materials:
A comprehensive review. Indian J Dent Sci 2011;5:130-3.
8. Harshard PN, Wasim M. Evaluation of the effect of ph in the elimination
of Enterococcus faecalis-in vitro study. J Ahmedabad Dent Coll Hosp
2011;2:119-123.
9. Mohammadi Z, Dummer PM. Properties and applications of calcium
hydroxide in endodontics and dental traumatology. Int Endod J
2011;44:697-730.
10. Gopikrishna V, Kandaswamy D, Jeyavel RK. Comparative evaluation
of the antimicrobial efficacy of five endodontic root canal sealers
against Enterococcus faecalis and candida albicans. J Conserv Dent
2006;9:2-12.
11. Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus
faecalis: Its role in root canal treatment failure and current concepts in
retreatment. J Endod 2006;32:93-8.
12. Chai WL, Hamimah H, Cheng SC, Sallam AA, Abdullah M.
Susceptibility of Enterococcus faecalis biofilm to antibiotics and
calcium hydroxide. J Oral Sci 2007;49:161-6.
13. Athanassiadis B, Abbott PV, Walsh LJ. The use of calcium hydroxide,
antibiotics and biocides as antimicrobial medicaments in endodontics.
Aust Dent J 2007;52:S64-82.
14. Desai S, Chandler N. Calcium hydroxide-based root canal sealers:
A review. J Endod 2009;35:475-80.
15. Rawtiya M, Verma K, Singh S, Munuga S, Khan S. MTA-based root
canal sealers. J Orofac Res 2013;3:16-21.
16. MTA Fillapex Endodontic Sealer. Available from: https://www.
clinicalresearchdental.com/marketing/mta%20fillapex%20-%20
scientific%20profile_medium.pdf. [Last accessed on 2013 May 15].
17. Chang SW. Chemical characteristics of mineral trioxide aggregate and
its hydration reaction. Restor Dent Endod 2012;37:188-93.
18. Gomes-Filho JE, Moreira JV, Watanabe S, Lodi CS, Cintra LT, Dezan
Junior E, et al. Sealability of MTA and calcium hydroxidecontaining
sealers. J Appl Oral Sci 2012;20:347-51.
19. Siqueira JF Jr., Lopes HP. Mechanisms of antimicrobial activity of
calcium hydroxide: A critical review. Int Endod J 1999;32:361-9.
20. Ustun Y, Sagsen B, Durmaz S, Percin D. In vitro antimicrobial
efficiency of different root canal sealers against Enterecoccus faecalis.
Eur J Gen Dent 2013;2:134-8.
21. Al-Hezaimi K, Al-Shalan TA, Naghshbandi J, Oglesby S, Simon JH,
Rotstein I, et al. Antibacterial effect of two mineral trioxide aggregate
(MTA) preparations against Enterococcus faecalis and Streptococcus
sanguis in vitro. J Endod 2006;32:1053-6.
22. Sluyk SR, Moon PC, Hartwell GR. Evaluation of setting properties and
retention characteristics of mineral trioxide aggregate when used as a
furcation perforation repair material. J Endod 1998;24:768-71.
23. Stowe TJ, Sedgley CM, Stowe B, Fenno JC. The effects of chlorhexidine
gluconate (0.12%) on the antimicrobial properties of tooth-colored
proRoot mineral trioxide aggregate. J Endod 2004;30:429-31.
24. Parirokh M, Torabinejad M. Mineral trioxide aggregate:
A comprehensive literature review - part I: Chemical, physical, and
antibacterial properties. J Endod 2010;36:16-27.
25. Baik JE, Jang KS, Kang SS, Yun CH, Lee K, Kim BG, et al. Calcium
hydroxide inactivates lipoteichoic acid from Enterococcus faecalis
through deacylation of the lipid moiety. J Endod 2011;37:191-6.
26. Vitti RP, Prati C, Silva EJ, Sinhoreti MA, Zanchi CH, de Souza
e Silva MG, et al. Physical properties of MTA fillapex sealer. J Endod
2013;39:915-8.
27. Weckwerth PH, Siquinelli NB, Weckwerth AC, Vivan RR, Duarte MA.
In vitro determination of direct antimicrobial effect of calcium
hydroxide associated with different substances against Enterococcus
faecalis strains. Dent Press Endod 2011;1:46-51.
28. Sbicego S. Apexit Plus Scientific Documentation. Liechtenstein: Ivoclar
Vivadent AG; 2007. Available from: http://www.ivoclarvivadent.com/
zoolu-website/media/document/1207/Apexit+Plus. [Last accessed on
2013 Agu 20].
29. Zhang H, Shen Y, Ruse ND, Haapasalo M. Antibacterial activity of
endodontic sealers by modified direct contact test against Enterococcus
faecalis. J Endod 2009;35:1051-5.
30. Slutzky-Goldberg I, Slutzky H, Solomonov M, Moshonov J, Weiss EI,
Matalon S, et al. Antibacterial properties of four endodontic sealers.
J Endod 2008;34:735-8.
Published
05-04-2019
How to Cite
PRATHITA, T., DJAUHARIE, N. K., & MEIDYAWATI, R. (2019). ANTIMICROBIAL ACTIVITY OF MINERAL TRIOXIDE AGGREGATE AND CALCIUM HYDROXIDE SEALER ON ENTEROCOCCUS FAECALIS STRAIN ATCC29212. International Journal of Applied Pharmaceutics, 11(1), 123–125. https://doi.org/10.22159/ijap.2019.v11s1.AR178
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