TRANSFORMING GROWTH FACTOR-β1 EXPRESSION IN VARIOUS CONCENTRATIONS OF ADVANCED PLATELET-RICH FIBRIN MODULATING HUMAN DENTAL PULP STEM CELL DIFFERENTIATION
DOI:
https://doi.org/10.22159/ijap.2020.v12s2.OP-44Keywords:
Advanced platelet-rich fibrin, Transforming growth factor beta 1, Differentiation, Human dental pulp stem cellsAbstract
Objective: Modification of the speed and time of centrifugation based on the low-speed centrifugation concept for platelet-rich fibrin (PRF) has resulted in a new type of PRF known as advanced PRF (A-PRF). A-PRF can release several types of growth factors (GFs) that participate in the process of differentiation, such as transforming GF-β1 (TGF-β1). The aim of this study was to analyze TGF-β1 expression in various concentrations of A-PRF in the differentiation process of human dental pulp stem cells (hDPSCs).
Methods: hDPSC cultures were obtained from those of previous research (ethical approval form has been attached). These hDPSCs were in the 2nd–3rd passage, and serum starvation was done by reducing fetal bovine serum (FBS) levels in the hDPSC culture media. A-PRF was obtained using 10 ml blood collected from the cubital vein, which was centrifuged at 1500 rpm for 14 min and then divided into four concentration groups. TGF-β1 expression in 1%, 5%, and 25% A-PRF as well as in 10% FBS (control) was analyzed by ELISA on day 7.
Results: Although no significant differences were observed in TGF-β1 expression between 1%, 5%, and 25% A-PRF, and 10% FBS, it was observed that the higher the concentration of A-PRF, the greater the TGF-β1 expression.
Conclusion: The expression of TGF-β1 was consistent with the increase in A-PRF concentration. The highest TGF-β1 expression was detected in 25% A-PRF among all concentrations in the differentiation process of hDPSCs.
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References
Hong S, Chen BJ. A comparative evaluation of concentrated growth
factor and platelet-rich fibrin on the proliferation, migration, and
differentiation of human stem cells of the apical papilla. J Endod
;1:977-83.
Srivastava S. Current and future perspectives for dentin-pulp tissue
engineering-an update. SADJ 2019;74:110-4.
Mari-Beffa M, Segura-Egea JJ, Diaz-Cuenca A. Regenerative
endodontic procedures: A perspective from stem cell niche biology. J
Endod 2017;43:52-62.
El Bagdadi K, Kubesch A, Yu X, Al-Maawi S, Orlowska A, Dias A,
et al. Reduction of relative centrifugal forces increases growth factor
release within solid platelet-rich-fibrin (PRF)-based matrices: A proof
of concept of LSCC (low speed centrifugation concept). Eur J Trauma
Emerg Surg 2019;45:467-79.
Bagio DA, Julianto I, Suprastiwi E, Margono A. Ideal concentration
of advanced-platelet rich fibrin (A-PRF) conditioned media for human
dental pulp stem cells differentiation. APESB 2019;19:4754.
Niwa T, Yamakoshi Y, Yamazaki H, Karakida T, Chiba R, Hu JC, et al.
The dynamics of TGF-β in dental pulp, odontoblasts and dentin. Sci
Rep 2018;8:4450.
da Rosa WL, Piva E, da Silva AF. Disclosing the physiology of dental
pulp for vital pulp therapy. Int Endod J 2018;51:829-46.
Marsa RD, Asrianti D, Margono A. The efficacy of platelet-rich fibrin
lysate (PRF-L) for fibroblast cell proliferation. J Int Dent Med Res
;10:809-13.
He L, Zhong J, Gong Q. Regenerative endodontics by cell homing.
Dent Clin N Am 2017;61:143-59.
Lin PS, Chang HH, Yeh CY, Chang MC, Chan CP, Kuo HY, et al.
Transforming growth factor Beta 1 increases collagen content, and
stimulates procollagen I and tissue inhibitor of metalloproteinase-1
production of dental pulp cells: Role of MEK/ERK and activin receptorlike
kinase-5/smad signaling. J Formos Med Assoc 2017;116:351-8.
Orti V, Collart-Dutilleul PY, Piglionico S, Pall O, Cuisinier F, Panayotov I.
Pulp regeneration concepts for non-vital teeth: From tissue engineering to
clinical approaches. Tissue Eng Part B Rev 2018;24:419-42.
Maniyar N, Sarode GS, Sarode SC, Shah J. Platelet-rich fibrin: A
“wonder material” in advanced surgical dentistry. Med J D Y Patil
Vidyapeeth 2018;11:287.
Margono A, Asrianti D, Meydiawati R. The efficacy of advanced
platelet-rich fibrin (A-PRF) on fibroblast cell regeneration. J Int Dent
Med Res 2017;10:789-92.
Berridge M. Cell Cycle and Proliferation. United Kingdom: Portland
Press Limited; 2014.
Berridge MJ. Module 8: Development in cell signaling biology.
Portland Press Limited 2014; 8:1-61.
Grafe I, Alexander S, Peterson JR, Snider TN, Levi B, Lee B, et al.
TGF-b family signaling in mesenchymal differentiation. Cold Spring
Harb Perspect Bio 2018;10:a022202.
Morikawa M, Derynck R, Miyazono K. TGF-b and the TGF-b family:
Context-dependent roles in cell and tissue physiology. Cold Spring
Harb Perspect Biol 2016;8:a021873.
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