• YESI DESMIATY Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia
  • FAIZATUN FAIZATUN Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia
  • YUSLIA NOVIANI Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia
  • HESTIARY RATIH Faculty of Pharmacy, Universitas Jenderal Achmad Yani, Cimahi, West Java, Indonesia
  • NENENG SITI SILFI AMBARWATI Faculty of Engineering, Universitas Negeri Jakarta, Jakarta, Indonesia



Premature skin aging, In vitro test, Plant extract, Elastase enzyme, Collagenase enzyme


The objective of this article is to review the phytochemicals that serve as anti-aging materials by developing in vitro activity screening test methods. The method used is a literature study from national and international journals, including the original article, research article, etc., with a total of over 40 journals. Secondary metabolite compounds from natural ingredients such as flavonoids, polyphenols, alkaloids, and terpenes have antioxidant activity and have the potential to be anti-aging substances for the skin. The initial stage of searching for natural ingredients that have anti-aging activity can be done by developing in vitro activity screening test methods, such as inhibition of collagenase, elastase, hyaluronidase, and tyrosinase enzymes. This enzyme plays an important role as one of the causes of premature aging of the skin. From the literature search, it was found that many plant extracts to active compounds and cosmetic preparations from the extracts can inhibit these enzymes.  The group of compounds that are known to have acted as enzyme inhibitors is polyphenolic compounds, especially those with multimer properties, flavonoids, especially those with ortho-OH groups, triterpenes (especially pentacyclic), essential oils, and others. This shows that natural ingredients are one source of potential compounds as raw materials in the formulation of anti-aging cosmetic preparations.


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