COMBINED CHIP APPLICATION'S EFFECT OF CLINICAL PARAMETERS AND TNF-α EXPRESSIONS
DOI:
https://doi.org/10.22159/ijap.2024.v16s2.12Keywords:
Periodontitis, Periodontal chip, Clinical parameters, TNF-αAbstract
Objective: Scaling root planing is the main mechanical treatment to remove local deposits. Supportive local and systemic antimicrobial therapy is used to eliminate or reduce the number of pathogenic bacteria. The chip application of the combination of snakehead fish extract and betel leaf can change the clinical parameters for the better and increase the expression of TNF-α as a pro-inflammatory. To see the effect of the application of a chip combination of snakehead fish extract and betel leaf in male Wistar rats induced by periodontitis as a support for initial therapy on clinical parameters and expression of TNF-α.
Methods: Laboratory experimental study with a posttest control group design in which 24 male Wistar rats induced periodontitis were in four treatment groups (2.5%, 5%, 10% snakehead fish extract and placebo). Examination of clinical parameters and expression of TNF-α was carried out on day 0, day 3 and day 7. Data were analyzed by the Mann-Whitney test.
Results: The results showed that the clinical parameters; gingival index, pocket depth and attachment level looked better on the 7th day after treatment with statistically significant results (p≤0.05) and the highest anti-inflammatory TNF-α expression occurred at a concentration of 5%.
Conclusion: The application of a chip combination of snakehead fish extract and betel leaf as a support for initial therapy is effective in reducing the gingival index pocket depth and increasing the level of attachment, which is statistically significant, but there is no significant difference in TNF-α expression.
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References
Yucel T. Lindberg, Bage T, inflammatory mediators in the pathogenesis of periodontitis. Expert review Swedia; 2013. Available from: http://www.expertreviews.org/.2013
Eivazi M, Falahi N, Eivazi N, Eivazi MA, Raygani AV, Rezaei F. The effect of scaling and root planning on salivary TNF-α and IL-1α concentrations in patients with chronic periodontitis. Open Dent J. 2017;11:573-80. doi: 10.2174/1874210601711010573, PMID 29238418.
Assuma R, Oates T, Cochran D, Amar S, Graves DT. IL-1 and TNF antagonists inhibit the inflammatory response and bone loss in experimental periodontitis. J Immunol. 1998;160(1):403-9. doi: 10.4049/jimmunol.160.1.403, PMID 9551997.
Bratawidjaja KG, Rengganis I, Dasar I, FKUI. Jakarta. Edisi. 2018;XII:205-59, 263-97.
Shita AD. Pengaruh gaya ortodonsi terhadap ekspresi [Tumor necrosis factor-α] tulang alveolar pada model tikus diabetes yang diinduksi streptozotocin (Kajian in vivo pada tikus wistar). Laporan Hasil Penelitian Hibah Khusus Bagi Peneliti Muda atau Pemula. Jember: Universitas Jember; 2015. p. 3.
Kartini DS, Analisis Kadar. Tumor necrosis factor alpha (TNF_α) cairan bilasan bronkus pada pasien kanker paru. Universitas Hasanuddin. Makasar; 2018.
Newman MG, Takei HH, Klokkenvold PR, Carranza FA. Newman and carranza’s clinical periodontology. New York. 13th ed. Elsevier; 2015. p. 149-466.
Jhinger N, Kapoor D, Jain R. Comparison of PerioChip (chlorhexidine gluconate 2.5 mg) and Arestin (minocycline hydrochloride 1 mg) in the management of chronic periodontitis. Indian J Dent. 2015;6(1):20-6. doi: 10.4103/0975-962X.151697, PMID 25767356.
Medaiah S, Srinivas M, Melath A, Girish S, Polepalle T, Dasari AB. Chlorhexidine chip in the treatment of chronic periodontitis-a clinical study. J Clin Diagn Res. 2014;8(6):ZC22-5. doi: 10.7860/JCDR/2014/8808.4477, PMID 25121059.
Seo J, Lee U, Seo S, Wibowo AE, Pongtuluran OB, Lee KJ. Anti-inflammatory and antioxidant activities of methanol extract of piper betle linn. (Piper betle L.) leaves and stems by inhibiting NF-κB/MAPK/Nrf2 signaling pathways in RAW 264.7 macrophages. Biomed Pharmacother. 2022;155:(113734):1-11.
Andini A, Prayekti E. Chitosan as antifungal in Channa striata collagen-chitosan for wound healing. J Med Health Sci. 2019;3(2):8-11.
Ichwana DL, Nasroen SL. Parameter jaringan periodontal pada anak-anak dan remaja dengan celah bibir dan langit-langit. Prosiding Seminar Nasional Ilmu Pengetahuan & Teknologi Jenderal Achmad Yani (SNIJA); 2017. p. 71-4.
Tyagi P, Dodwad V, Kukreja BJ, Kukreja P. A comparison of the efficacy of scaling and root planning with application of pomegranate chip, pomegranate gel, and scaling and root planing in sufferers with adult periodontitis-a prospective study. J Indian Soc Periodontol. 2021;25(1):41-6. doi: 10.4103/jisp.jisp_243_20, PMID 33642740.
Octavia M, Soeroso Y, Kemal Y. Clinical effect after scaling and root planing in chronic periodontitis with 4-6 mm deep pocket. Dentika Dent J. 2015;18(3):211-17.
Karyadi E, Syaifyi A. Ekspresi kadar [Tumor necrosis factor-α (TNF-α)]. Cairan sulkus gingiva pada penderita gingivitis [Kajian Pengguna Kontrasepsi Pil Suntik Implan]. JIKG. 2019;2(1):1-5.
Sidiqa AN, Herryawan, Sirih Merah. EGD (Piper crocatum) pada perawatan periodontitis kronis. Kartika-Jurnal Ilmiah Farmasi. 2017;5(1):1-6.
Achmad H, Thahir H, Rieuwpassa I, Mardiana AA, Oktawati S, Samad R. The effectiveness of Channa striata extract antimicrobial effect on periopathogen bacteria (Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans). Syst Rev Pharm. 2020;11(4):319-23.
Andriani I. Effectiveness scaling root planing (Srp) with and without ciprofloxacin for periodontitis. IDJ. 2012;1(2):81-90.
Polonczyk JP, Jakubik A, Bierowiec P, Muszynska B. Artificial saliva and its use in biological experiments. J Physiol Pharmacol. 2017 Dec;68(6):807-13. PMID: 29550792
Achmad H, Adam AM, Thahir H. Effect of Channa striata extract on the expression of tumor necrosis factor-α (TNF-α) in rat experience periodontitis. Indian J Public Health Res Dev. 2019;10(9):139.
Mohamad Isa II, Abu Bakar S, Md Tohid SF, Mat Jais AM. Channa striatus cream down-regulates tumour necrosis factor (TNF)-alpha gene expression and alleviates chronic-like dermatitis in mouse model. J Ethnopharmacol. 2016;194:469-74. doi: 10.1016/j.jep.2016.10.033, PMID 27732902.
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