OPTIMIZATION OF LACTOBACILLUS PLANTARUM FERMENTATION FOR ENHANCED PHENOLIC PRODUCTION FROM EXTRACT OF BAJAKAH BARK (UNCARIA NERVOSA) BY RESPONSE SURFACE METHODOLOGY

Authors

  • MARIA ALMEIDA Doctor of Pharmacy Program, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor-45363, West Java, Indonesia
  • ANGGA CIPTA NARSA Department of Pharmaceuticals and Pharmaceutical Technology, Faculty of Pharmacy, Mulawarman University, Samarinda, Indonesia
  • SRI AGUNG FITRI KUSUMA Department of Biology Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor-45363, West Java, Indonesia
  • SRIWIDODO SRIWIDODO Department of Pharmaceuticals and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor-45363, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2023.v15s2.23

Keywords:

Bajakah, Propionibacterium acnes, Lactobacillus plantarum, Phenolic, Response surface methodology

Abstract

Objective: This research was purposed to optimize the fermentation medium and condition of Lactobacillus plantarum to enhance the production of phenolic compounds with the antibacterial activity tested against Propionibacterium acnes and the optimum conditions of bajakah bark fermentation using Response Surface Method (RSM) methods.

Methods: The antibacterial activity test ethanolic extract of bajakah bark was assayed using the agar diffusion perforator method and fermentation method using L. plantarum. Measurement of phenolic content fermentation and without fermentation was done using the standard methods with gallic acid as standard. The optimum conditions of bajakah bark fermentation using RSM methods using Software Design Expert 10.0.0.1.

Results: As the result, Response surface analysis revealed the optimum values of the tested significant variables for the production of phenolic were extracted in a concentration 40% (w/v) and 30% (w/v) sucrose for 3 d fermentation period. Under this optimal condition, the phenolic compounds were improved from 10.66 to 18.46 mg GAE/g extract. As well as the antibacterial activity of the fermented extract was increased by 1.35 times compared to the non-fermented extract.

Conclusion: In summary that, the optimized fermentation condition could be helpful for the production of antibacterial metabolites from bajakah bark by L. plantarum.

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References

Setianingsih S, Kartika R, Simanjuntak P. Isolation and toxicity test of stigmastan-3, 5-dien from Eucalyptus deglupta Blume. JKM. 2017;15(1):1-4.

Fitriani S, Eldha S, Suroto HS. Karakteristik tanaman akar bajakah (Spatholobus littoralis Hassk.) dari loakulu kabupaten kutai kartanegara. JRTI. 2020;14(2):365-76.

Zhang Q, Zhao JJ, Xu J, Feng F, Qu W. Medicinal uses phytochemistry and pharmacology of the genus Uncaria. J Ethnopharmacol. 2015;173:48-80. doi: 10.1016/j.jep.2015.06.011, PMID 26091967.

Nitzan O, Elias M, Peretz A, Saliba W. Role of antibiotics for treatment of inflammatory bowel disease. World J Gastroenterol. 2016;22(3):1078-87. doi: 10.3748/wjg.v22.i3.1078, PMID 26811648.

Fitz Gibbon S, Tomida S, Chiu BH, Nguyen L, Du C, Liu M. Propionibacterium acnes strain populations in the human skin microbiome associated with acne. J Invest Dermatol. 2013;133(9):2152-60. doi: 10.1038/jid.2013.21, PMID 23337890.

Jończyk Matysiak E, Weber Dąbrowska B, Zaczek M, Miedzybrodzki R, Letkiewicz S, Lusiak Szelchowska M. Prospects of phage application in the treatment of acne caused by propionibacterium acnes. Front Microbiol. 2017;8:164. doi: 10.3389/fmicb.2017.00164, PMID 28228751.

Paramita PP, Tata HL. Phytochemical compounds identification of three bajakah species (Salacia sp., Uncaria acida, and Uncaria gambir) using GC-MS pyrolysis. IOP Conf Ser.: Earth Environ Sci. 2021;762(1):1-13. doi: 10.1088/1755-1315/762/1/012043.

Ningsih AS, Ekowati CN, Sumardi FS. Uji daya antibakteri isolat bakteri asam laktat dari kefir dengan inokulum ragi tape terhadap Esscheria coli. J Biosfer. 2018;9(2):28-42.

Maajid MN, Sutjiati R, Joelijanto R. Antibacterial activity of fermented and non-fermented Edel cacao bean extract (Theobroma cacao L.) against Porphyromonas gingivalis. J Health Natl. 2021;5(8):289-94. doi: 10.33846/hn50805.

Trihaditia R, Syamsiah M, Awaliyah A. Penentuan formulasi optimum Pembuatan penambahan tepung terigu menggunakan metode rsm (response surface method). J Agro Sci. 2018;8(2):212-30.

Puspitasari AD, Wulandari RL. Aktivitas antioksidan dan penetapan Kadar flavonoid total ekstrak etil asetat daun kersen (Muntingia calabura). J Pharm Sci. 2017;4(2):167-75. doi: 10.20527/jps.v42.5770.

Misna M, Diana K. Aktivitas antibakteri ekstrak kulit bawang merah (Allium cepa L.) terhadap bakteri staphylococcus aureus. JFG 2016;2(2):138-44. doi: 10.22487/j24428744.2016.v2.i2.5990.

Saputera MM, Marpaung TW, Ayuchecaria N. Konsentrasi ham bat minimum Kadar ekstrak etanol batang bajakah tampala (Spatholobus littoralis Hassk.) terhadap bakteri Esscheria coli metode sumuran. J Manuntung Sci. 2019;5(2):167-73.

Nur A, Najib S. Skrinning fitokimia dan penetapan kadar total fenol, flavonoid, dan tannin pada daun kersen (Muntingia calabura L.). IJPHM. 2022;1(2):96-104.

Fauzia RP, Mutalib A, Soedjanaatmaja UM, Anggareni A, Yusuf M, Bahti HH. Aplikasi desain eksperimen placket-burman dan response surface methodology box-behnken pada produksi senyawa pengontras magnetic resonance imaging gadolidium dietilentriaminpenta asetat-folat. J Chem Nat Acta. 2017;5(1):1-8. doi: 10.24198/cna.5:n1.12813.

Suryani N, Nurjanah D, Indriatmoko DD. Aktivitas antibakteri ekstrak batang kecombrang (Etlingera elatior) terhadap bakteri plak gigi Streptococcus mutans. JKK. 2019;2(1):23-9. doi: 10.26874/jkk.v2i1.19.

Heitzman M, Neto C, Winiarz E, Hammond G. Ethnobotany, phytochemistry, and pharmacology of Uncaria. J Phytochem. 2004;66(1):5-29. doi: 10.1016/j.phytochem.2004.10.022.

Setiyoningrum F, Priadi G, Herlina N, Solikhin A, Lisani N. Functional properties of saccharomyces kluyveri Y97-fermented solo black garlic. Asian J Agric 2018;2(2):48-51. doi: 10.13057/asianjagric/g020203.

Rahmi N, Khairiah N, Rufida HS, Muis A. Pengaruh fermentasi terhadap total fenolik, aktivitas penghambatan radikal bebas dan aktivitas antibakteri ekstrak tepung biji Teratai (Nymphaea pubescens). J Biop Ind. 2020;11(1):9-18. doi: 10.36974/jbi.v11i1.5553.

Amalia A, Arumsari A. Studi literatur pengaruh penambahan starter fermentasi pada bawang hitam (Allium sativum) terhadap beberapa Metabolit sekunder serta aktivitas antioksidan dan antibakteri. Prosiding Farmasi. 2020;6(2):1004-10. doi: 10.29313/.v6i2.24239.

Susanty, Bachmid F, Maserasi PME, Fenolik DRT. Perbandingan metode ekstraksi maserasi dan refluks terhadap kadar fenolik dari ekstrak tongkol jagung (Zea Mays L.). J Conv. 2016;5(2):87-93. doi: https://doi.org/10.24853/konversi.5.2.87-92

Pujari V, Chandra TS. Statistical optimization of medium components for enhanced riboflavin production by a UV-mutant of Eremothecium ashbyii. Process Biochemistry. 2000;36(1-2):31-7. doi: 10.1016/S0032-9592(00)00173-4.

Published

18-12-2023

How to Cite

ALMEIDA, M., NARSA, A. C., KUSUMA, S. A. F., & SRIWIDODO, S. (2023). OPTIMIZATION OF LACTOBACILLUS PLANTARUM FERMENTATION FOR ENHANCED PHENOLIC PRODUCTION FROM EXTRACT OF BAJAKAH BARK (UNCARIA NERVOSA) BY RESPONSE SURFACE METHODOLOGY. International Journal of Applied Pharmaceutics, 15(2), 123–127. https://doi.org/10.22159/ijap.2023.v15s2.23

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