SPECTROSCOPIC ANALYSIS OF BIOACTIVE COMPOUNDS FROM STREPTOMYCES CAVOURESIS KUV39: EVALUATION OF ANTIOXIDANT AND CYTOTOXICITY ACTIVITY
Keywords:
Anticancer, Antioxidant, Bioactive compound, Streptomyces cavouresis KU-V39, FT-IR and GC-MSAbstract
Objective: To assess the antioxidant and anticancer activity of crude ethyl acetate extract from Streptomyces cavouresis KU-V39 isolated from vermicompost.
Methods: To determine its antioxidant activity by total phenol and flavonoid content, Hydrogen peroxide radical scavenging activity and Ferrous reducing power assay. To determine in vitro cytotoxicity, various concentrations of extract was tested on HeLa cell line by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Fourier transform infrared spectroscopy (FT-IR) and Gas chromatography–mass spectroscopy (GC-MS) have been carried out to investigate the bioactive compound of crude extract.
Results: The Phenol and flavonoid content of crude extract was found to be 20.24 and 26.60 mg /g metabolite. Isolate KU-V39 exhibited H2O2 scavenging activity at IC50 value is 42.35 ± 0.75 μg/ml. The ferrous reducing power assay has higher absorbance value which indicates the high antioxidant capacity of the extracts. The analysis of FT-IR spectroscopy indicate presents of Phenol, carboxylic acid, alkanes, nitro and aliphatic amines functional groups. The GC – MS analysis revealed that presence of Pentanoic acid, L-Proline, Lysine, Erucic acid, isosteviol, Pentadecanoic acid, Phthalic acid, Hexadecanoic acid, Octadecenoic acid, Dichloroacetic acid, Cyclohexanecarboxylic acid and Pyrrolo pyrazine derivative.
Conclusion: This spectral study clearly proved that the vermicompost derived Streptomyces with extra cellular metabolism showed promising antioxidant and anticancer activity.
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References
Ali SS, Kasoju N, Luthra A, Singh A, Sharanabasava H, Indian S, et al. herbs as sources of Antioxidants. J Food Res Int 2008;41:1-15.
L. Arnold Sergio Sanchez. Microbial drug discovery 80 years of progress. J of Antibiotics 2009;62:5-16.
Augustine SK, Bhavsar SP, Baserisalehi M, Kapadnis BP. Isolation, characterization and optimization of antifungal activity of an actinomycete of soil origin. Indian J Exp Biol 2004;42(9):928-32.
Balachandran C, Sundaram R, Duraipandiyan V, Ignacimuthu S. Lakshmi Antimicrobial activity of Streptomyces sp. ERICPDA1 isolated from oil contaminated soil from chennai India Asian pacific. J of Tropical Biomedicine 2012:1-4.
Chang C, Swaan PW, J. Computational approaches to modeling drug transporters. J Eur Sci 2005;27:411-24.
Kerr JF, Wyllie AH, Currie AR. Apoptosis:a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 1972;26(4):239-57.
Kpuaka AA, Ekwenchi MM, Dashak DA, Dildar A, Indica A. Biological activities of characterized isolates of n-hexane extract of Azadirachta Juss leaves. J Nature and science 2013;11(5).
Narendhran S, Rajiv P, Vanathi P, Rajeshwari Sivaraj. Isolation and characterization of actinomycetes from vermicompost and it antimicrobial activity. Int J Pharmacol Bio Sci 2014;8 (2), 23-30.
Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod 2007;70(3):461-77.
Ankita K, J. Nitin Rupesh Dudhe. Synthesis of 34 5dihydro1phenyl5substituted phenyl 1 Hpyrazol 3yl 2H chromen 2 one derivatives and evaluation of their anticancer activity. J Arabian 2013:67-78.
Ordonez AA, Gomez JG, Vattuone MA, Isla MI. Antioxidant activities of Sechium edule swart extracts. J Food Chem 2006;97:452-8.
Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Japanese J of Nutrition 1986;44:307-15.
Preeti Dharmik G, Ashok Gomashe V. Isolation, identification and antioxidant activity of melanin pigment from actinomycete (Streptomyces Species) isolated from Garden Soil, Nagpur District, India. Int J Pure Appl Sci Technol 2013;18 (1) 69-72.
Sudha srikesavan S, Masilamani selvam M. Actinomycetes from marine sediment Screening for cytotoxicity identification and analysis of bioactive constituents by GCMS ICBBHS 2012:32-45.
Suthindhiran K, Kannabiran K, J. glucosidase and α – amylase inhibitory activity of Micromonospora sp. VITSDK3 EU551238. J Int Biol 115. 2009;6.
Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J of Agricultural Food Chem 1998;46:4113-7.
Kessler M, Ubeaud G, Jung L. Anti-and pro-oxidant activity of rutin and quercetin derivatives. The J of pharmacy and pharmacology 2003;55(1):131-42.