STANDARDIZATION AND A-GLYCOSIDASE INHIBITION OF EXTRACTS AND NANOPARTICLE EXTRACT OF KEMBANG BULAN LEAVES (TITHONIA DIVERSIVOLIA (HAMSLEY) A. GRAY)
Keywords:Kembang Bulan leaves, (Tithonia diversifolia (Hamsley) A. Gray), Nanoparticles
Objective: The purpose of this study was the determination of the quality parameters of the antidiabetic activity of 70% ethanol extract and Kembang Bulan leaf nanoparticle extract.
Methods: Kembang Bulan powder was macerated kinetic with 70% ethanol, evaporated until a thick extract was obtained using a rotary evaporator, then specific and non-specific parameters were determined, and phytochemical screening was done. The extract was tested for anti-diabetic activity in vitro using the α-glucosidase enzyme.
Results: Phytochemical screening results show the presence of flavonoids, saponins, tannins, essential oils, coumarin, steroids, and triterpenoids. The results of quality parameter examination showed that the extract had a thick consistency, blackish-brown color, aromatic odor, 75.20% dissolved compound and 71.97% dissolved compound in ethanol, 9.65% drying shrinkage, 8.73% moisture content, the remaining solvent is 0.43%, the total ash content is 7.12%, the acid insoluble ash content is 0.93%, the Pb level is 0.1167 mg/kg BW, the Cd level is 0.0620 mg/kg BW, the ALT microbial contamination is 0.6827x and Mold and yeast contamination 0.156x. Total flavonoid levels were 1.15%.
Conclusion: The test results can be concluded that the 70% ethanol extract and nanoparticle extracts from Kembang Bulan leaves can inhibit the activity of the αglucosidase enzyme and the inhibitory results obtained by the nanoparticle extract are greater than the 70% ethanol extract.
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