OPTIMIZATION OF LIPASE PRODUCTION FROM DIFFERENT AGROINDUSTRIAL WASTES BY MARINE ACTINOMYCETES
Keywords:
Marine Actinomycetes, Lipase, Coconut cake, Groundnut cakeAbstract
Objective: To identify the production of extracellular lipase by solid state fermentation (SSF) using coconut cake and groundnut cake using thermophilic strain of marine actinomycetes.
Methods: From the isolated strains of marine actinomycetes a comparative study has been evaluated on different intends such as pH, temperature, carbon sources, nitrogen sources and additive sources were optimized for maximum yield.
Results: Five different pH was used from 5 to 9 and on the other hand 3 different temperatures were maintained, sucrose, fructose and dextrose were used as carbon sources while urea, peptone and Ammonium nitrate was used as nitrogen sources similarly Tween 20 was used as additive sources. On comparing coconut oil cake and groundnut oil cake for all these different intends, there was maximum extracellular lipase specific activity was obtained in groundnut oil cake.
Downloads
References
Maritinelle M, Holmquist M, Hult K. On the interfacial activation of candida Antarctica lipase A and B as compared with Humicola lanuginose lipase. Biochem Biophy Acta 1995;4:272-6.
Ito TK, Nagamori HE, Honda H, Ogino H, Ishikawa H, kobayashi T, et al. Lipase production in two step fed batch culture of organic solvent tolerant Pseudomonas aeruginosa LST-03. J Biosci Bioen 2001;23:245-50.
Chisti Y. Solid substrate fermentations, Enzyme production, Food enrichment. Encyclopedia of bioprocess technology: Fermentation, Biocatalysts and Bioseparation. Wiley, New York; 1995;5:2446-62.
Pandey A, Selvakumar P, Soccol CR, Nigam P. Solid state fermentation for the production of industrial enzymes. Curr Sci 1999;77:149-62.
Benjamin S, Pandey A. Mixed solid substrate fermentation: a novel process for enhanced lipase production by Candida rugosa. Act Biotech 1997;18:315-24.
Ramachandran S, Patel AK, Nampoothiri KM, Francis F, Nagy V, Szakacs G, et al. Coconut oil cake-a potential raw material for the production of amylase. Bioresour Techno 2004;93:169-74.
Rathi P, Goswami VK, Sahai V, Gupta R. Stastical medium optimization and production of a thermo stable lipase from Burkholderia cepapcia in a bioreactor. J App Micro 2002;93:930-45.
Nahas E. Control of lipase production by Rhizopus oligosporus under various growth conditions. Gen Microbial 1998;27:227-38.
Ramachandran S, Singh SK, Larroche C, Soccol CR, Pandey A. Oil cakes and their biotechnological applications. Biores Technol 2006;98:2000-9.
Sangeetha R, Geetha A, Arulpandi I. Production of protease and lipase by bacillus licheniformis vsgi: production, purification and characterization. Braz J Microbial 2010;41:179-85.
Kamini NR, Mala JGS, Puvankrishnan. Lipase production from Aspergillus niger by solid state fermentation using gingely oil cake. Pro Biochem 1998;33:505-11.
Li H, Zhang X. Characterization of thermo stable lipase from themophilic Geobacillus species. TwI Prot Express Purific 2005;42:153-9.
Mahler GF, Kok RG, Cordenons A, Hellingner KJ, Nudel BC. Effects of carbon sources on extracellular lipase production and lipA transcription in Acinetobacter calcoaeticus. J Indus Microbio Biotech 2000;24:25-30.
Gutara M, Godoy M, Maugeri F, Isabel M, Freire D, Castilho R. Production of an acidic and thermo stable lipase of the thermophilic actinomycetes by solid-state fermentation. Biores Technol 2009;21:5249-54.
Moreira KA, Albuquerque BF, Teixeira MFS, Porto ALF, Filho Lima JL. () Application of proteases from Nocardiopsis sp. as a laundry detergent additive. W J Micro Biotech 2002;18:307-12.
Gunalakshmi B, Sahu M, Sivakumar K, Thangaradjou T, Sudha S, Kanan L, et al. Investigation on lipase producing actinomycete Strain LE-1, isolated from shrimp pond. Res J Microbio 2008;8:73-81.
Alkan H, Baysal Z, Uyar F, Dogru M. Production of lipase by a newly isolated Bacillus coagulans under Solid-State Fermentation using melon wastes. Appl Biochem Biotechnol 2007;136:183-92.
Mahanta N, Gupta A, Khare SK. Production of protease and lipase by solvent tolerant Pseudomonas aeruginosa Pse A in solid-state fermentation using Jatropha curcas seed cake as substrate. Biores Technol 2008;99:1729-35.
Rodriguez JA, Mateos JC, Nungaray J, Gonzalez V, Bhagnagar T. Improving lipase production by nutrient source modification using Rhizopus homothallicus cultured in solid state fermentation. Pro Biochem 2006;41:2264-9.
Yadav RP. Lipase production by aspergillus and pencillium species. Folia Microbial 1998;43:373-8.
Guoping W. Fermentation and purification of lipase from candida rugosa, zhongguo. Yiyao Gougye Zazhi 1990;21:145-50.
Rao PV, Jayaraman K, Lakshmanan CM. Production of lipase by Candida rugosa in solid state fermentation. 2: medium optimization and effect of aeration. Process Biochem 1993;28:385-8.
Lima VMG, Krieger N, Sarquis MIM, Mitchell DA, Ramos LP, Fontana JD, et al. Effect of nitrogen and carbon sources on lipase production by Penicillium aurantiogriseum. Food Techno Biotechnol 2003;41:105-10.