EVALUATION OF ACETYLCHOLINESTERASE ACTIVITY AND CYTOTOXICITY OF DIFFERENT PARTS OF NELUMBO NUCIFERA GAERTN ON HUMAN NEUROBLASTOMA CELL LINE (SH-SY5Y)
DOI:
https://doi.org/10.22159/ijap.2019.v11s5.T0080Keywords:
Nelumbo nucifera Gaertn, Acetylcholinesterase activity, Human neuroblastoma cell lineAbstract
Objective: Cognitive deterioration occurring in patients with probable Alzheimer’s disease is associated with a progressive loss of cholinergic neurons
and a consequent decline in levels of acetylcholine in the brain. This study aimed to evaluate the acetylcholinesterase (AChE) inhibitory effects and
cytotoxicity in SH-SY5Y cells of different parts of three lotus extracts.
Methods: AChE activity was quantified by spectrophotometry and cytotoxicity by flow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide
assay in SH-SY5Y cells exposed to extracts.
Results: All of the extracts had inhibitory effects to AChE at p<0.05, but Roseum Plenum stem extract could inhibit AChE more than 30% (p<0.05). The
all of the extracts could be an increase SH-SY5Y cell proliferation, while Album Plenum flower extract could be cytotoxic on SH-SY5Y cells.
Conclusion: The extracts of lotus could be supplemented compound for cognitive deterioration or Alzheimer’s patients.
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activity of different parts of lotus (Nelumbo nucifera Gaertn) rhizome.
Food Sci Technol (Campanas) 2017;37:135-8.
24. Liu CP, Tsai WJ, Lin YL, Liao JF, Chen CF, Kuo YC, et al. The extracts
from Nelumbo nucifera suppress cell cycle progression, cytokine
genes expression, and cell proliferation in human peripheral blood
mononuclear cells. Life Sci 2004;75:699-716.
25. Hu M, Skibsted LH. Antioxidative capacity of rhizome extract and
rhizome knot extract of edible lotus (Nelumbo nucifera). Food Chem
2002;76:327-33.
26. Wang QZ, Pen GH, Jin Y, Li J, Yan SL. Extraction of polyphenol
from lotus roots and its enzymatic browning substrate. J Anal Sci
2004;20:38?40.
27. Sridhar KR, Rajeev B. Lotus a potential nutraceutical source. J Agric
Technol 2007;3:143-55.
28. Ellman GL, Courtney KD, Andres V Jr., Feather-Stone RM. A new
and rapid colorimetric determination of acetylcholinesterase activity.
Biochem Pharmacol 1961;7:88-95.
29. Santillo MF, Liu Y. A fluorescence assay for measuring
acetylcholinesterase activity in rat blood and a human neuroblastoma
cell line (SH-SY5Y). J Pharmacol Toxicol Methods 2015;76:15-22.
30. Sun W, Chen L, Zheng W, Wei X, Wu W, Duysen EG, et al. Study of
acetylcholinesterase activity and apoptosis in SH-SY5Y cells and mice
exposed to ethanol. Toxicology 2017;384:33-9.
in the United States and the public health impact of delaying disease
onset. Am J Public Health 1998;88:1337-42.
2. Ellis JM. Cholinesterase inhibitors in the treatment of dementia. J Am
Osteopath Assoc 2005;105:145-58.
3. Poirier J. Evidence that the clinical effects of cholinesterase inhibitors
are related to potency and targeting of action. Int J Clin Pract
Suppl 2002;127:6-19.
4. Patestas MA, Gartner LP. A Textbook of Neuroanatomy. New York:
John Wiley and Sons; 2016.
5. Mesulam M, Guillozet A, Shaw P, Quinn B. Widely spread
butyrylcholinesterase can hydrolyze acetylcholine in the normal and
Alzheimer brain. Neurobiol Dis 2002;9:88-93.
6. Kashiwada Y, Aoshima A, Ikeshiro Y, Chen YP, Furukawa H,
Itoigawa M, et al. Anti-HIV benzylisoquinoline alkaloids and
flavonoids from the leaves of Nelumbo nucifera, and structure-activity
correlations with related alkaloids. Bioorg Med Chem 2005;13:443-8.
7. Bhardwaj A, Modi KP. A review on therapeutic potential of Nelumbo
nucifera (Gaertn): The sacred lotus. Int J Pharm Sci Res 2016;7:42-54.
8. He LS, Meng FL, Diao XJ, Li YW, Meng R, Xi BD, et al. Allelopathic
effect of Nelumbo nucifera stem and leaf tissue extract on the growth of
Microcystis aeruginosa and Scenedesmus quadricauda. Huan Jing Ke
Xue 2013;34:2637-41.
9. You JS, Lee YJ, Kim KS, Kim SH, Chang KJ. Anti-obesity and
hypolipidaemic effects of Nelumbo nucifera seed ethanol extract in
human pre-adipocytes and rats fed a high-fat diet. J Sci Food Agric
2014;94:568-75.
10. Rai S, Wahile A, Mukherjee K, Saha BP, Mukherjee PK. Antioxidant
activity of Nelumbo nucifera (sacred lotus) seeds. J Ethnopharmacol
2006;104:322-7.
11. Ahn YJ, Park SJ, Woo H, Lee HE, Kim HJ, Kwon G, et al. Effects of
allantoin on cognitive function and hippocampal neurogenesis. Food
Chem Toxicol 2014;64:210-6.
12. Ohkoshi E, Miyazaki H, Shindo K, Watanabe H, Yoshida A,
Yajima H, et al. Constituents from the leaves of Nelumbo nucifera
stimulate lipolysis in the white adipose tissue of mice. Planta Med
2007;73:1255?9.
13. Tho NT, An TN, Tri MD, Sreekanth TV, Lee JS, Nagajyothi PC, et al.
Green synthesis of silver nanoparticles using Nelumbo nucifera seed
extract and its antibacterial activity. Acta Chim Slov 2013;60:673-8.
14. Sohn DH, Kim YC, Oh SH, Park EJ, Li X, Lee BH, et al.
Hepatoprotective and free radical scavenging effects of Nelumbo
nucifera. Phytomedicine 2003;10:165-9.
15. Tsuruta Y, Nagao K, Kai S, Tsuge K, Yoshimura T, Koganemaru K,
et al. Polyphenolic extract of lotus root (edible rhizome of Nelumbo
nucifera) alleviates hepatic steatosis in obese diabetic db/db mice.
Lipids Health Dis 2011;10:202.
16. Zhou YJ, Xiang JZ, Yuan H, Liu H, Tang Q, Hao HZ, et al. Neferine
exerts its antithrombotic effect by inhibiting platelet aggregation
and promoting dissociation of platelet aggregates. Thromb Res
2013;132:202-10.
17. Zhou M, Jiang M, Ying X, Cui Q, Han Y, Hou Y, et al. Identification
and comparison of anti-inflammatory ingredients from different organs
of lotus Nelumbo by UPLC/Q-TOF and PCA coupled with a NF-?B
reporter gene assay. PLoS One 2013;8:e81971.
18. Yoon JS, Kim HM, Yadunandam AK, Kim NH, Jung HA, Choi JS,
et al. Neferine isolated from Nelumbo nucifera enhances anti-cancer
activities in hep3B cells: Molecular mechanisms of cell cycle arrest, ER
stress induced apoptosis and anti-angiogenic response. Phytomedicine
2013;20:1013-22.
19. Liu S, Li D, Huang B, Chen Y, Lu X, Wang Y, et al. Inhibition of
pancreatic lipase, ?-glucosidase, ?-amylase, and hypolipidemic effects
of the total flavonoids from Nelumbo nucifera leaves. J Ethnopharmacol
2013;149:263-9.
20. Niu CH, Wang Y, Liu JD, Wang JL, Xiao JH. Protective effects of
neferine on amiodarone-induced pulmonary fibrosis in mice. Eur J
Pharmacol 2013;714:112-9.
21. Mukherjee PK, Mukherjee D, Maji AK, Rai S, Heinrich M. The sacred
lotus (Nelumbo nucifera) phytochemical and therapeutic profile.
J Pharm Pharmacol 2009;61:407-22.
22. Arjun P, Priya SM, Sivan PS, Krishnamoorthy M, Balasubramanian K.
Antioxidant and antimicrobial activity of Nelumbo nucifera Gaertn.
leaf extracts. J Acad Ind Res 2012;1:15-8.
23. Yang D, Zhang Q, Ren G, Ying T. A comparative study on antioxidant
activity of different parts of lotus (Nelumbo nucifera Gaertn) rhizome.
Food Sci Technol (Campanas) 2017;37:135-8.
24. Liu CP, Tsai WJ, Lin YL, Liao JF, Chen CF, Kuo YC, et al. The extracts
from Nelumbo nucifera suppress cell cycle progression, cytokine
genes expression, and cell proliferation in human peripheral blood
mononuclear cells. Life Sci 2004;75:699-716.
25. Hu M, Skibsted LH. Antioxidative capacity of rhizome extract and
rhizome knot extract of edible lotus (Nelumbo nucifera). Food Chem
2002;76:327-33.
26. Wang QZ, Pen GH, Jin Y, Li J, Yan SL. Extraction of polyphenol
from lotus roots and its enzymatic browning substrate. J Anal Sci
2004;20:38?40.
27. Sridhar KR, Rajeev B. Lotus a potential nutraceutical source. J Agric
Technol 2007;3:143-55.
28. Ellman GL, Courtney KD, Andres V Jr., Feather-Stone RM. A new
and rapid colorimetric determination of acetylcholinesterase activity.
Biochem Pharmacol 1961;7:88-95.
29. Santillo MF, Liu Y. A fluorescence assay for measuring
acetylcholinesterase activity in rat blood and a human neuroblastoma
cell line (SH-SY5Y). J Pharmacol Toxicol Methods 2015;76:15-22.
30. Sun W, Chen L, Zheng W, Wei X, Wu W, Duysen EG, et al. Study of
acetylcholinesterase activity and apoptosis in SH-SY5Y cells and mice
exposed to ethanol. Toxicology 2017;384:33-9.
Published
15-09-2019
How to Cite
JARMKOM, K., TECHAOEI, S., WISIDSRI, N., & KHOBJAI, W. (2019). EVALUATION OF ACETYLCHOLINESTERASE ACTIVITY AND CYTOTOXICITY OF DIFFERENT PARTS OF NELUMBO NUCIFERA GAERTN ON HUMAN NEUROBLASTOMA CELL LINE (SH-SY5Y). International Journal of Applied Pharmaceutics, 11(5), 103–105. https://doi.org/10.22159/ijap.2019.v11s5.T0080
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