a COMBINING LIQUID CHROMATOGRAPHY WITH TANDEM MASS SPECTROMETRY (LC-MS/MS) TECHNIQUE, SPEKTROFOTOMETER VALIDATION AND AN IN SILICO STUDY OF 96% ETHANOL EXTRACT OF SPIRULINA PLATENSIS
COMBINING LIQUID CHROMATOGRAPHY WITH TANDEM MASS SPECTROMETRY (LC-MS/MS) TECHNIQUE, SPEKTROFOTOMETER VALIDATION AND AN IN SILICO STUDY OF 96% ETHANOL EXTRACT OF SPIRULINA PLATENSIS
DOI:
https://doi.org/10.22159/ijap.2024v16i5.51339Keywords:
C-phycocyanin, in silico study, LC-MS/MS, spektrophotometer, Spirulina platensisAbstract
Objective: This study was to analyze the components of a 96% ethanol extract of Spirulina platensis by the LC-MS/MS technique, then validated them with the spectrophotometer technique using the C-phycocyanin standard and an in silico study approach as an antioxidant property of Spirulina platensis against inflammatory.
Methods: Chromatographic resolution was attained with a PhenominexC18 (50 mm×2.6 mm, 3 µm) stationary column technique, validation using C-phycocyanin standar using the spektrophotometer technique, and an in silico study of C-phycocyanin using molecular docking analysis.
Results: Tentative active compounds such as Flavonoid (Maltol and Morin), Peptide (Cyclo Pro-Ala, Cyclo Pro-Pro, and Thymine), and Phenol (m-Aminophenol, N-Methyltyramine, and Tyramine) have been identified from a 96% ethanol extract of Spirulina platensis by LCMS/MS analysis. The concentration of C-phycocyanin in the 96% ethanol extract of Spirulina platensis is 229,2µg/ml. C-phycocyanin has the potential to prevent the activity of anti-inflammatory agents in silico studies.
Conclusion: The LC-MS/MS technique can detect Flavonoid, Peptide, and Phenolic components in the 96% ethanol extract of Spirulina platensis. A Spektrophotometer can identify the validation equation of C-phycocyanin in a 96% ethanol extract of Spirulina platensis. C-phycocyanin has been shown in an in silico study to possess anti-inflammatory properties.
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