GREEN SYNTHESIS OF SILVER NANOPARTICLE FROM LEAF EXTRACT OF AEGLE MARMELOS AND EVALUATION OF ITS ANTIBACTERIAL ACTIVITY
Keywords:
Nanoparticles, Aegle marmelos, Characterization, antibacterial activityAbstract
Objective: The synthesis of metal nanoparticle is a growing area of research in modern material science and technology. Utilization of the silver nanoparticles in the field of biomedical nanotechnology and nanomedicines is rapidly growing because of their antimicrobial, anticancer, antioxidant property and less toxicity. Nanoparticles are synthesized by chemical methods, but are not eco-friendly. The objective of the study is to develop a fast, eco-friendly and convenient method for silver nanoparticle synthesis.
Methods: In this method utilization of the reducingproperty of Aegle marmelos leaf extract was done for synthesis of stable silver nanoparticles. Characterization of the metal nanoparticles was carried out by UV- Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), X-ray Diffraction microscopy (XRD), Energy Dispersive X-ray spectroscopy (EDX) and zeta potential analysis.
Results: This result showed the average particle size of 15 -30 nm and spherical structure of stable silver nanoparticles. Green synthesized nanoparticles tested for its antibacterial activity by the well diffusion method. Silver nanoparticles had shown a more inhibitory effect against Streptococcus pyogenes, Escherichia coli, and Pseudomonas aeruginosa than Staphylococcus aureus and Aeromonas hydrophila at 25, 50 and 100 µg/ml concentrations.
Conclusion: This study is recommends the use of Aegle marmelos leaves for the synthesis of silver nanoparticles and can be applied as an antimicrobial agent.
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