EFFECTIVENESS OF IDENTIFIED JAVANESE TURMERIC ETHANOL EXTRACT FOR THE INHIBITION OF BIOFILM FORMATION BY STREPTOCOCCUS MUTANS AND PORPHYROMONAS GINGIVALIS
DOI:
https://doi.org/10.22159/ijap.2019.v11s1.156Keywords:
Biofilm, identified Javanese turmeric ethanol extract, Minimum bactericidal concentration, Minimum inhibitory concentration, Porphyromonas gingivalis, Streptococcus mutansAbstract
Objective: To study the effectiveness of identified Javanese turmeric ethanol extract (IJTEE) against single and combined biofilm formation by
Streptococcus mutans and Porphyromonas gingivalis.
Methods: S. mutans ATCC 25175 and P. gingivalis ATCC 33277 were tested for the minimum inhibitory concentration (MIC) and minimum bactericidal
concentration (MBC) of IJTEE using microdilution technique. The inhibition of biofilm formation by IJTEE was analyzed using crystal violet assay.
Results: MIC and MBC of IJTEE for S. mutans were 5% and 15%, respectively. MIC of IJTEE for the biofilm of S. mutans was 1% and for that of
P. gingivalis was 15%; the MIC of IJTEE for the combined biofilm was 0.5%.
Conclusion: IJTEE was effective in inhibiting single and combined biofilm formation by S. mutans and P. gingivalis.
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15. Tu Y, Ling X, Chen Y, Wang Y, Zhou N, Chen H. The supernatans of
S. mutans and S. sanguinis significantly inhibit the growth and biofilm
formation P. gingivalis. Med Sci Monit 2017;23:5439-45.
Ariati SR, et al. Bioresources for green economics development.
Jakarta: LIPI Press; 2013. p. 3-8.
2. Nugraha AS, Keller PA. Revealing indigenous Indonesian traditional
medicine: Anti-infective agents. Nat Prod Commun 2011;6:1953-66.
3. Dewoto HR. The development of Indonesian herbal medicine as
phytopharmacy. Maj Kedokt Indones 2007;57:205-11.
4. Hayani E. Analysis of Chemical Contents in Javanese Turmeric. Jakarta:
Pusat Penelitian dan Pengembangan Pertanian; 2006. p. 309-12.
5. Hwang JK, Shim JS, Pyun YR. Antibacterial activity of xanthorrhizol
from Curcuma xanthorrhiza against oral pathogens. Fitoterapia
2000;71:321-3.
6. Metwalli KH, Khan SA, Krom BP, Jabra-Rizk MA. Streptococcus
mutans, Candida albicans, and the human mouth: A sticky situation.
PLoS Pathog 2013;9:e1003616.
7. Marsh PD, Martin MV. Oral Microbiology. 5th ed. Philadelphia, PA:
Churchill Livingstone; 2009. p. 8-103.
8. Solmaz G, Korachi M. Inhibition and disruption properties of
chlorhexidine gluconate on single and multispecies oral biofilms.
Jundishapur J Microbiol 2013;6:61-6.
9. Lee LY, Shim JS, Rukayadi Y, Hwang JK. Antibacterial activity of
xanthorrhizol isolated from Curcuma xanthorrhiza Roxb. Against
foodborne pathogens. J Food Prot 2008;71:1926-30.
10. Hertiani T, Pratiwi S. Effect of Indonesian medicinal plants essential oils
on Streptococcus mutans biofilm. Maj Farm Indones 2011;22:174-81.
11. A Algarni A, H Yassen G, L Gregory R. Inhibitory effect of gels loaded
with a low concentration of antibiotics against biofilm formation by
Enterococcus faecalis and Porphyromonas gingivalis. J Oral Sci
2015;57:213-8.
12. Suwandi T, Suniarti DF, Prayitno SW. Effect of ethanol extract of
Hibiscus sabdariffa L. calyx on Streptococcus sanguinis viability in vitro
biofilm based on crystal violet. J Med Plants Res 2013;7:2476-82.
13. Busri A. Antibacterial Effect of Javanese turmeric Ethanol Extract on
the Dual Species of Streptococcus mutans and Streptococcus sanguinis
(in vitro). Undergraduate Thesis. Jakarta: Universitas Indonesia; 2013.
14. Mangunwardoyo W, Usia TD. Antimicrobial and identification of
active compound Curcuma xanthorrhiza Roxb. Int J Basic Appl Sci
2012;12:69-78.
15. Tu Y, Ling X, Chen Y, Wang Y, Zhou N, Chen H. The supernatans of
S. mutans and S. sanguinis significantly inhibit the growth and biofilm
formation P. gingivalis. Med Sci Monit 2017;23:5439-45.
Published
05-04-2019
How to Cite
MARPAUNG, L. N. F., SUNIARTI, D. F., & SARWONO, A. T. (2019). EFFECTIVENESS OF IDENTIFIED JAVANESE TURMERIC ETHANOL EXTRACT FOR THE INHIBITION OF BIOFILM FORMATION BY STREPTOCOCCUS MUTANS AND PORPHYROMONAS GINGIVALIS. International Journal of Applied Pharmaceutics, 11(1), 23–26. https://doi.org/10.22159/ijap.2019.v11s1.156
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