STUDY OF TYROSINASE INHIBITORY ACTIVITY AND PHYTOCHEMICAL SCREENING OF CASSIA FISTULA L. LEAVES
DOI:
https://doi.org/10.22159/ijap.2018.v10s1.85Keywords:
Cassia fistula, L-DOPA, Phytochemical screening, Tyrosinase inhibitorAbstract
Objective: This study was carried out to evaluate the phytochemical constituents and tyrosinase inhibitory activity of Cassia fistula leaves.
Methods: A tyrosinase inhibitory activity assay was performed by measuring the decrease in the intensity of color suggestive of the inhibition of
dopachrome formation resulting from the L-DOPA-tyrosinase reaction.
Results: The test results showed that the tyrosinase inhibitory activity of the water fraction of C. fistula leaf extract had the highest IC50 value
(152.031 μg/mL) among other fractions (n-hexane, ethyl acetate, and n-butanol). An enzyme kinetic assay showed that the water fraction of C. fistula
leaf extract inhibited tyrosinase with mixed-type inhibition. Phytochemical screening showed that the water fraction of C. fistula leaf extract contained
alkaloids, flavonoids, glycosides, phenols, and tannins.
Conclusion: The current study indicated that C. fistula leaves possess significant tyrosinase inhibitory activity.
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