RETINOL STABILITY IN ANTI-AGING FACE SERUM POST-OPENING PERIOD
DOI:
https://doi.org/10.22159/ijap.2024v16i4.50903Keywords:
Retinol, Face serum, HPLC, StabilityAbstract
Objective: This study aimed to evaluate the stability of retinol in facial serum products under various storage conditions during a specific period after the product is opened. Retinol analysis will be conducted using reversed phase high performance liquid chromatography with a UV detector.
Methods: The optimum analytical method in this study was validated according to the ICH Q2 (R1) guideline. The chromatographic separation of retinol was achieved on RP-HPLC isocratic elution method at a wavelength of 324 nm, C18 column, mobile phase of methanol-acetonitrile (90:10), and a flow rate of 0.8 ml/min.
Results: The recovery value for this method was 98.06% - 101.6,6%, with RSD ≤ 2%. Limit of Detection (LOD) and Limit of Quantification (LOQ) values obtained were 1.1819 µg/ml and 3.9399 µg/ml. Determining retinol levels in face serum samples with variation in storage conditions was conducted on days 0, 7, 14, 21, and 30. The retinol levels in all storage conditions decreased over the 30 days, ranging from 25% to 79%. The highest decrease was observed in samples stored in transparent containers and exposed to light, with a reduction of 79% and an average weekly decrease of 17%.
Conclusion: Stability evaluation of retinol in the serum formulation showed decreased retinol concentration over time during storage. Transferring the product to a secondary container and exposure to light resulted in a higher concentration decrease than storing it in the original bottle.
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