STUDY OF TYROSINASE INHIBITORY ACTIVITY AND PHYTOCHEMICAL SCREENING OF CASSIA FISTULA L. LEAVES
Keywords:Cassia fistula, L-DOPA, Phytochemical screening, Tyrosinase inhibitor
Objective: This study was carried out to evaluate the phytochemical constituents and tyrosinase inhibitory activity of Cassia fistula leaves.
Methods: A tyrosinase inhibitory activity assay was performed by measuring the decrease in the intensity of color suggestive of the inhibition of
dopachrome formation resulting from the L-DOPA-tyrosinase reaction.
Results: The test results showed that the tyrosinase inhibitory activity of the water fraction of C. fistula leaf extract had the highest IC50 value
(152.031 Î¼g/mL) among other fractions (n-hexane, ethyl acetate, and n-butanol). An enzyme kinetic assay showed that the water fraction of C. fistula
leaf extract inhibited tyrosinase with mixed-type inhibition. Phytochemical screening showed that the water fraction of C. fistula leaf extract contained
alkaloids, flavonoids, glycosides, phenols, and tannins.
Conclusion: The current study indicated that C. fistula leaves possess significant tyrosinase inhibitory activity.
Chang TS. An updated review of tyrosinase inhibitors. Int J Mol Sci
Kim YJ, Uyama H. Tyrosinase inhibitors from natural and synthetic
sources: Structure, inhibition mechanism and perspective for the future. Cell Mol Life Sci 2005;62:1707-23.
Smit N, Vicanova J, Pavel S. The hunt for natural skin whitening agents.
Int J Mol Sci 2009;10:5326-49.
Jothy SL, Zakaria Z, Chen Y, Lau YL, Latha LY, Sasidharan S, et al.
Acute oral toxicity of methanolic seed extract of Cassia fistula in mice.
Data of Cassia fistula L. 2012. Available from: http://www.
proseanet.org/prohati2/browser.php? docsid=137. [Last accessed on
Kaji NN, Khorana ML, Sanghavi MM. Studies on Cassia fistula Linn.
Indian J Pharm 1968;30:8-11.
Mahesh VK, Sharma R, Singh RS. Anthraquinones and kaempferol
from Cassia fistula species. J Nat Prod 1984;47:733-51.
Kuo YH, Lee PH, Wein YS. Four new compounds from the seeds of
Cassia fistula. J Nat Prod 2002;65:1165-7.
Narayanan V, Seshadri TR. Proanthocyanidins of Cassia fistula. Indian
J Chem 1972;10:379-81.
Kumar A, Pande CS, Kaul RK. Chemical examination of Cassia fistula
flowers. Indian J Chem 1966;4:460.
Senthil Kumar M, Sripriya R, Vijaya Raghavan H, Sehgal PK. Wound
healing potential of Cassia fistula on infected albino rat model. J Surg
Bhakta T, Mukherjee PK, Saha K, Pal M, Saha BP, Mandal SC.
Evaluation of anti-inflammatory effects of Cassia fistula (Leguminosae)
leaf extract on rats. J Herbs Spices Med Plants 1999;6:67-72.
Luximon-Ramma A, Bahorun T, Soobrattee MA, Aruoma OI. Antioxidant
activities of phenolic, proanthocyanidin, and flavonoid components in
extracts of Cassia fistula. J Agric Food Chem 2002;50:5042-7.
Pradeep K, Mohan CV, Anand KG, Karthikeyan S. Effect of
pretreatment of Cassia fistula Linn. leaf extract against subacute CCl4
induced hepatotoxicity in rats. Indian J Exp Biol 2005;43:526-30.
Duraipandiyan V, Ignacimuthu S. Antibacterial and antifungal activity
of Cassia fistula L.: An ethnomedicinal plant. J Ethnopharmacol
Wagner H, Bladt S. Plant Drug Analysis: A Thin Layer Chromatography
Atlas. 2nd ed. Berlin, Germany: Springer; 1996.
Farnsworth NR. Biological and phytochemical screening of plants.
J Pharm Sci 1966;55:225-76.
Evans WC. Trease and Evans Pharmacognosy. 15th ed. London,
United Kingdom: WB Saunders; 2002.
Harborne JB. Phytochemicals Method: Plant Analysis Guidance in
Modern Way. Bandung: ITB Publisher; 1987.
Sumathy V, Lachumy SJ, Zakaria Z, Sasidharan S. In vitro
bioactivity and phytochemical screening of Musa acuminata flower.
ISO 14502-1: 2005. Determination of Substances Characteristic
of Green and Black Tea. Part 1: Content of Total Polyphenols in
Tea. Colorimetric Method using Folin-Ciocalteu Reagent. Geneva,
Switzerland: ISO; 2005.
Jackson FS, Barry TN, Lascano C, Palmer B. The extractable and
bound condensed tannin contents of leaves from tropical tree, shrub
and forage legumes. J Sci Food Agric 1996;71:103-10.
Santos-Buelga C, Williamson G. Methods in Polyphenol Analysis.
London, United Kingdom: Royal Society of Chemistry; 2003.
Lin MC, Tsai MJ, Wen KC. Supercritical fluid extraction of flavonoids
from scutellariae radix. J Chromatogr A 1999;830:387-95.
Escarpa A, Morales MD, Gonzales MC. Analytical performance of
commercially available and unavailable phenolic compounds using
real samples by high-performance liquid chromatography-diode-array
detection. Anal Chim Acta 2002;460:61-72.
Patel A, Zaveri M. Preliminary screening of some selected plants for
antityrosinase activity. Int J Inst Pharm Life Sci 2012;2:213-9.
Rao GV, Rao KS, Annamalai T, Radhakrishnan N, Mukhopadhyay T.
Chemical constituents and mushroom tyrosinase inhibition activity of
Chloroxylon swietenia leaves. Turk J Chem 2009;33:521-6.
Ramanathan K, Shanthi V, Kanika V. Exploration of plant bioactive
from Cassia fistula leaves for the treatment of ovarian cancer: An
integrative approach. Asian J Pharm Clin Res 2016;9:182-8.
Fidrianny I, Harnovi M, Insanu M. Evaluation of antioxidant activities
from various extracts of sweet orange peels using DDPH, frap assays
and correlation with phenolic, flavonoid, carotenoid content. Asian J
Pharm Clin Res 2014;7:186-90.
Rahman MH, Alam MB, Hossain MS, Jha MK, Islam A. Antioxidant,
analgesic and toxic potentiality of methanolic extract of Stephania
japonica (Thunb.) Miers. leaf. Asian J Pharm Clin Res 2011;4:38-41.
Batubara I, Darusman LK, Mitsunaga T, Rahminiwati M, Djauhari E.
Potency of Indonesian medicinal plants as tyrosinase inhibitor and
antioxidant agent. J Biol Sci 2010;10:138-44.