TOPOISOMERASE INHIBITORS ACTIVITY OF YELLOW CHAMPAKA (MICHELIA CHAMPACA L.) BARK EXTRACTS AND FRACTIONS AND ITS LIRIODENINE CONTENTS

ADE ZUHROTUN; YUNI ELSA HADISAPUTRI; FREDERICK ALEXANDER; RUDY SUSENO; IMAM ADI WICAKSONO

doi:10.22159/ijap.2022.v14s4.PP37

Authors

ADE ZUHROTUN Biological Pharmacy Department, Faculty of Pharmacy, Universitas Padjadjaran, Jalan Ir. Soekarno Km. 21 Jatinangor, Sumedang West Java, Indonesia 45363
YUNI ELSA HADISAPUTRI Biological Pharmacy Department, Faculty of Pharmacy, Universitas Padjadjaran, Jalan Ir. Soekarno Km. 21 Jatinangor, Sumedang West Java, Indonesia 45363
FREDERICK ALEXANDER Biological Pharmacy Department, Faculty of Pharmacy, Universitas Padjadjaran, Jalan Ir. Soekarno Km. 21 Jatinangor, Sumedang West Java, Indonesia 45363
RUDY SUSENO Biological Pharmacy Department, Faculty of Pharmacy, Universitas Padjadjaran, Jalan Ir. Soekarno Km. 21 Jatinangor, Sumedang West Java, Indonesia 45363
IMAM ADI WICAKSONO Pharmacology and Clinical Pharmacy Department, Faculty of Pharmacy, Universitas Padjadjaran, Jalan Ir. Soekarno Km. 21 Jatinangor, Sumedang West Java, Indonesia 45363

DOI:

https://doi.org/10.22159/ijap.2022.v14s4.PP37

Keywords:

Yellow champaka, Michelia champaca L., Topoisomerase inhibitors, Mechanism-based yeast bioassay

Abstract

Objective: This research is aimed to investigate the activity of various extracts and fractions of yellow champaka (Michelia champaca L.) by mechanism-based yeast bioassay (MBYB) against the mutant yeasts. Liriodenin content, an active compound in M. champaca, will analyze to know the correlation with this assay.

Methods: The bark of M. champaca was extracted by maceration and graded maceration using n-hexane, ethyl acetate, and methanol. The methanol extract was fractionated using water, n-hexane and ethyl acetate in the liquid-liquid extraction process. Each extract and fractions were tested in vitro by mechanism-based yeast bioassay for their topoisomerase inhibitor activity. Liriodenine content of all samples was analyzed by thin layer chromatography (TLC) densitometry method.

Results: The yeast bioassay results showed that all extracts were active as topoisomerase inhibitors (IC₁₂ values under 8000 µg/ml) except MGM. Liriodenine can be used as a marker of active compound in ethyl acetate samples that were EAM, EAGM and EAF with Pearson analysis value-0.887 (P=0.153) and these samples were relatively more active than others.

Conclusion: This research showed that various extracts and fractions of yellow champaka bark have topoisomerase inhibitor's activity. Liriodenine can be used as a marker of the active compound so yellow champak bark is a potent natural agents for breast cancer.

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