EFFECTIVENESS OF CHITOSAN-LOADED TETRACYCLINE IN PERIODONTITIS INDUCED RATS
DOI:
https://doi.org/10.22159/ijap.2024.v16s2.14Keywords:
Periodontal disease, Tetracycline, Hydrogel, Chitosan, CollagenAbstract
Objective: The primary treatments for periodontal disease are scaling and Root Surface Debridement (SRSD), which involves eliminating bacteria plaque and achieving a smooth root surface area. Tetracycline helps increase attachment and enhances the regeneration of alveolar bone and tissue. Chitosan is an adequate system for local drug preparation in periodontal pockets. The purpose of this study was to evaluate the effectiveness of chitosan tetracycline hydrogel in pocket depth and collagen density in periodontal ligaments in animal models.
Methods: Three groups of Wistar rats were categorized. The first group treatment was SRSD with tetracycline 0.7% based chitosan hydrogel for seven days; the second group was SRSD with an application of tetracycline 0.7% based chitosan hydrogel on the first day, and the third group treatment was SRSD only. After treatment, pocket depth was evaluated at the baseline and on the 14th day, along with the examination of collagen tissue density in the periodontal ligament, which appeared as blue fibers with Masson’s trichrome staining, was seen and compared to other groups.
Results: The most significant change in the pocket depth was observed in this study, along with improved collagen tissue density, was found in the group of SRSD with an application of tetracycline 0.7% based chitosan hydrogel for seven days, and this result was statistically significant (p<0.05) compared to other groups.
Conclusion: Tetracycline 0.7% based chitosan hydrogel for seven days shows a better result in decreasing pocket depth and increasing collagen tissue density in periodontal ligaments.
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References
Carranza Fermin A, Takei Henry H. The treatment plan. In: Carranza’s clinical periodontology. 12th ed. Philadelphia: Saunders - Elsevier; 2015. p. 125-6.
Bathla S, Bathla M. Periodontics revisited. New Delhi: Jaypee Brother; 2011. p. 72-5.
Vyas M, Vyas S. Assessment of tetracycline as an adjunct to scaling and root planing in periodontal patients. Int J Contemp Med Rad. 2018;3(1):126-8.
Meharwade VV, Gayathri GV, Mehta DS. Effects of scaling and root planing with or without a local drug delivery system on the gingival crevicular fluid leptin level in chronic periodontitis patients: a clinical-biochemical study. J Periodontal Implant Sci. 2014;44(3):118-25. doi: 10.5051/jpis.2014.44.3.118.
Rangaraj A, Harding K, Leaper D. Role of collagen in wound management. Wound UK. 2011;7(2):54-60.
Balogh, Fehrenbach MB, Dental MJ. Embryology, histology and anatomy. 2nd ed. Amsterdam: Elsevier Inc.; 2006. p. 112-3.
Pradnyani IG. Tetrasiklin HCl gel 0,7% meningkatkan jumlah sel fibroblast dan mempertebal ligamen periodontal pada sulkus gingiva tikus yang mengalami periodontitis. DOAJ. 2017;8(1):14-8.
Pejcic A, Kesic L, Obradovic R, Mircovic D. Antibiotic in the management of periodontal disease. SJFM. 2010;27(2):86-8.
Alvarenga ES. Characterization and properties of chitosan. Biotechnol Biopolym; 2014. p. 92-107.
Popa L, Ghica MV, Pirvu CE. Periodontal chitosan-gels designed for improved local intra-pocket drug delivery. Farmacia. 2013;61(2):240-51.
Susanto C, Ervina I, Agusnar H. In vitro evaluation of antimicrobial effectiveness, chitosan-based tetracycline gel on some pathogenic periodontal bacteria. IJADS. 2017;3(2):71-6.
Andrew EI, Agusnar H. In vitro chitosan hydrogel-based tetracycline cytotoxicity test on fibroblast viability. IDSM. 2017;4:265-6.
Kafle S, Pradhan S, Gupta S. Locally delivered tetracycline fibres in the treatment of chronic periodontitis. J Nepal Soc Perio Oral Implantology. 2018;2(2):45-8. doi: 10.3126/jnspoi.v2i2.23612.
Babrawala IS, Mlv P, Bv K, Khanna D. A novel approach using natural 1% (W/W) chitosan as a local drug delivery system in the management of nonsurgical periodontal treatment: a pilot study. J Int Acad Periodontol. 2016;18(4):129-33. PMID 31473701.
Sadaf N, Anoop B, Dakshina B, Shweta B. Evaluation of efficacy of tetracycline fibers in conjunction with scaling and root planing in patients with chronic periodontitis. J Indian Soc Periodontol. 2012;16(3):392-7. doi: 10.4103/0972-124X.100918, PMID 23162335.
Darmastuti W. Pengaruh tetrasiklin hidroklorid 75 mg/ml dengan pelarut akuades pasca kuretase terhadap kepadatan sera but kolagen pada proses regenerasi model periodontitis kelinci; 2019. Available from: http://etd.repository.ugm.ac.id [Last accessed on 07 Nov 2019]
Fernandes LA, Martins TM, Almeida JM, Nagata MJ, Theodoro LH, Garcia VG. Experimental periodontal disease treatment by subgingival irrigation with tetracycline hydrochloride in rats. J Appl Oral Sci. 2010;18(6):635-40. doi: 10.1590/s1678-77572010000600017, PMID 21308297.
Gomes JR, Omar NF, Neves JD, Novaes PD. Doxycycline reduces the expression and activity of matrix metalloproteinase-2 in the periodontal ligament of the rat incisor without altering the eruption process. J Periodontal Res. 2017;52(3):353-9. doi: 10.1111/jre.12398, PMID 27417412.
Pang EK, Paik JW, Kim SK, Jung UW, Kim CS, Cho KS. Effects of chitosan on human periodontal ligament fibroblasts in vitro and on bone formation in rat calvarial defects. J Periodontol. 2005;76(9):1526-33. doi: 10.1902/jop.2005.76.9.1526, PMID 16171443.
Fatimah S, Geetah A, Mahendra J, Ambalavanan N. Gingival crevicular fluids levels of N-telopeptide of type-1 collagen before and after nonsurgical periodontal therapy in generalized chronic periodontitis subjects. IJRSR. 2017;8(2):15550-3.
Octavia M, Soeroso Y, Kemal Y. Efek klinis setelah skeling dan penghalusan akar kasus periodontitis kronis poket 4-6 mm. Dentika Dental J. 2015;18(3):211-7. doi: 10.32734/dentika.v18i3.1951.
Choi YM, Lee JY, Choi J, Joo JY. Effect of root planing on the reduction of probing depth and the gain of clinical attachment depending on the mode of interproximal bone resorption. J Periodontal Implant Sci. 2015;45(5):184-9. doi: 10.5051/jpis.2015.45.5.184, PMID 26550527.
Andriani I. Efektivitas antara scaling root planing (SRP) dengan dan tanpa pemberian ciprofloxacin per oral pada penderita periodontitis. Insisiva Dent J. 2012;1(2):1-6.
Novitasari AI, Indraswary R, Pratiwi R. Pengaruh aplikasi gel ekstrak membrane kulit telur bebek 10% terhadap kepadatan sera but kolagen pada proses penyembuhan luka gingiva. Odonto Dent J. 2017;4(1):13-20.
Natalina SI, Yuniarti. Efek irigasi tunggal larutan tetrasiklin HCl 10% setelah skeling dan penghalusan akar terhadap perubahan klinis periodontitis kronis poket 4-6 mm. JKGUI. 2003;10:585-90.
Sinha S, Kumar S, Dagli N, Dagli RJ. Effect of tetracycline HCl in the treatment of chronic periodontitis-a clinical study. J Int Soc Prev Community Dent. 2014;4(3):149-53. doi: 10.4103/2231-0762.142011, PMID 25374831.
Häkkinen L, Uitto VJ, Larjava H. Cell biology of gingival wound healing. Periodontol 2000. 2000;24:127-52. doi: 10.1034/j.1600-0757.2000.2240107.x, PMID 11276865.
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Copyright (c) 2024 WINDA DWI ASTUTI, IRMA ERVINA, WILSON, MARTINA AMALIA, ARMIA SYAHPUTRA, RINI OCTAVIA NASUTION, HARRY AGUSNAR, SYAFRUDDIN ILYAS
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