NOVEL TRANSETHOSOME CONTAINING GREEN TEA (CAMELLIA SINENSIS L. KUNTZE) LEAF EXTRACT FOR ENHANCED SKIN DELIVERY OF EPIGALLOCATECHIN GALLATE: FORMULATION AND IN VITRO PENETRATION TEST

Authors

  • Effionora Anwar Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia
  • Delly Ramadon Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia
  • Ghina Desviyanti Ardi Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia

DOI:

https://doi.org/10.22159/ijap.2018.v10s1.66

Keywords:

Cream, Epigallocatechin gallate, Green tea leaf extract, Penetration, Transethosome

Abstract

Objective: This study aimed to formulate a transethosome cream (TEC) to increase skin penetration of epigallocatechin gallate (EGCG) in green tea
leaf extract and evaluate their physicochemical characteristics and skin penetration capacity.
Methods: Transethosomes were prepared through thin-layer hydration method in three formulations (F1−F3) with different Span 80 and ethanol concentrations.
Transethosome morphology was characterized using transmission electron microscopy, particle size, polydispersity (PDI), and zeta potential using a particle
size analyzer and entrapment efficiency (EE). Penetration was tested using an in vitro Franz diffusion cell with female Sprague-Dawley rat skin as the
membrane.
Results: Transethosome F2 containing green tea extract equivalent to 3% EGCG, 4% Lipoid P30, 0.75% Span 80, and 30% ethanol had the best
characteristic including spherical shape, smallest particle size (35.35 nm), 0.319 PDI, zeta potential of −29.97±3.05 mV, and highest EE of
45.26%±8.15%. TEC permitted greater flux than non-TEC (first phase: 60.56±4.52 vs. 25.69±0.83 μg•cm−2•h−1 and second phase: 23.13±1.38 vs.
7.36±1.59 μg•cm−2•h−1).
Conclusion: Transethosome can increase the skin penetration of green tea leaf extract.

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References

Anand J, Upadhyaya B, Rawat P, Rai N. Biochemical characterization

and pharmacognostic evaluation of purified catechins in green tea

(Camellia sinensis) cultivars of India. Biotechnology 2015;5:285-94.

Lister E, Wilson P. Measurement of Total Phenolics and Abts Assay for

Antioxidant Activity Crop Lincoln. New Zealand: Research Institute;

Horzic D, Komes D, Belscak A, Ganic KK, Ivekovic D, Karlovic D.

The composition of polyphenols and methylxanthine in teas and herbal

infusions. Food Chem 2009;115:441-8.

Fujimura Y, Tachibana H, Maeda-Yamamoto M, Miyase T, Sano M,

Yamada K. Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-

O-methyl)-gallate, suppresses Fc-epsilon RI expression in human

basophilic KU812 cells. J Agric Food Chem 2002;50:5729-34.

Patel AR, Velikov KP. Non-covalent interactions of green tea

polyphenols: Source of novel colloidal structures. Nova Biomed

;2013:151-66.

Srivastava S, Maurya A, Gupta P. A review article on transdermal drug

delivery system. World J Pharm Pharm Sci 2016;5:1702-25.

Song CK, Balakrishnan P, Shim CK, Chung SJ, Chong S, Kim DD, et al.

A novel vesicular carrier, transethosome, for enhanced skin delivery of

voriconazole: Characterization and in vitro/in vivo evaluation. Colloids

Surf B Biointerfaces 2012;92:299-304.

Ascenso A, Raposo S, Batista C, Cardoso P, Mendes T, Praça FG,

et al. Development, characterization, and skin delivery studies of

related ultradeformable vesicles: Transfersomes, ethosomes, and

transethosomes. Int J Nanomedicine 2015;10:5837-51.

Allen LV, Popovich NG, Ansel HC. Ansel’s Pharmaceutical Dosage

Forms and Drug Delivery Systems. 9th ed. Philadelphia, PA: Lippincott

Williams and Wilkins; 2011.

Fangueiro JF, Parra A, Silva AM, Egea MA, Souto EB, Garcia ML, et al.

Validation of a high performance liquid chromatography method for the

stabilization of epigallocatechin gallate. Int J Pharm 2014;475:181-90.

Mishra K, Ojha H, Chaudhury NK. Estimation of antiradical properties

of antioxidants using DPPH assay: A critical review and results. Food

Chem 2012;130:1036-43.

Izzreen MN, Fadzelly AB. Phytochemicals and antioxidant properties

of different parts of Camellia sinensis leaves from Sabah tea plantation

in Sabah, Malaysia. Int Food Res J 2013;20:307-12

Zirak MB, Pezeshki A. Effect of surfactant concentration on the particle

size, stability and potential zeta of beta carotene nano lipid carrier. Int J

Curr Microbiol App Sci 2015;4:924-32.

Geetha S, Irulandi K, Mehalingam P. Evaluation of antioxidant and free

radical scavenging activities of different solvent extracts of leaves of

Piper umbellatum. Asian J Pharm Clin Res 2017;10:274-6.

Farhan H. Studying the cytotoxic and antioxidant activities of crude

extracts from Anacyclus ngellifolius boss grown in North Lebanon.

Asian J Pharm Clin Res 2018;11:376-80.

Srinivasan P, Jayagopal M, Ramachandran J. Evaluation of in-vitro

antioxidant and fibrinolytic activity of flavonoid-rich fraction from the

whole plant of Wedelia chinensis. Asian J Pharm Clin Res 2016;9:234‑9.

Touitou E, Dyan N, Bergelson L, Godin B, Eliaz M. Ethosomes–novel

vesicular carriers for enhanced delivery: Characterization and skin

penetration properties. J Control Release 2000;65:403-418.

Published

20-12-2018

How to Cite

Anwar, E., Ramadon, D., & Ardi, G. D. (2018). NOVEL TRANSETHOSOME CONTAINING GREEN TEA (CAMELLIA SINENSIS L. KUNTZE) LEAF EXTRACT FOR ENHANCED SKIN DELIVERY OF EPIGALLOCATECHIN GALLATE: FORMULATION AND IN VITRO PENETRATION TEST. International Journal of Applied Pharmaceutics, 10(1), 299–302. https://doi.org/10.22159/ijap.2018.v10s1.66

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