VALIDATED REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE ESTIMATION OF TETRABENAZINE IN SELF-NANO EMULSIFYING DRUG DELIVERY SYSTEMS

SHASHI; NARENDRA KUMAR PANDEY; BIMLESH KUMAR; SACHIN KUMAR SINGH; DILEEP SINGH BAGHEL; KALVATALA SUDHAKAR; SAURABH SINGH

doi:10.22159/ijap.2024v16i5.50489

Authors

SHASHI School of Pharmaceutical Sciences, Lovely Professional University, Punjab-144411, India https://orcid.org/0000-0002-4203-1476
NARENDRA KUMAR PANDEY School of Pharmaceutical Sciences, Lovely Professional University, Punjab-144411, India https://orcid.org/0000-0003-0821-7653
BIMLESH KUMAR School of Pharmaceutical Sciences, Lovely Professional University, Punjab-144411, India https://orcid.org/0000-0001-8072-5172
SACHIN KUMAR SINGH School of Pharmaceutical Sciences, Lovely Professional University, Punjab-144411, India https://orcid.org/0000-0003-3823-6572
DILEEP SINGH BAGHEL School of Pharmaceutical Sciences, Lovely Professional University, Punjab-144411, India
KALVATALA SUDHAKAR School of Pharmaceutical Sciences, Lovely Professional University, Punjab-144411, India https://orcid.org/0000-0002-1123-9864
SAURABH SINGH School of Pharmaceutical Sciences, Lovely Professional University, Punjab-144411, India

DOI:

https://doi.org/10.22159/ijap.2024v16i5.50489

Keywords:

Tetrabenazine, HPLC, Analytical method development, SNEDDS

Abstract

Objective: Self-Nano-Emulsifying Drug Delivery System (SNEDDS) of tetrabenazine (TBZ) was analysed using reverse-phase high-performance liquid chromatography.

Methods: Optimized chromatographic condition was consisted of Acetonitrile (ACN) and 0.1% v/v formic acid in the ratio of 90:10 as a mobile phase in isocratic mode at 25±1 °C. In this C-18 (250 mm×4.6 mm, 5 µm) column was used and absorbance was recorded at 283 nm.

Results: The compound was eluted at a flow rate of 1.0 ml/min and retention time (RT) was observed as 4.34±0.03 min. TBZ showed linearity over 2-10 µg/ml conc. and the value of regression was obtained as 0.9992. The developed method was found precise due to Percentage Relative Standard Deviation (%RSD) was less than 2 %. On the other hand, 0.31 and 0.96 were investigated value for Limit of Detection (LOD) and Limit of Quantification (LOQ), respectively.

Conclusion: The method adopted was found to be robust and can be apply for the determination of drug in different oil, surfactants and co-surfactants for the calculation of drug loading of pharmaceutical product formulation.

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