ISOLATION, IDENTIFICATION, AND ANTIBACTERIAL OF AMENTOFLAVONE FROM GARCINIA LATISSIMA MIQ. LEAVES

NENENG SITI SILFI AMBARWATI; BERNA ELYA; AMARILA MALIK; MUHAMMAD HANAFI; MUHAMAD SHARUL NIZAM AWANG

doi:10.22159/ijap.2022.v14s3.13

Authors

NENENG SITI SILFI AMBARWATI Faculty of Engineering, Universitas Negeri Jakarta, Jl. Rawamangun Muka, East Jakarta 13220, Indonesia
BERNA ELYA Faculty of Pharmacy, Universitas Indonesia, UI Depok Campus, Depok 16424, West Java, Indonesia
AMARILA MALIK Faculty of Pharmacy, Universitas Indonesia, UI Depok Campus, Depok 16424, West Java, Indonesia
MUHAMMAD HANAFI Faculty of Pharmacy, Universitas Pancasila, Jl. Srengseng Sawah, Jagakarsa, South Jakarta 12640, Indonesia
MUHAMAD SHARUL NIZAM AWANG Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia

DOI:

https://doi.org/10.22159/ijap.2022.v14s3.13

Keywords:

Garcinia latissima Miq., Bacillus subtilis, amentoflavone

Abstract

Objective: This study aimed to isolate and identify the Bacillus subtilis antibacterial compound present in the leaves of the Garcinia latissima Miq.

Methods: The extracts were obtained by maceration successively, and the active extract was fractionated by column chromatography. The isolation of the most active fraction was performed by open column chromatography and preparative thin-layer chromatography. The isolate antibacterial assay against Bacillus subtilis by microdilution method. The compound structure of the isolate was identified by the nuclear magnetic resonance method.

Results: The most active extract of the extracts from Garcinia latissima Miq. leaves against Bacillus subtilis are the methanol extract. The most active fractions of the fractions from G. latissima Miq. Leaves methanolic extract was isolated. The active isolate was identified as Amentoflavone.

Conclusion: G. latissima Miq. leaves had the potential to be used as antibacterial medicinal herbs and one of the isolates of G. latissima Miq. is amentoflavone (active against B. Subtilis).

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