PHYSICAL AND CHEMICAL EVALUATIONS OF TOPICAL RADIANCE SERUM CONTAINING NANOEMULSION COMBINATION OF ASTAXANTHIN AND ZEAXANTHIN: DESIGNED AS ANTI-WRINKLE AND SKIN-BRIGHTENING SERUM

Authors

  • LUSI NURDIANTI Faculty of Pharmacy, Bakti Tunas Husada University, Tasikmalaya, Indonesia https://orcid.org/0000-0002-3623-6037
  • FAJAR SETIAWAN Faculty of Pharmacy, Bakti Tunas Husada University, Tasikmalaya, Indonesia https://orcid.org/0009-0004-2435-0483
  • TAOFIK RUSDIANA Faculty of Pharmacy, Padjadjaran University, Sumedang, Indonesia
  • DOLIH GOZALI Faculty of Pharmacy, Padjadjaran University, Sumedang, Indonesia
  • KENI IDA CAHYATI Faculty of Pharmacy, Bakti Tunas Husada University, Tasikmalaya, Indonesia https://orcid.org/0000-0002-4048-4340

DOI:

https://doi.org/10.22159/ijap.2023v15i5.48374

Keywords:

Astaxanthin, Zeaxanthin, Nanoemulsion, Radiance serum

Abstract

Objective: The present study was conducted to formulate and evaluate the radiance serum containing the combination of astaxanthin and zeaxanthin nanoemulsion designed for anti-wrinkle and skin brightening serum by topical route of administration.

Methods: The nanoemulsion containing astaxanthin and zeaxanthin was prepared using the self-nano emulsifying method, followed by incorporation into the radiance serum by the conventional mixing method. The quantity and ratio of surfactant, co-surfactant, and oil phase have been optimized in the previous study, as well as the radiance serum formula. The evaluation of the nanoemulsion and radiance serum was carried out by physical and chemical characterization. At the end of the study, an antioxidant activity of the serum containing nanoemulsion of astaxanthin and zeaxanthin was performed by DPPH method and the antioxidant activity was compared to its pure forms. The evaluation of the ex vivo permeation study was carried out to evaluate its possibility as an anti-wrinkle and skin brightener.

Results: An astaxanthin and zeaxanthin nanoemulsion had a good physical properties with a globule size around of 20 nm (narrow particle size distribution), an entrapment efficiency range greater than 85%, and had a spherical morphology. The radiance serum had a good organoleptic and spreadability with the semifluid characteristic. Based on the result of antioxidant activity, the radiance serum had a highly active antioxidant activity. The radiance serum contained of astaxanthin and zeaxanthin nanoemulsion of 1% concentration, astaxanthin had a 2-6 times cumulative released compared than zeaxanthin (p<0.05) and all of the formulations exhibited a high skin permeation significantly.

Conclusion: A formulation of nanoemulsion-based serum containing astaxanthin and zeaxanthin for topical delivery has been successfully developed. Based on the results of physical evaluation and especially from the permeation study, it seems that radiance serum containing astaxanthin and zeaxanthin nanoemulsion was potential to be used as an anti-wrinkle and skin brightening, however this function must be proven in further research.

Downloads

Download data is not yet available.

References

Clark RM, Yao L, She L, Furr HC. A comparison of lycopene and astaxanthin absorption from corn oil and olive oil emulsions. Lipids. 2000 Jul;35(7):803-6. doi: 10.1007/s11745-000-0589-8, PMID 10941883.

Sparrow JR, Kim SR. The carotenoids of macular pigment and bisretinoid lipofuscin precursors in photoreceptor outer segments. Carotenoids: physical, chemical and biological functions and properties. FL: CRC Press; 2009. p. 355-63.

Madhavi D, Kagan D, Seshadri S. A study on the bioavailability of a proprietary, sustained-release formulation of astaxanthin. Integr Med Clin’s J. 2018 Jun;17(3):38.

Parker RS. Absorption, metabolism, and transport of carotenoids. FASEB J. 1996 Apr;10(5):542-51. doi: 10.1096/fasebj.10.5.8621054, PMID 8621054.

Zaripheh S, Erdman Jr JW. Factors that influence the bioavailablity of xanthophylls. J Nutr. 2002 Mar 1;132(3):531S-4S. doi: 10.1093/jn/132.3.531S, PMID 11880587.

Chitchumroonchokchai C, Failla ML. Hydrolysis of zeaxanthin esters by carboxyl ester lipase during digestion facilitates micellarization and uptake of the xanthophyll by caco-2 human intestinal cells. J Nutr. 2006 Mar 1;136(3):588-94. doi: 10.1093/jn/136.3.588, PMID 16484529.

Niesor EJ, Chaput E, Mary JL, Staempfli A, Topp A, Stauffer A. Effect of compounds affecting ABCA1 expression and CETP activity on the HDL pathway involved in intestinal absorption of lutein and zeaxanthin. Lipids. 2014 Dec;49(12):1233-43. doi: 10.1007/s11745-014-3958-8, PMID 25300953.

Choi HD, Kang HE, Yang SH, Lee MG, Shin WG. Pharmacokinetics and first-pass metabolism of astaxanthin in rats. Br J Nutr. 2011 Jan;105(2):220-7. doi: 10.1017/S0007114510003454, PMID 20819240.

Zouboulis CC, Makrantonaki E. Clinical aspects and molecular diagnostics of skin aging. Clin Dermatol. 2011 Jan 1;29(1):3-14. doi: 10.1016/j.clindermatol.2010.07.001, PMID 21146726.

Kammeyer A, Luiten RM. Oxidation events and skin aging. Ageing Res Rev. 2015 May 1;21:16-29. doi: 10.1016/j.arr.2015.01.001, PMID 25653189.

Davinelli S, Bertoglio JC, Polimeni A, Scapagnini G. Cytoprotective polyphenols against chronological skin aging and cutaneous photodamage. Curr Pharm Des. 2018 Jan 1;24(2):99-105. doi: 10.2174/1381612823666171109102426, PMID 29119916.

Leyden JJ. Clinical features of ageing skin. Br J Dermatol. 1990 Apr;122 Suppl 35:1-3. doi: 10.1111/j.1365-2133.1990.tb16118.x, PMID 2186777.

Nurdianti L, Aryani R, Indra I. Formulasi dan karakterisasi SNE (Self Nanoemulsion) astaxanthin dari haematococcus pluvialis sebagai super antioksidan alami. J Sains Farm Klinis. 2017 Dec 15;4(1):36-42.

Nurdianti L, Clara R, Suhendy H, Setiawan F, Idacahyati K. Formulation, characterization, and determination of the diffusion rate study of antioxidant serum containing astaxanthin nanoemulsion. Int J App Pharm. 2021 Oct 01;13(4):200-4. doi: 10.22159/ijap.2021.v13s4.43859.

Nurdianti L, Setiawan F, Rusdiana T, Gozali D, Idacahyati K, Wulandari WT. Formulation and characterization of zeaxanthin nanoemulsion radiance serum as antioxidant. Int J Appl Pharm. 2022 Aug 20;14(4):116-20.

Date AA, Desai N, Dixit R, Nagarsenker M. Self-nanoemulsifying drug delivery systems: formulation insights, applications and advances. Nanomedicine (Lond). 2010 Dec;5(10):1595-616. doi: 10.2217/nnm.10.126, PMID 21143036.

Abbas IK. Self-nano emulsifying drug delivery system: liquid, Supersaturable, and solid dosage forms. Al-Rafidain J Med Sci. 2022 Dec 11;3:98-108. doi: 10.54133/ajms.v3i.91.

Gao L, Zhang D, Chen M. Drug nanocrystals for the formulation of poorly soluble drugs and its application as a potential drug delivery system. J Nanopart Res. 2008 May;10(5):845-62. doi: 10.1007/s11051-008-9357-4.

Clogston JD, Patri AK. Zeta potential measurement. Characterization of nanoparticles intended for drug delivery. NJ: Humana Press; 2011. p. 63-70.

Garg A, Aggarwal D, Garg S, Singla AK. Spreading of semisolid formulations: an update. Pharm Technol N Am. 2002;26(9):84.

Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem. 2005 Mar 23;53(6):1841-56. doi: 10.1021/jf030723c, PMID 15769103.

Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol. 2004 Dec;26(2):211-9.

Reynertson KA. Phytochemical analysis of bioactive constituents from edible myrtaceae fruits [dissertation]. New York: City University of New York; 2007.

Davinelli S, Nielsen ME, Scapagnini G. Astaxanthin in skin health, repair, and disease: a comprehensive review. Nutrients. 2018 Apr 22;10(4):522. doi: 10.3390/nu10040522, PMID 29690549.

Prausnitz MR, Langer R. Transdermal drug delivery. Nat Biotechnol. 2008 Nov;26(11):1261-8. doi: 10.1038/nbt.1504, PMID 18997767.

Published

07-09-2023

How to Cite

NURDIANTI, L., SETIAWAN, F., RUSDIANA, T., GOZALI, D., & CAHYATI, K. I. (2023). PHYSICAL AND CHEMICAL EVALUATIONS OF TOPICAL RADIANCE SERUM CONTAINING NANOEMULSION COMBINATION OF ASTAXANTHIN AND ZEAXANTHIN: DESIGNED AS ANTI-WRINKLE AND SKIN-BRIGHTENING SERUM. International Journal of Applied Pharmaceutics, 15(5), 221–226. https://doi.org/10.22159/ijap.2023v15i5.48374

Issue

Section

Original Article(s)

Most read articles by the same author(s)

<< < 1 2 3 > >>