ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITY OF HIBISCUS ACETOSELLA LEAVES EXTRACTS

THISAKORN DUMRONGPHUTTIDECHA; SURADWADEE THUNGMUNGMEE; WARACHATE KHOBJAI; NAKUNTWALAI WISIDSRI; SURACHAI TECHAEOI

doi:10.22159/ijap.2019.v11s5.T0057

Authors

THISAKORN DUMRONGPHUTTIDECHA Department of Thai Traditional Medicine, Rajamangala University of Technology Thanyaburi, Pathumthani, Thailand.
SURADWADEE THUNGMUNGMEE Department of Thai Traditional Medicine, Rajamangala University of Technology Thanyaburi, Pathumthani, Thailand.
WARACHATE KHOBJAI Department of Thai Traditional Medicine, Rajamangala University of Technology Thanyaburi, Pathumthani, Thailand
NAKUNTWALAI WISIDSRI Department of Thai Traditional Medicine, Rajamangala University of Technology Thanyaburi, Pathumthani, Thailand.
SURACHAI TECHAEOI Department of Thai Traditional Medicine, Rajamangala University of Technology Thanyaburi, Pathumthani, Thailand

DOI:

https://doi.org/10.22159/ijap.2019.v11s5.T0057

Keywords:

Hibiscus acetosella, Antioxidants, Radical scavenging activity, Nitric oxide radical

Abstract

Objective: Hibiscus acetosella (HA) or Chaba Maple is native plant and cultivated in tropical western in Africa and north of America. The characteristics
of HA are red to purple in stem, leaf, and flower that are the pigment of antioxidant compound as anthocyanins. Anthocyanins are in the group
of flavonoid and have the role as functional foods which have several health benefits such as obesity and diabetes control, cardiovascular disease
prevention, and others. Hence, the aim of this study was to investigate the antioxidant and free radical scavenging activity of HA leaves extracts.
Materials and Methods: HA (Chaba Maple) leaves were collected in Pathum Thani province, Thailand, and were dried and extracted by maceration
technique with three solvents – water, ethanol, and methanol. The antioxidant properties of extracts were carried out using 2,2’-azino-bis(3-
ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay. The extracts were
examined for their scavenging effect on hydroxyl radical (•OH) using hydroxyl radical scavenging assay and nitric oxide (NO) radical (•NO) using NO
radical scavenging assay.
Results: For ABTS, FRAP, and hydroxyl radical scavenging assay, ethanol extract showed the highest antioxidant property which the percentage
inhibitions were 69.04%, 2381.84 μM/mg extract, and 62.88 mg/ml, respectively. For NO scavenging activity, methanol extract showed highest ability
to scavenge NO which percentage inhibition was 101.28±0.73 mg/ml.
Conclusion: The results of this study showed that ethanolic, methanolic, and water extract of HA leaves had scavenge and reducing antioxidant
properties.

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References

1. Tosato M, Zamboni V, Ferrini A, Cesari M. The aging process and
potential interventions to extend life expectancy. Clin Interv Aging
2007;2:401-12.
2. Jin K. Modern biological theories of aging. Aging Dis 2010;1:72-4.
3. Viña J, Borrás C, Miquel J. Theories of ageing. IUBMB Life
2007;59:249-54.
4. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants
and functional foods: Impact on human health. Pharmacogn Rev
2010;4:118-26.
5. Gutowski M, Kowalczyk S. A study of free radical chemistry: Their role
and pathophysiological significance. Acta Biochim Pol 2013;65:341-9.
6. Boora F, Chrisa E, Mukanganyama S. Evaluation of nitrite radical
scavenging properties of selected Zimbabwean plant extracts and their
phytoconstituents. J Food Process 2014;2014:7.
7. Sakhanokho HF, Kelley RY. Influence of salicylic acid on in vitro
propagation and salt tolerance in Hibiscus acetosella and Hibiscus
moscheutos (cv ‘Luna Red’). Afr J Biotechnol 2009;8:1474-81.
8. Duymu? HG, Göger F, Ba?er KH. In vitro antioxidant properties
and anthocyanin compositions of elderberry extracts. Food Chem
2014;155:112-9.
9. Kruger MJ, Davies N, Myburgh KH, Lecour S. Proanthocyanidins,
anthocyanins and cardiovascular diseases. Food Res Int 2014;59:41-52.
10. Siddhuraju P. The antioxidant activity and free radical-scavenging
capacity of phenolics of raw and dry heated moth bean (Vigna
aconitifolia) (Jacq) marachal seed extracts. Food Chem 2006;99:149-57.
11. Lipinski B. Hydroxyl radical and its scavengers in health and disease.
Oxid Med Cell Longev 2011;2011:9.
12. Rabeta MS, Faraniza RN. Total phenolic content and ferric reducing
antioxidant power of the leaves and fruits of Garcinia atroviridis and
Cynometra cauliflora. Int Food Res J 2013;20:1691-6.
13. Pavithra K, Vadivukkarasi S. Evaluation of free radical scavenging
activity of various extracts of leaves from Kedrostis foetidissima (Jacq.)
Cogn. Food Sci Human Wellness 2015;4:42-6.
14. Shalaby EA, Shanab SM. Comparison of DPPH and ABTS assays for
determining antioxidant potential of water and methanol extracts of
Spirulina platensis. Indian J Geo Mar Sci 2013;42:556-64.
15. Mitic MN, Kostic DA, Pavlovic AN, Dimitrijevic DS, Veljkovic JN.
Effects of solvent extraction system on concentration and antioxidant
activity of strawberry phenolics. Agro Food Ind Hi Tech 2014;25:24-9.
16. Sowndhararajan K, Kang SC. Free radical scavenging activity from
different extracts of leaves of Bauhinia vahlii Wight and arn. Saudi J
Biol Sci 2013;20:319-25.
17. Rajurkar NS, Hande SM. Estimation of phytochemical content and
antioxidant activity of some selected traditional Indian medical plants.
Indian J Pharm Sci 2011;73:146-51.
18. Khoo HE, Azlan A, Tang ST, Lim SM. Anthocyanidins and
anthocyanins: Colored pigments as food, pharmaceutical ingredients,
and the potential health benefits. Food Nutr Res 2017;61:1361779.
19. Anusuya N, Anusuya S, Manian R, Siddhuraju P, Manian S. Antioxidant
and free radical scavenging activity of certain dietary and medicinal
plant extracts. Glob Sci Books 2009;3:47-52.
20. Luiking YC, Engelen MP, Deutz NE. Regulation of nitric oxide
production in health and disease. Curr Opin Clin Nutr Metab Care
2010;13:97-104.
21. Gheddouchi S, Mokhtari-Soulimane N, Merzouk H, Bekhti F,
Soulimane F, Guermouche B, et al. Low SOD activity is associated
with overproduction of peroxynitrite and nitric oxide in patients with
acute coronary syndrome. Nitric Oxide 2015;49:40-6.
22. Ketnawa S, Ogawa Y. Evaluation of protein digestibility of fermented
soybeans and changes in biochemical characteristics of digested
fractions. J Funct Foods 2019;52:640-7.
23. Vilela TC, Leffa DD, Damiani AP, Damazio DD, Manenti AV,
Carvalho TJG, et al. Hibiscus acetosella extract protects against
alkylating agent-induced DNA damage in mice. An Acad Bras Cienc
2018;90:3165-74.