POTENTIAL OF ETHANOL EXTRACT OF MAHKOTA DEWA LEAVES (PHALERIA MACROCARPA (SCHECFF.) BOERL.) TO INHIBIT INFLAMMATION IN MOUSE DISTAL COLON INDUCED BY DEXTRAN SODIUM SULFATE (DSS) AND AZOXYMETHANE (AOM)

MUHAMMAD ILHAM DHIYA RAKASIWI; KUSMARDI KUSMARDI; ARI ESTUNINGTYAS; ARYO TEDJO

doi:10.22159/ijap.2020.v12s3.39490

Authors

MUHAMMAD ILHAM DHIYA RAKASIWI Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
KUSMARDI KUSMARDI Department of Pathological Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
ARI ESTUNINGTYAS Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
ARYO TEDJO Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020.v12s3.39490

Keywords:

Angiogenesis, Distal colon, Ethanol extract, Goblet cell, Phaleria macrocarpa leaf

Abstract

Objective: To demonstrates the ability of P. macrocarpa leaf extract to reduce inflammation of the distal colon in DSS/AOM-induced mice.

Methods: In vivo experimental research using Balb/c mice induced by 0.2 ml azoxymethane (AOM) 0.1% once and 1% dextran sodium sulphate (DSS) for one week; additionally, ethanol extract of P. macrocarpa leaves, 25 mg and 50 mg, and 0.84 mg acetosal were given orally. The mice were sacrificed after 20 w. Histopathological examination (hematoxylin-eosin staining) was conducted by counting the average number of goblet cells per crypt, inflammatory focus and angiogenesis.

Results: Ethanol extract of P. macrocarpa leaves was able to prevent the decrease in the number of goblet cells (p<0.05). However, the administration of ethanol P. macrocarpa leaf extract could not reduce focal inflammation and angiogenesis in inflammation of the distal colon.

Conclusion: Ethanol extract of the Mahkota Dewa leaves is able to prevent inflammation of the distal colon by preventing the decrease in the number of goblet cells.

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