ANALYSIS OF PROPOLIS STINGLESS BEE BIOACTIVE COMPOUNDS FROM SEVERAL REGIONS IN INDONESIA

DIAH KARTIKA PRATAMI; SANIA CITRA ALFIFAH; IZZUL ISLAM; MUHAMAD SAHLAN; SRI ANGKY SOEKANTO

doi:10.22159/ijap.2024v16i3.14

Authors

DIAH KARTIKA PRATAMI Lab of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta-12640, Indonesia. Center for Study of Natural Product for Degenerative Disease, Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta-12640, Indonesia https://orcid.org/0000-0003-1052-5946
SANIA CITRA ALFIFAH Faculty of life Sciences and Technology, Sumbawa University of Technology, Sumbawa, West Nusa Tenggara-84371, Indonesia
IZZUL ISLAM Faculty of life Sciences and Technology, Sumbawa University of Technology, Sumbawa, West Nusa Tenggara-84371, Indonesia
MUHAMAD SAHLAN Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java-16424, Indonesia. Research Center for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java-16424, Indonesia
SRI ANGKY SOEKANTO Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Central Jakarta, DKI Jakarta-10430, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024v16i3.14

Keywords:

Stingless bee, Propolis, lC-MS/MS, Phenolic, Flavonoid, Antioxidant

Abstract

Objective: Propolis is a bee product collected by honeybees from a resinous substance of various plant sources. Its antioxidant activities are different from various geographic origins. This study aimed to analyze bioactive compounds by lC-MS/MS, compare antioxidant activity, total phenolic and flavonoid contents in stingless bee propolis samples from several regions in Indonesia.

Methods: The propolis samples were taken from stingless bee hives of Tetragonula clypearis from Sumbawa, Tetragonula laeviceps from Magelang, Tetragonula biroi from Bogor, and Geniotrigona thoracica from South Kalimantan). Analysis of bioactive compounds was identified by lC-MS/MS. The quantification of the chemical compound determined its total phenolics and flavonoid (TPC and TFC) contents. The antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay.

Results: The results of this study obtained 10 compounds from G. thoracica, 8 compounds each from T. laeviceps and T. biroi, and 7 compounds from T. clypearis. The results of TPC was ranging from 57.24±5.35 to 139.39±15.79 mg GAE/g. The TFC was ranging from 22.13±0.79 to 37.20±3.63 mg QE/g. With the highest TPC and TFC was propolis of G. thoracica. The IC₅₀ of antioxidant activity was ranging from 11.12 to 162.72 ppm.

Conclusion: The compounds contained in propolis T. clypearis, T. laeviceps, G. thoracica and T. biroi have a potential as a new herbal candidate as antioxidant agents.

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