THE DETERMINATION OF ETHYL P-METHOXY CINNAMATE IN KAEMPFERIA GALANGA L. RHIZOME EXTRACT HARVESTED IN RAINY AND DRY SEASONS

Authors

  • INDAH SUASANI WAHYUNI Doctoral Program in Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Sumedang, West Java, Indonesia, Department of Oral Medicine, Faculty of Dentistry, Universitas Padjadjaran, Bandung, West Java, Indonesia
  • IRNA SUFIAWATI Department of Oral Medicine, Faculty of Dentistry, Universitas Padjadjaran, Bandung, West Java, Indonesia
  • WIPAWEE NITTAYANANTA Faculty of Dentistry, Thammasat University, Pathum Thani, Thailand
  • JUTTI LEVITA Doctoral Program in Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Sumedang, West Java, Indonesia, Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Sumedang, West Java, Indonesia.

DOI:

https://doi.org/10.22159/ijap.2021.v13s4.43841

Keywords:

Anti-inflammation, Ethyl p-methoxycinnamate, Herbal medicine, Kaempferia galanga L

Abstract

Objective: Kaempferia galanga L. rhizome (KGR), has been empirically used in Indonesia, particularly by Javanese, to cure inflammation. KGR contains various secondary metabolites which explain its pharmacology activities, among them is ethyl p-methoxycinnamate (EPMC). However, due to the different seasons of our country, the yield of extraction is often unalike. In this work, we determined the percentage of yield (w/w), the water content (thermogravimetric method), and the concentration of EPMC in the Ethanol extract of Kaempferia galanga L. Rhizome (EEKG) harvested from the rainy (EEKG-R) and dry seasons (EEKG-D).

Methods: The sun-dried rhizomes were cold macerated for 3x24 h with 70% ethanol, filtered, and the solvent was evaporated at 40-45 °C until a viscous extract was obtained. The determination of EPMC in the extract was carried out using the RP-HPLC standard addition method. Detection was set at 308 nm; injection volume 20 µl; flow rate 1.0 ml/min. The column used is C18 (length 250 mm, internal diameter 4.6 mm, particle size 5 µm).

Results: The yield of EEKG-R (harvested in the rainy season) = 14.56% w/w, water content = 4.37%, and the EPMC = 0.01%. Meanwhile the yield of EEKG-D (harvested in the dry season) = 5.79% w/w, water content = 18.76%, and the EPMC = 0.001%.

Conclusion: Different climates affect the percentage yield and the quality of the extract. In our work, the EEKG-R (harvested in the rainy season) revealed a better quality compared to that of EEKG-D (harvested in the dry season) This study gives important information to standardize and optimize the harvest time of KG rhizomes for drugs development, which are strongly influenced by seasonal differences.

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References

Aroonrerk N, Kamkaen N. Anti-inflammatory Activity of Quercus infectoria, Glycyrrhiza uralensis, Kaempferia galanga and Coptis chinensis, the Main Components of Thai Herbal Remedies for Aphthous Ulcer. J Health Res. 2009;23(1):17-22.

Umar I. Phytochemistry and medicinal properties of Kaempferia galanga L. (Zingiberaceae) extracts. (Zingiberaceae) extracts. Afr J Pharm Pharmacol. 2011;5(14):1638-47. doi: 10.5897/AJPP11.388.

Kusumawati I, Hestianah EP, Yusuf H. Republic; 2018. Available from: https://www.researchgateResearchgate.net/publication/330409630_Topical_Analgesic_from_Kaempferia_galanga_extract Indonesia Patent IDP000053030. [Last accessed on 07 Oct 2021]

Levita J, K. Wijaya LK, Celcilia S, Mutakin M. Inhibitory activity of kaempferia galanga and hibiscus sabdariffa on the Rate of PGH2 formation. J Appl Sci. 2015;15(7):1032-6. doi: 10.3923/jas.2015.1032.1036.

Ridtitid W, Sae-wong C, Reanmongkol W, Wongnawa M. Antinociceptive activity of the methanolic extract of Kaempferia galanga Linn. in experimental animals. J Ethnopharmacol. 2008;118(2):225-30. doi: 10.1016/j.jep.2008.04.002, PMID 18486374.

Samodra G, Febrina D. Anti-inflammatory Effects of Kaempferia galanga L. rhizome extract in carrageenan-induced female rats. Adv Health Sci Res. 2019;20(1):13-7.

Riasari H, Rachmaniar R, Febriani Y. Effectiveness of anti-inflammatory plaster from Kencur (Kaempferia Galanga L.) rhizome ethanol extract. Int J Pharm Sci Res. 2016;7(4):1746-9.

Jagadish PC, Latha KP, Mudgal J, Nampurath GK. Extraction, characterization and evaluation of Kaempferia galanga L. (Zingiberaceae) rhizome extracts against acute and chronic inflammation in rats. J Ethnopharmacol. 2016;194:434-9. doi: 10.1016/j.jep.2016.10.010, PMID 27720847.

Umar MI, Asmawi MZ, Sadikun A, Atangwho IJ, Yam MF, Altaf R, Ahmed A. Bioactivity-Guided Isolation of ethyl-p-methoxycinnamate, an anti-inflammatory Constituent, from Kaempferia galanga L. extracts. Molecules. 2012;17(7):8720-34. doi: 10.3390/molecules17078720, PMID 22825623.

Umar MI, Asmawi MZ, Sadikun A, Majid AMSA, Al-Suede FSR, Hassan LEA, Altaf R, Ahamed MB. Ethyl-p-methoxycinnamate isolated from Kaempferia galanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-α, and angiogenesis by blocking endothelial functions. Clinics (Sao Paulo). 2014;69(2):134-44. doi: 10.6061/clinics/2014(02)10, PMID 24519205.

Yang L, Wen KS, Ruan X, Zhao YX, Wei F, Wang Q. Response of plant secondary metabolites to environmental factors. Molecules. 2018;23(4):762. doi: 10.3390/molecules23040762, PMID 29584636.

Isah T. Stress and defense responses in plant secondary metabolites production. Biol Res. 2019;52(1):39. doi: 10.1186/s40659-019-0246-3, PMID 31358053.

Mukkasombut N, Pipatrattanaseree W, Itharat A. Validation of HPLC method for the determination of anti-allergic compounds in ethanolic extract of prasaprohyai remedy, a thai traditional medicine. Thammasat Med J. 2020;20(1):74-83.

Kaushita B, Priya CL, Rao KVB. HPLC analysis and antioxidant activities of hydroethanolic leaf extract of Kaempferia galanga Linn. Int J PharmTech Res. 2014;7(2):422-31.

Raj R, Chandrashekar KS, Biswas R, Pai A, Pai V. Rapid high-performance thin layer chromatographic quantitative estimation of caffeine in various foods and beverages. Rasayan J Chem. 2021;14(1):221-6. doi: 10.31788/RJC.2021.1415991.

daerah Kabupaten P. Sumedang. Iklim [internet]. Available from: https://www.subang.go.ID/profil/iklim. [Last accessed on 10 Jul 2021]

Wahyuni IS, Sufiawati I, Nittayananta W, Levita J. Identification of ethyl para-methoxycinnamate and kaempferol in the ethanol extract of Kaempferia galanga L. Rhizome as biomaterial for drug candidate using spectrophotometric and chromatographic analysis. Mater Sci Forum. 2021;1028:371-6. doi: 10.4028/www.scientific.net/MSF.1028.371.

Saadatkhah N, Carillo Garcia AC, Ackermann S, Leclerc P, Latifi M, Samih S, Patience GS, Chaouki J. Experimental methods in chemical engineering: thermogravimetric analysis-TGA. Can J Chem Eng. 2020;98(1):34-43. doi: 10.1002/cjce.23673.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Int Pharm Sci. 2011;1(1):98-106.

Kementerian kesehatan republik Indonesia. Direktorat Jendral Kefarmasian dan Alat Kesehatan. Farmakope Herbal Indonesia; 2017.

Nurani LH, Asahi A, Susanti H. Determination of epms content and anti-inflammatory test rhizome extract Kaempferia galanga, L by inhibition of protein denaturation method. Pharmaciana. 2020;10(3):381-90. doi: 10.12928/pharmaciana.v10i3.15293.

Riasari H, Rachmaniar R, Wahyuni S. Evaluation patch of rhizoma extract kencur (Kaempferia galanga L.) as anti-inflammatory with enhancer. Indonesian J Pharm Sci Technol. 2019;6(2):59-64. doi: 10.24198/ijpst.v6i2.18932.

Cekur KH. (Kaempferia galanga L.) in: Penanaman Tumbuhan Ubatan and Beraroma [Internet]. Com/index. php? option=com_contentandview=articleandid=106089:kaempferia-galanga-landcatid=286andItemid=357#r6. Available from: https://globinmed. [Last accessed on 07 Oct 2021]

Lee JE, Oliveira RS, Dawson TE, Fung I. Root functioning modifies seasonal climate. Proc Natl Acad Sci USA. 2005;102(49):17576-81. doi: 10.1073/pnas.0508785102. PMID 16301519.

Dawson TE, Pate JS. Seasonal water uptake and movement in root systems of Australian phraeatophytic plants of dimorphic root morphology: a stable isotope investigation. Oecologia. 1996;107(1):13-20. doi: 10.1007/BF00582230, PMID 28307187.

Sirisangtragul W, Jarukamjorn K, Nemoto N, Yenjai C, Sripanidkulchai B. Effect of ethyl-p-methoxy cinnamate from Kaempferia galanga on cytochrome P450 enzymes expression in mouse hepatocytes. Chiang Mai J Sci. 2011;38(3):453-62.

Amuamuta A, Plengsuriyakarn T, Na-Bangchang K. Anticholangiocarcinoma activity and toxicity of the Kaempferia galanga Linn. Rhizome ethanolic extract. BMC Complement Altern Med. 2017;17(1):213. doi: 10.1186/s12906-017-1713-4, PMID 28403856.

Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga Latha LY. Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr J Tradit Complement Altern Med. 2011;8(1):1-10. doi: 10.4314/ajtcam.v8i1.60483, PMID 22238476.

Srivastava N, Ranjana, Singh S, Gupta AC, Shanker K, Bawankule DU, Luqman S. Aromatic ginger (Kaempferia galanga L.) extracts with ameliorative and protective potential as a functional food, beyond its flavor and nutritional benefits. Toxicol Rep. 2019;6:521-8. doi: 10.1016/j.toxrep.2019.05.014, PMID 31431884.

Published

11-12-2021

How to Cite

WAHYUNI, I. S., SUFIAWATI, I., NITTAYANANTA, W., & LEVITA, J. (2021). THE DETERMINATION OF ETHYL P-METHOXY CINNAMATE IN KAEMPFERIA GALANGA L. RHIZOME EXTRACT HARVESTED IN RAINY AND DRY SEASONS. International Journal of Applied Pharmaceutics, 13(4), 132–135. https://doi.org/10.22159/ijap.2021.v13s4.43841

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