SYNTHESIS AND STABILITY OF RESVERATROL-CONJUGATED GOLD NANOPARTICLES MODIFIED WITH POLYETHYLENE GLYCOL
DOI:
https://doi.org/10.22159/ijap.2020.v12s1.FF050Keywords:
Gold nanoparticles, Polyethylene glycol, Resveratrol, StabilityAbstract
Objective: Gold nanoparticles (AuNPs) are highly useful for drug delivery, but their application is limited by their stability as they readily aggregate.
This issue can be prevented by adding a stabilizing agent such as resveratrol (RSV), which is a polyphenol derived from plants, that is used to prevent
cancer. Therefore, we propose a novel method to prepare stable RSV-conjugated nanoparticles modified with polyethylene glycol (RSV-AuNP-PEG).
Methods: In the first step, the Turkevich method was used to synthesize the AuNPs. Then, PEG was added as stabilizer agent and conjugated with RSV.
The synthesized conjugates were characterized using ultraviolet-visible spectrophotometry, Fourier transform infrared spectroscopy, particle size
analysis, and high-performance liquid chromatography.
Results: The obtained RSV-AuNP-PEG had a particle size of 83.93 nm with a polydispersity index (PDI) of 0.562 and formed a translucent purple-red
fluid in solution. The zeta potential was −22.9 mV, and the highest entrapment efficiency was 75.86±0.66%. For comparison, the RSV-AuNP solution
was purple and turbid, the particle size was 51.97 nm with a PDI of 0.694, and the zeta potential was −24.6 mV. The stability test results showed that
the storage stability of RSV-AuNP-PEG was better than that of AuNP-RSV. Further, the RSV-AuNP-PEG was shown to be most stable in 2% bovine serum
albumin (BSA) while the AuNP-RSV was most stable in 2% BSA in phosphate-buffered saline pH 7.4.
Conclusion: These results show that modification of RSV-conjugated AuNPs with PEG effectively prevents their aggregation in storage, but only in
certain mediums.
Downloads
References
Pharm Clin Res 2018;11:30-5.
2. Lavanya N, Muzib I, Jithan A, Umamahesh B. Novel nanoparticles for
the oral delivery of low molecular weight heparin: In vitro and in vivo
assessment. Asian J Pharm Clin Res 2017;10:254-61.
3. Rahme K, Chen L, Hobbs R, Morris M, O’Driscoll C, Holmes J.
PEGylated gold nanoparticles: Polymer quantification as a function of
PEG lengths and nanoparticle dimensions. RSC Adv 2013;3:6085-94.
4. Ajnai G, Chiu A, Kan T, Cheng C, Tsai T, Chang J. Trends of gold
nanoparticle-based drug delivery system in cancer therapy. J Exp Clin
Med 2014;6:172-8.
5. Rahme K, Nolan M, Doody T, McGlacken G, Morris M, O’Driscoll C,
et al. Highly stable PEGylated gold nanoparticles in water: Applications
in biology and catalysis. RSC Adv 2013;3:21016-24.
6. Djajadisastra J, Sutiyo S, Purnamasari P, Pujiyanto A. Antioxidant
activity of gold nanoparticles using gum Arabic as a stabilizing agent.
Int J Pharm Pharm Sci 2014;6:462-5.
7. Kalaivani A, Chellaram C. Nanoparticle PEGylation for imaging and
therapy- a review. Indian J Innov Dev 2012;1:52-3.
8. Kasiotis KM, Pratsinis H, Kletsas D, Haroutounian SA. Resveratrol and
related stilbenes: Their anti-aging and anti-angiogenic properties. Food
Chem Toxicol 2013;61:112-20.
9. Urbaniak A, Delgado M, Kacprzak K, Chambers TC. Activity of
resveratrol triesters against primary acute lymphoblastic leukemia cells.
Bioorg Med Chem Lett 2017;27:2766-70.
10. Park SY, Chae SY, Park JO, Lee KJ, Park G. Gold-conjugated
resveratrol nanoparticles attenuate the invasion and MMP-9 and COXInt
2 expression in breast cancer cells. Oncol Rep 2016;35:3248-56.
11. Kumar S, Gandhi KS, Kumar R. Modeling of formation of gold
nanoparticles by citrate method. Ind Eng Chem Res 2007;46:3128-36.
12. Dyah PR, Djajadisastra J, Mutalib A. Pujiyanto A. Preparation of gold
nanoparticles with based on conjugated gum Arabic vincristine and
evaluation of their in vitro characteristics. J Ilmu Kefarmasian Indones
2018;16:6-11.
13. Chen KS, Hung TS, Wu HM, Wu JY, Lin MT, Feng CK. Preparation
of thermosensitive gold nanoparticles by plasma pretreatment and UV
grafted polymerization. Thin Solid Films 2010;518:7557-62.
14. Turkevich J. Colloidal gold (part II): Colour, coagulation, adhesion,
alloying and catalytic properties. Gold Bull 1985;18:125-31.
15. Malvern Instrument. Dynamic Light Scattering: An Introduction in 30
Minutes. United Kingdom: Malvern Instrument; 2012.
16. Bhatia S. Natural Polymer Drug Delivery Systems: Nanoparticles
Types, Classification, Characterization, Fabrication Methods, and Drug
Delivery Applications. Switzerland: Springer; 2016.
17. Ramadon D, Wirarti G, Anwar E. Novel transdermal ethosomal
gel containing green tea (Camellia sinensis L. Kuntze) Leaves
extract: Formulation and in vitro penetration study. J Young Pharm
2017;9:336-40.
18. Hou Z, Zhan C, Jiang Q, Hu Q, Li L, Chang D, et al. Both FA- and
mPEG-conjugated chitosan nanoparticles for targeted cellular uptake
and enhanced tumor tissue distribution. Nanoscale Res Lett 2011;6:563.
19. Oliveira J, Prado A, Keijok W, Ribeiro M, Pontes M, Nogueira B,
et al. A helpful method for controlled synthesis of monodisperse
gold nanoparticles through response surface modeling. Arab J Chem
2017;13:1-11.
20. Butt H, Graf K, Kapp M. Physics and Chemistry of Interfaces.
Weinheim: Wiley-VCH; 2006.
21. Wu J, Zhao C, Lin W, Hu R, Wang Q, Chen H, et al. Binding
characteristics between polyethylene glycol (PEG) and proteins in
aqueous solution. J Mater Chem B 2014;2:2983.
22. Swietach P, Vaughan-Jones RD, Harris AL, Hulikova A. The chemistry,
physiology and pathology of pH in cancer. Philos Trans R Soc Lond B
Biol Sci 2014;369:20130099.
Published
How to Cite
Issue
Section
