EFFICACY OF JAVANESE TURMERIC ETHANOL EXTRACT IN ERADICATING STREPTOCOCCUS SANGUINIS AND PORPHYROMONAS GINGIVALIS BIOFILM
DOI:
https://doi.org/10.22159/ijap.2019.v11s1.155Keywords:
Identified Javanese turmeric ethanol extract, Streptococcus sanguinis, Porphyromonas gingivalis, Biofilm eradicationAbstract
Objective: Javanese turmeric is an Indonesian native medicinal plant with antibacterial activities. This study aimed to analyze the efficacy of identified
Javanese turmeric ethanol extract (IJTEE) in eradicating Streptococcus sanguinis and Porphyromonas gingivalis biofilms.
Methods: Biofilm assay: Single and combination biofilms formed at different phases were exposed to IJTEE in 0.5–25% concentrations for 1 h. The
percentage of eradication was tested using the microtetrazolium assay.
Results: The efficacy of IJTEE in eradicating the biofilm was equal to that of chlorhexidine against the early phase of biofilm formation. IJTEE is more
effective against S. sanguinis biofilm formation than against P. gingivalis biofilm formation.
Conclusion: IJTEE can eradicate S. sanguinis and P. gingivalis biofilms.
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References
1. Solmaz G, Korachi M. Inhibition and disruption properties of
chlorhexidine gluconate on single and multispecies oral biofilms.
Jundishapur J Microbiol 2013;6:61-6.
2. Dewi F, Angky S, Azalia A. Dental Pharmacology. Jakarta: Badan
Penerbit FKUI; 2012.
3. Indonesian Agency of Food and Drugs Administration. National
Movement of Drinking Curcuma xanthorrhiza. 2005;6:1-12.
4. Hwang JK, Shim JS, Pyun YR. Antibacterial activity of xanthorrhizol
from Curcuma xanthorrhiza against oral pathogens. Fitoterapia
2000;71:321-3.
Int J App Pharm, Vol 11, Special Issue 1, 2019
5. Mono R. Standard operational procedure of turmeric cultivation as
potential raw material for herbal medicine. Perspektif 2010;9:78-93.
6. Cut N. Effects of Turmeric Ethanol Extract on Viability of Streptoccocus
sanguinis Based on MTT Test (in vitro). [Undergraduate Thesis].
Jakarta: Universitas Indonesia; 2011.
7. Mangunwardoyo W, Deasywaty, Usia T. Antimicrobial and
identification of active compound Curcuma xanthorrhiza Roxb. Int J
Basic Appl Sci IJENS 2012;12:69-78.
8. Marsh PD, Martin MV. Oral Microbiology. 5th ed. London: Churchill
Livingstone Elsevier; 2009. p. 32-7.
9. Newman MG, Takei H, Klokkevold PR, Carranza FA. Carranza’s
Clinical Periodontology. 10th ed. St. Louis, Missouri: Elsevier; 2006.
10. Samaranayake L. Essential Microbiology for Dentistry. 4th ed. London:
Churchill Livingstone Elsevier; 2012.
11. Tahmourespour A, Kermanshahi RK, Salehi R, Pero NG. Biofilm
formation potential of oral streptococci in related to some carbohydrate
substrates. Afr J Microbiol Res 2010;4:1051-6.
12. Marsh PD, Martin MV. Oral Microbiology. 5th ed. London: Churchill
Livingstone Elsevier; 2009. p. 78-85.
13. Kouidhi B, Al Qurashi YM, Chaieb K. Drug resistance of bacterial
dental biofilm and the potential use of natural compounds as alternative
for prevention and treatment. Microb Pathog 2015;80:39-49.
14. Hertiani T, Pratiwi SU, Irianto ID, Adityaningrum D, Pranoto B. Effect
of indonesian medicinal plants essential oils on Streptococcus mutans
biofilm. Indones J Pharm 2011;22:174-81.
15. Quave CL, Plano LR, Pantuso T, Bennett BC. Effects of extracts
from Italian medicinal plants on planktonic growth, biofilm formation
and adherence of Methicillin-resistant Staphylococcus aureus.
J Ethnopharmacol 2008;118:418-28.
16. Kolenbrander PE, Andersen RN, Blehert DS, Egland PG, Foster JS,
Palmer RJ. Communication among oral bacteria. Microbiol Mol Biol
Rev 2002;66:486-505.
17. Balagopal S, Arjunkumar R. Chlorhexidine: The gold standard
antiplaque agent. J Pharm Sci Res 2013;5:270-4.
18. Huang KT, Chen YH, Walker AM. Inaccuracies in MTS assays:
Major distorting effects of medium, serum albumin, and fatty acids.
Biotechniques 2004;37:406, 408, 410-2.
19. Stingu CS, Eschrich K, Rodloff AC, Schaumann R, Jentsch H.
Periodontitis is associated with a loss of colonization by Streptococcus
sanguinis. J Med Microbiol 2008;57:495-9.
20. Zhang JC, Zhou C, Wu B, Zhang YH. Investigation on interaction
between Streptococcus sanguis and Porphyromonas gingivalis in
specific pathogen-free rats. Chin J Dent Res 2000;3:5-9.
21. Ma S, Li H, Yan C, Wang D, Li H, Xia X, et al. Antagonistic effect of
protein extracts from Streptococcus sanguinis on pathogenic bacteria
and fungi of the oral cavity. Exp Ther Med 2014;7:1486-94.
chlorhexidine gluconate on single and multispecies oral biofilms.
Jundishapur J Microbiol 2013;6:61-6.
2. Dewi F, Angky S, Azalia A. Dental Pharmacology. Jakarta: Badan
Penerbit FKUI; 2012.
3. Indonesian Agency of Food and Drugs Administration. National
Movement of Drinking Curcuma xanthorrhiza. 2005;6:1-12.
4. Hwang JK, Shim JS, Pyun YR. Antibacterial activity of xanthorrhizol
from Curcuma xanthorrhiza against oral pathogens. Fitoterapia
2000;71:321-3.
Int J App Pharm, Vol 11, Special Issue 1, 2019
5. Mono R. Standard operational procedure of turmeric cultivation as
potential raw material for herbal medicine. Perspektif 2010;9:78-93.
6. Cut N. Effects of Turmeric Ethanol Extract on Viability of Streptoccocus
sanguinis Based on MTT Test (in vitro). [Undergraduate Thesis].
Jakarta: Universitas Indonesia; 2011.
7. Mangunwardoyo W, Deasywaty, Usia T. Antimicrobial and
identification of active compound Curcuma xanthorrhiza Roxb. Int J
Basic Appl Sci IJENS 2012;12:69-78.
8. Marsh PD, Martin MV. Oral Microbiology. 5th ed. London: Churchill
Livingstone Elsevier; 2009. p. 32-7.
9. Newman MG, Takei H, Klokkevold PR, Carranza FA. Carranza’s
Clinical Periodontology. 10th ed. St. Louis, Missouri: Elsevier; 2006.
10. Samaranayake L. Essential Microbiology for Dentistry. 4th ed. London:
Churchill Livingstone Elsevier; 2012.
11. Tahmourespour A, Kermanshahi RK, Salehi R, Pero NG. Biofilm
formation potential of oral streptococci in related to some carbohydrate
substrates. Afr J Microbiol Res 2010;4:1051-6.
12. Marsh PD, Martin MV. Oral Microbiology. 5th ed. London: Churchill
Livingstone Elsevier; 2009. p. 78-85.
13. Kouidhi B, Al Qurashi YM, Chaieb K. Drug resistance of bacterial
dental biofilm and the potential use of natural compounds as alternative
for prevention and treatment. Microb Pathog 2015;80:39-49.
14. Hertiani T, Pratiwi SU, Irianto ID, Adityaningrum D, Pranoto B. Effect
of indonesian medicinal plants essential oils on Streptococcus mutans
biofilm. Indones J Pharm 2011;22:174-81.
15. Quave CL, Plano LR, Pantuso T, Bennett BC. Effects of extracts
from Italian medicinal plants on planktonic growth, biofilm formation
and adherence of Methicillin-resistant Staphylococcus aureus.
J Ethnopharmacol 2008;118:418-28.
16. Kolenbrander PE, Andersen RN, Blehert DS, Egland PG, Foster JS,
Palmer RJ. Communication among oral bacteria. Microbiol Mol Biol
Rev 2002;66:486-505.
17. Balagopal S, Arjunkumar R. Chlorhexidine: The gold standard
antiplaque agent. J Pharm Sci Res 2013;5:270-4.
18. Huang KT, Chen YH, Walker AM. Inaccuracies in MTS assays:
Major distorting effects of medium, serum albumin, and fatty acids.
Biotechniques 2004;37:406, 408, 410-2.
19. Stingu CS, Eschrich K, Rodloff AC, Schaumann R, Jentsch H.
Periodontitis is associated with a loss of colonization by Streptococcus
sanguinis. J Med Microbiol 2008;57:495-9.
20. Zhang JC, Zhou C, Wu B, Zhang YH. Investigation on interaction
between Streptococcus sanguis and Porphyromonas gingivalis in
specific pathogen-free rats. Chin J Dent Res 2000;3:5-9.
21. Ma S, Li H, Yan C, Wang D, Li H, Xia X, et al. Antagonistic effect of
protein extracts from Streptococcus sanguinis on pathogenic bacteria
and fungi of the oral cavity. Exp Ther Med 2014;7:1486-94.
Published
05-04-2019
How to Cite
YANUAR, R., SUNIARTI, D. F., & DJOHAN, W. (2019). EFFICACY OF JAVANESE TURMERIC ETHANOL EXTRACT IN ERADICATING STREPTOCOCCUS SANGUINIS AND PORPHYROMONAS GINGIVALIS BIOFILM. International Journal of Applied Pharmaceutics, 11(1), 18–22. https://doi.org/10.22159/ijap.2019.v11s1.155
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