ANTI-INFLAMMATORY EFFECT OF ETHANOL EXTRACT OF MAHKOTA DEWA [PHALERIA MACROCARPA (SCHEFF. BOERL)] LEAVES IN MOUSE COLITIS INDUCED BY AZOXYMETHANE (AOM) AND DEXTRAN SODIUM SULFATE (DSS): FOCUS ON RECTAL TISSUE

AFID BRILLIANA PUTRA; KUSMARDI KUSMARDI; ARYO TEDJO; ARI ESTUNINGTYAS; MUHAMMAD ILHAM DHIYA RAKASIWI

doi:10.22159/ijap.2020.v12s3.39491

Authors

AFID BRILLIANA PUTRA Bachelor Program for Medical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
KUSMARDI KUSMARDI Department of Pathologic Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
ARYO TEDJO Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
ARI ESTUNINGTYAS Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
MUHAMMAD ILHAM DHIYA RAKASIWI Bachelor Program for Medical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020.v12s3.39491

Keywords:

Ethanol extract, Goblet cells, Inflammation, Mahkota dewa (Phaleria macrocarpa), Rectum

Abstract

Objective: In this study, we aim to analyze the effect of this ethanol extract on the average number of goblet cells per crypt, number of inflammatory foci and number of angiogenesis in the rectal tissues of mice colitis-model that induced by azoxymethane (AOM) and dextran sodium sulfate (DSS).

Methods: This study was carried out by experimental in vivo using Balb/c mice. The mice were divided into five groups of treatment: normal, negative control (AOM/DSS), positive control (AOM/DSS+aspirin), EMD25 (AOM/DSS+25% ethanol extract) and EMD12.5 (AOM/DSS+12.5% ethanol extract). The mice were euthanized and their rectal tissues were placed on glass slides for histopathological observation using haematoxylin–eosin staining.

Results: The administration of the ethanol extract of mahkota dewa (Phaleria macrocarpa) leaves cannot inhibit the decrease in the average number of goblet cells per crypt (p=0.450) and cannot reduce the number of inflammation foci (p=0.146) and the number of angiogenesis (p=0.728). The ethanol extract of mahkota dewa (Phaleria macrocarpa) leaves could not inhibit the inflammation induced by AOM/DSS in the rectal tissues of mice. However, the extract has a tendency to maintain the average number of goblet cells per crypt.

Conclusion: Ethanol extract of Mahkota Dewa leaves can inhibit inflammation in mice's rectal tissue.

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References

Grivennikov SI. Inflammation and colorectal cancer: colitis-associated neoplasia. Semin Immunopathol 2013;35:229–44.

Strayer DS, Rubin E, Saffitz JE, Schiller AL. Rubin’s pathology: clinicopathologic foundations of medicine. In: 7th ed. Philadelphia: Wolters Kluwer Health/lippincott Williams and Wilkins; 2015. p. 55–7.

Ullman TA, Itzkowitz SH. Intestinal inflammation and cancer. Gastroenterology 2011;140:1807–16.

Feuerstein JD, Cheifetz AS. Ulcerative colitis: epidemiology, diagnosis, and management. Mayo Clin Proc 2014;89:1553–63.

Rubin D, Shaker A, Levin M. Chronic intestinal inflammation: inflammatory bowel disease and colitis-associated colon cancer. Front Immunol 2012;3:107.

Conaghan PJ, Mortensen NJMC. Inflammatory bowel disease: ulcerative colitis. In: Herold A, Lehur PA, Matzel KE, O’Connell PR. editors. Coloproctology. Berlin, Heidelberg: Springer Berlin Heidelberg; 2017. p. 157–76.

Kelompok Studi Inﬂammatory Bowel Disease Indonesia. Konsensus nasional penatalaksanaan inﬂammatory bowel disease (IBD) di Indonesia. Jakarta: Perkumpulan Gastroenterologi Indonesia; 2011.

Sands BE. New therapies for the treatment of inflammatory bowel disease. Surg Clin North Am 2006;86:1045–64.

Romano M, De Francesco F, Zarantonello L, Ruffolo C, Ferraro GA, Zanus G, et al. From inflammation to cancer in inflammatory bowel disease: molecular perspectives. Anticancer Res 2016;36:1447–60.

Hendra R, Ahmad S, Oskoueian E, Sukari A, Shukor MY. Antioxidant, anti-inflammatory and cytotoxicity of Phaleria macrocarpa (Boerl.) Scheff fruit. BMC Complement Altern Med 2011;11:110.

Suprapti T, Louisa M, Tedjo A, Kusmardi K, Fadilah F. Antiinflammatory effect of mahkota dewa (Phaleria macrocarpa (Scheff.) (Boerl.) leaves extract on colon carcinogenesis induced by azoxymethane and dextran sodium sulphate: focus on the iNOS, β-catenin and COX-2 expression. Asian Aust J Anim Sci 2014;2:511–27.

Kusmardi K, Estuningtyas A, Shavera D, Tedjo A, Priosoeryanto B. The effect of mahkota dewa (Phaleria macrocarpa) (scheff.) fruit pericarp extract on inos in mice colon intermittently-induced by dextran sodium sulfate. Asian J Pharm Clin Res 2017;10:309–12.

Dahlan MS. Statistik untuk Kedokteran dan Kesehatan: Deskriptif, Bivariat, dan Multivariat. In: 6th ed. Jakarta: Epidemiologi Indonesia; 2017. p. 110.

Estuningtyas A, Widiasari S, Louisa M, Kusmardi K. The effect of mahkota dewa (Phaleria macrocarpa l.) leaf extract encapsulated in chitosan nanoparticles on iNOS and COX-2 expression in dextran sodium sulfate-induced colitis mice model. J Int Dent Med Res 2019;12:220–7.

Kusmardi K, Tamzir AR, Widiasari S, Estuningtyas A. Suppression effect of mahkota dewa (Phaleria macrocarpa) leaf extract in chitosan nanoparticles on the small intestine of dextran sulfate sodium-induced mice: focus on mitosis and hyperplasia. Asian J Pharm Clin Res 2018;11:118-20.

Erben U, Loddenkemper C, Doerfel K, Spieckermann S, Haller D, Heimesaat MM, et al. A guide to the histomorphological evaluation of intestinal inflammation in mouse models. Int J Clin Exp Pathol 2014;7:4557–76.

Estuningtyas A, Widiasari S, Kusmardi K. Acute toxicity of chitosan nanoparticles containing mahkota dewa (Phaleria macrocarpa) leaf extract and anti-inflammatory effects in a dextran sodium sulfate-induced mouse model of ulcerative colitis. Int J Appl Pharm 2018;10:6-10.

Chan SSM, Luben R, Bergmann MM, Boeing H, Olsen A, Tjonneland A, et al. Aspirin in the aetiology of crohn’s disease and ulcerative colitis: a European prospective cohort study. Aliment Pharmacol Ther 2011;34:649–55.

Ananthakrishnan AN, Higuchi LM, Huang ES, Khalili H, Richter JM, Fuchs CS, et al. Aspirin, nonsteroidal anti-inflammatory drug use, and risk for Crohn disease and ulcerative colitis: a cohort study. Ann Intern Med 2012;156:350–9.

Alam MN, Almoyad M, Huq F. Polyphenols in colorectal cancer: current state of knowledge including clinical trials and molecular mechanism of action. Biomed Res Int 2017;2018:1–30.

Din FVN, Theodoratou E, Farrington SM, Tenesa A, Barnetson RA, Cetnarskyj R, et al. Effect of aspirin and NSAIDs on risk and survival from colorectal cancer. Gut 2010;59:1670–9.