OPTIMIZATION OF KAPPA CARRAGEENAN POLYMER CONCENTRATION AND POTASSIUM CHLORIDE CROSSLINKER ON PHYSICAL CHARACTERISTICS OF GLUTATHIONE-KAPPA CARRAGEENAN NANOSPHERE

YUYUN NAILUFA; BAMBANG WIDJAJA; YULI AINUN NAJIH; DITANURLITA RAKHMA

doi:10.22159/ijap.2023.v15s2.24

Authors

YUYUN NAILUFA Pharmacy Study Program, Hang Tuah University Surabaya, Surabaya-60271, Indonesia https://orcid.org/0000-0003-0240-4626
BAMBANG WIDJAJA Pharmacy Study Program, Hang Tuah University Surabaya, Surabaya-60271, Indonesia
YULI AINUN NAJIH Pharmacy Study Program, Hang Tuah University Surabaya, Surabaya-60271, Indonesia
DITANURLITA RAKHMA Pharmacy Study Program, Hang Tuah University Surabaya, Surabaya-60271, Indonesia

DOI:

https://doi.org/10.22159/ijap.2023.v15s2.24

Keywords:

Nanosphere, Glutathione, Kappa carrageenan, Potassium chloride, Ionotropic gelation

Abstract

Objective: The objective of the study to obtain the most optimal physical characteristics of glutathione-kappa carrageenan nanosphere in terms of particle size, moisture content, yield, drug loading and entrapment efficiency.

Methods: One of the drug delivery systems that can improve drug stability for antioxidants is nanosphere. Nanospheres were prepared using ionotropic gelation method with aerosol technique. This method is easy, fast, and relatively cost-effective to manufacture. This research was applied with optimization using a randomized full factorial design of 2² with differences in kappa carrageenan as polymer and potassium chloride concentrations as crosslinker. This study aims to obtain the most optimal physical characteristics of the nanosphere in terms of particle size, moisture content, yield, drug loading and entrapment efficiency.

Results: The glutathione-kappa carrageenan nanosphere had particle sizes ranging from 247.03 to 675.07 nm with spherical and smooth nanosphere. Furthermore, the entrapment efficiency value ranged from 25.50–35.61%, and drug loading of 6.84–10.16%. The concentration of kappa polymer affected particle size, moisture content, entrapment efficiency, and drug loading. This indicated that higher polymer concentration resulted in greater particle size, moisture content, entrapment efficiency, and drug loading.

Conclusion: The most optimal formula is F4 with 1% kappa-carrageenan concentration and 0.6% KCl.

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References

Lushchak VI. Glutathione homeostasis and functions: potential targets for medical interventions. J Amino Acids. 2012;2012:736837. doi: 10.1155/2012/736837, PMID 22500213.

Allen J, Bradley RD. Effects of oral glutathione supplementation on systemic oxidative stress biomarkers in human volunteers. J Altern Complement Med. 2011;17(9):827-33. doi: 10.1089/acm.2010.0716, PMID 21875351.

Geyik G, Işıklan N. Design and fabrication of hybrid triple-responsive κ-carrageenan-based nanospheres for controlled drug delivery. Int J Biol Macromol. 2021;192:701-15. doi: 10.1016/j.ijbiomac.2021.10.007, PMID 34637816.

Dmour I, Taha MO. Natural and semisynthetic polymers in pharmaceutical nanotechnology. Org Mater Smart Nanocarriers Drug Deliv. 2018;3:5-100.

Tanusorn N, Thummarungsan N, Sangwan W, Lerdwijitjarud W, Sirivat A. Influence of carrageenan molecular structures on electromechanical behaviors of poly(3-hexylthiophene)/carrageenan conductive hydrogels. Int J Biol Macromol. 2018;118(B):2098-107. doi: 10.1016/j.ijbiomac.2018.07.066, PMID 30009911.

Rowe RC, Sheskey PJ, Quinn ME. Handbook of pharmaceutical excipients. 6th ed. Pharmaceutical Press; 2009. p. 122-5.

Hariyadi DM, Ma Y, Wang Y, Bostrom T, Malouf J, Turner MS. The potential for production of freeze-dried oral vaccines using alginate hydrogel microspheres as protein carriers. J Drug Deliv Sci Technol. 2014;24(2):178-84. doi: 10.1016/S1773-2247(14)50029-9.

Hariyadi DM, Hendradi E, Purwanti T, Fadil FDGP, Ramadani CN. Effect of crosslinking agent and polymer on the characteristics of ovalbumin loaded alginate microspheres. Int J Pharm Pharm Sci. 2014;6(4):69-474.

Hariyadi CN, Purwanti T, Adilla S. Influence of crosslinker concentration on the characteristics of erythropoietin-alginate microspheres. J Pharm Pharmacogn Res. 2018;6(4):250-9.

Tecante A, Nunezsantiago NC. Rheologi: solution properties of κ-carrageenan and its interaction with other polysaccharides in aqueous media. InTech. 2012.

Hariyadi DM, Noorma R, Rahayu A. Design, optimization and characterization of glutathione loaded alginate microspheres for topical antiaging. J Pharm Pharmacogn Res. 2019;7(4):223-33.

Jung H, Lee YJ, Yoon WB. Effect of moisture content on the grinding process and powder properties in food: a review. Processes. 2018;6(6):69. doi: 10.3390/pr6060069.