COMPARISON OF HUMAN PLATELET LYSATE AND FETAL BOVINE SERUM IN CULTURE MEDIA FOR HUMAN DENTAL PULP STEM CELL PROLIFERATION

Authors

  • DINI ASRIANTI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • ANGGRAINI MARGONO Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • SILVIANA SWASTININGTYAS Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • ILMILDA SANDY RATNA ASRI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • MUNYATI USMAN Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • INDAH YULIANTO Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.22159/ijap.2019.v11s1.16025

Keywords:

Human platelet lysate, Fetal bovine serum, Human dental pulp stem cells proliferation

Abstract

Objective: Ex vivo and in vitro cell cultures require a basal medium with added supplements containing growth factors, proteins, and enzymes to
support attachment, growth, and proliferation. Fetal bovine serum (FBS) is used to supplement cell culture media. However, human platelet lysate
(hPL) represents an attractive alternative as it is nonxenogeneic.
Methods: Human third molars were collected from six healthy donors (19–35 years old) with no history of regular alcohol consumption or smoking.
Human dental pulp stem cells (hDPSCs) at the second passage were divided into two culture media groups, 10% FBS and 5% hPL, as well as a control
group after 24 h of serum starvation. A flow cytometry analysis was conducted to measure CD90, CD105, CD73, CD34, CD45, and Human Leukocyte
Antigen-DR isotype (HLA-DR). Cellular proliferation was evaluated on days 1, 3, and 5.
Results: The flow cytometry analysis revealed that the majority of the cells expressed positive mesenchymal stem cell surface markers, including
CD73 (98.5%), CD90 (98.3%), and CD105 (71.0%), and lacked CD34, CD45, and HLA-DR. There were significant differences among the 5% hPL, 10%
FBS, and control groups on days 1, 3, and 5.
Conclusion: For a nonxenogeneic culture, 5% hPL can be used as an alternative in culture media for hDPSC proliferation.

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References

1. Diogenes A, Ruparel NB, Shiloah Y, Hargreaves KM. Regenerative
endodontics a way forward. J Am Dent Assoc 2016;147:372-80.
2. Torabinejad M, Nosrat A, Verma P, Udochukwu O. Regenerative
endodontic treatment or mineral trioxide aggregate apical plug in teeth
with necrotic pulps and open apices: A Systematic review and metaanalysis.
J Endod 2017;43:1806-20.
3. Kim SG, Malek M, Sigurdsson A, Lin LM, Kahler B. Regenerative
endodontics: A comprehensive review. Int Endod J 2018;51:1367-88.
4. Sreedev CP, Karthick K, Mathew S, Raju I. Regenerative endodontics:
An overview. J Indian Acad Dent Spec Res 2017;4:18-22.
5. Mookhtiar H, Hegde V, Shanmugsundaram S. A new innovation in
dentistry: Regenerative endodonics. Int J Rec Sci Res 2018;9:24881-7.
6. Chen B, Sun HH, Wang HG, Kong H, Chen FM, Yu Q, et al. The
effects of human platelet lysate on dental pulp stem cells derived from
impacted human third molars. Biomaterials 2012;33:5023-35.
7. Bernardo ME, Avanzini MA, Perotti C, Cometa AM, Moretta A, Lenta E,
et al. Optimization of Io expansion of human multipotent mesenchymal
stromal cells for cell-therapy approaches: Further insights in the search
for a fetal calf serum substitute. J Cell Physiol 2007;211:121-30.
8. Govindasamy V, Ronald VS, Abdullah AN, Ganesan Nathan KR,
Aziz ZA, Abdullah M, et al. Human platelet lysate permits scale-up
of dental pulp stromal cells for clinical applications. Cytotherapy
2011;13:1221-33.
9. Hemeda H, Giebel B, Wagner W. Evaluation of human platelet lysate
versus fetal bovine serum for culture of mesenchymal stromal cells. Int
Soc Cell Ther 2014;16:170-80.
10. Bieback K. Platelet lysate as replacement for fetal bovine serum
in mesenchymal stromal cell cultures. Transfus Med Hemother
2013;40:326-35.
11. Saeed MA, El-Rahman MA, Helal ME, Zaher AR, Grawish ME.
Efficacy of human platelet rich fibrin exudate vs fetal bovine serum
on proliferation and differentiation of dental pulp stem cells. Int J Stem
Cells 2017;10:38-47.
12. Griffiths S, Baraniak PR, Copland IANB, Nerem RM, Mcdevitt TC.
Human platelet lysate stimulates high-passage and senescent human
multipotent mesenchymal stromal cell growth and rejuvenation in vitro.
J Cytotherapy 2013;15:1469-83.
13. Biomedical Corporation. UltraGROTM Product Description.
Alpharetta: Biomedical Corporation; 2015. p. 1-2.
14. Marsa RD, Asrianti D, Margono A. The efficacy of platelet-rich fibrin
lysate (PRF-L) for fibroblast cell proliferation. J Int Dent Med Res
2017;10:809-13.
15. Ben Azouna N, Jenhani F, Regaya Z, Berraeis L, Ben Othman T,
Ducrocq E, et al. Phenotypical and functional characteristics of
mesenchymal stem cells from bone marrow: Comparison of culture
using different media supplemented with human platelet lysate or fetal
bovine serum. Stem Cell Res Ther 2012;3:6.
16. Marrazzo P, Paduano F, Palmieri F, Marrelli M, Tatullo M. Highly
efficient in vitro reparative behaviour of dental pulp stem cells cultured
with standardised platelet lysate supplementation. Stem Cells Int
2016;2016:7230987.
17. Ma Y, Reilly S, Fischer S, Henshaw M, Pierce J, Poudel A, et al. Human
platelet lysate produced at industrial scale for use as fetal bovine serum
replacement in cell manufacturing protocols. Cytotherapy 2015;17:S41.
18. Masuki H, Okudera T, Watanebe T, Suzuki M, Nishiyama K, Okudera H,
et al. Growth factor and pro-inflammatory cytokine contents in plateletrich
plasma (PRP), plasma rich in growth factors (PRGF), advanced
platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF).
Int J Implant Dent 2016;2:19.

Published

05-04-2019

How to Cite

ASRIANTI, D. ., MARGONO, A. ., SWASTININGTYAS, S. ., ASRI, I. S. R. ., USMAN, M. ., & YULIANTO, I. (2019). COMPARISON OF HUMAN PLATELET LYSATE AND FETAL BOVINE SERUM IN CULTURE MEDIA FOR HUMAN DENTAL PULP STEM CELL PROLIFERATION. International Journal of Applied Pharmaceutics, 11(1), 157–159. https://doi.org/10.22159/ijap.2019.v11s1.16025

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